Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   O1449_RS01530 Genome accession   NZ_CP114264
Coordinates   317766..319481 (-) Length   571 a.a.
NCBI ID   WP_269228985.1    Uniprot ID   A0A9E9L583
Organism   Acinetobacter sp. TR3     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 312766..324481
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  O1449_RS01500 (O1449_01500) - 312852..313178 (+) 327 WP_004659291.1 pyrimidine/purine nucleoside phosphorylase -
  O1449_RS01505 (O1449_01505) rlmB 313360..314109 (+) 750 WP_269238962.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  O1449_RS01510 (O1449_01510) - 314140..315051 (+) 912 WP_269238963.1 DMT family transporter -
  O1449_RS01515 (O1449_01515) coaE 315048..315653 (-) 606 WP_269238964.1 dephospho-CoA kinase -
  O1449_RS01520 (O1449_01520) pilD 315655..316515 (-) 861 WP_269228987.1 A24 family peptidase Machinery gene
  O1449_RS01525 (O1449_01525) pilC 316515..317741 (-) 1227 WP_269228986.1 type II secretion system F family protein Machinery gene
  O1449_RS01530 (O1449_01530) pilB 317766..319481 (-) 1716 WP_269228985.1 type IV-A pilus assembly ATPase PilB Machinery gene
  O1449_RS01535 (O1449_01535) tpiA 319826..320617 (+) 792 WP_269238965.1 triose-phosphate isomerase -
  O1449_RS01540 (O1449_01540) secG 320630..320959 (+) 330 WP_005162938.1 preprotein translocase subunit SecG -
  O1449_RS01560 (O1449_01560) rimP 321585..322109 (+) 525 WP_269228983.1 ribosome maturation factor RimP -
  O1449_RS01565 (O1449_01565) nusA 322147..323631 (+) 1485 WP_269238967.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 63242.53 Da        Isoelectric Point: 5.4233

>NTDB_id=765288 O1449_RS01530 WP_269228985.1 317766..319481(-) (pilB) [Acinetobacter sp. TR3]
MSGLHTSPKFSGFIRRLVEDGHMTAANMQNAIDAAKKAKQDIVAHLIEQHRLSPLTIAESISVEFGEPLFDLAVYDVGLL
PRELTDNKLIQKHRVVPLIQRGQILYVATSNPTNIDALDAIRFNTKLNIEPIIVEHTKLEKLIEQHFGDSGSFDFGDSED
FDLDIDVNQDSPQENDDNSSQGDESPIVKYINKLLIDAIRMGASDLHFEPYEKTYRVRYRVDGVLRQIATPPLQMANRLA
SRLKVMSQMDISEKRMPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIEALGYEDDQKALFIEALEK
PQGMLLITGPTGSGKTVSLYTGLNILNTEDTNISTAEDPVEINLEGINQVNVNPKTGLTFSAALKAFLRQDPDVIMVGEI
RDLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKIPLNVPKQSLIELG
FTEQDLANPDLQIFQPIGCPECREGYKGRVGIYEVMKVTPAISKIIMEDGNALQIAAASEQAGFNNLRRSGLKKVMQGIT
SLQEINRVTSE

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=765288 O1449_RS01530 WP_269228985.1 317766..319481(-) (pilB) [Acinetobacter sp. TR3]
ATGTCAGGATTACATACGTCTCCAAAGTTTTCGGGGTTCATTCGACGTTTAGTTGAAGATGGTCATATGACTGCTGCAAA
TATGCAGAATGCAATTGATGCAGCCAAGAAAGCCAAACAAGATATTGTTGCTCATTTGATTGAACAACATCGTCTTTCTC
CACTTACCATTGCAGAAAGCATCTCCGTAGAATTTGGCGAACCACTTTTTGATTTGGCGGTTTACGATGTTGGATTATTA
CCTCGAGAATTAACCGACAATAAACTCATACAAAAACATCGTGTTGTTCCGTTAATACAACGTGGACAAATTTTGTATGT
TGCTACGAGCAACCCAACTAATATTGATGCATTAGATGCAATTAGGTTCAATACGAAACTTAATATTGAACCAATTATTG
TTGAGCATACGAAACTTGAAAAGTTAATCGAACAACATTTCGGTGATTCAGGTAGTTTCGATTTTGGTGACAGTGAAGAC
TTCGATTTAGATATCGATGTTAATCAAGATTCTCCTCAAGAAAATGACGATAATTCATCTCAAGGTGATGAATCGCCTAT
TGTTAAATATATTAATAAGCTTTTAATTGATGCAATTCGCATGGGCGCATCAGATTTACATTTTGAACCTTACGAAAAAA
CGTATCGTGTGCGTTATCGTGTAGATGGCGTTCTACGCCAAATTGCTACCCCACCATTACAAATGGCAAATCGCTTGGCT
TCTCGTTTAAAAGTTATGTCCCAAATGGATATTTCAGAAAAACGAATGCCTCAAGATGGTCGTATTAAACTCAAGTTATC
AAAAACCAAAGCAATTGATTTCCGTGTTAACTCTTTACCTACTTTATTTGGAGAAAAATTAGTACTACGTATTTTGGATC
CATCAAGTGCTATGCTCGGGATTGAAGCATTAGGTTATGAAGATGATCAGAAAGCATTATTCATTGAAGCACTTGAAAAG
CCTCAAGGAATGCTATTGATTACAGGTCCAACAGGTTCTGGTAAAACCGTATCGCTATATACTGGTCTAAATATTCTAAA
CACTGAAGATACCAATATTTCAACAGCAGAAGACCCTGTTGAAATTAACTTGGAAGGCATTAATCAGGTCAACGTGAATC
CAAAGACGGGATTAACTTTCTCTGCTGCATTAAAGGCTTTCTTACGCCAAGACCCGGATGTAATTATGGTCGGTGAGATT
CGTGATTTGGAAACAGCCGAAATTGCAATTAAAGCAGCGCAAACAGGTCATATGGTGATGTCAACACTGCATACCAACAG
TGCGCCAGAAACTTTAACTCGTTTACGCAACATGGGTGTACCTTCGTTTAATATCGCAACATCAGTTAATCTTGTTATCG
CACAACGTTTAGCACGACGTTTATGCTCACAATGTAAAATACCTCTTAATGTACCTAAGCAGAGTCTCATTGAACTAGGA
TTTACTGAACAAGATTTAGCCAATCCAGATTTACAAATATTTCAGCCTATTGGATGTCCTGAATGCCGTGAAGGGTATAA
AGGGCGAGTAGGTATTTATGAAGTCATGAAGGTCACCCCCGCAATCTCTAAAATTATCATGGAAGATGGAAATGCACTGC
AAATTGCAGCAGCATCAGAACAAGCAGGGTTTAATAATCTCCGGCGTTCTGGTTTGAAAAAAGTAATGCAAGGAATTACC
TCATTGCAGGAAATTAACCGAGTAACAAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(193-460)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

82.487

100

0.825
  pilB Acinetobacter baylyi ADP1

77.058

100

0.771
  pilB Legionella pneumophila strain ERS1305867

53.775

95.096

0.511
  pilB Vibrio parahaemolyticus RIMD 2210633

49.286

98.074

0.483
  pilF Neisseria gonorrhoeae MS11

48.33

99.65

0.482
  pilB Vibrio campbellii strain DS40M4

48.837

97.898

0.478
  pilB Vibrio cholerae strain A1552

51.257

90.543

0.464
  pilB/pilB1 Synechocystis sp. PCC 6803

34.804

100

0.373
  pilF Thermus thermophilus HB27

37.5

98.074

0.368
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.548

92.995

0.368