Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   O1Q77_RS01940 Genome accession   NZ_CP114182
Coordinates   399905..401611 (-) Length   568 a.a.
NCBI ID   WP_005336134.1    Uniprot ID   -
Organism   Aeromonas veronii strain AS1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 394905..406611
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  O1Q77_RS01910 (O1Q77_01910) mutT 395416..395820 (+) 405 WP_019444787.1 8-oxo-dGTP diphosphatase MutT -
  O1Q77_RS01915 (O1Q77_01915) yacG 395898..396092 (-) 195 WP_069527075.1 DNA gyrase inhibitor YacG -
  O1Q77_RS01920 (O1Q77_01920) zapD 396102..396824 (-) 723 WP_005336141.1 cell division protein ZapD -
  O1Q77_RS01925 (O1Q77_01925) coaE 396868..397476 (-) 609 WP_304520465.1 dephospho-CoA kinase -
  O1Q77_RS01930 (O1Q77_01930) pilD 397490..398362 (-) 873 WP_304520466.1 A24 family peptidase Machinery gene
  O1Q77_RS01935 (O1Q77_01935) pilC 398424..399665 (-) 1242 WP_005355091.1 type II secretion system F family protein Machinery gene
  O1Q77_RS01940 (O1Q77_01940) pilB 399905..401611 (-) 1707 WP_005336134.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  O1Q77_RS01945 (O1Q77_01945) - 401616..402017 (-) 402 WP_120416063.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  O1Q77_RS01950 (O1Q77_01950) nadC 402333..403196 (-) 864 WP_279464204.1 carboxylating nicotinate-nucleotide diphosphorylase -
  O1Q77_RS01955 (O1Q77_01955) - 403200..403670 (-) 471 WP_040068932.1 TIGR02281 family clan AA aspartic protease -
  O1Q77_RS01960 (O1Q77_01960) ampD 403801..404376 (+) 576 WP_236280279.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  O1Q77_RS01965 (O1Q77_01965) pdhR 404655..405419 (+) 765 WP_005336127.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62533.87 Da        Isoelectric Point: 5.3192

>NTDB_id=764833 O1Q77_RS01940 WP_005336134.1 399905..401611(-) (pilB) [Aeromonas veronii strain AS1]
MTSSPNSGLALSLAASSLISEEDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTAAIGKLLESENDALGMEDIDESEI
SDLEVSDENSRLDESVNTADDDAPIVKYINKILMDAIKRGASDLHFEPYEFKYRVRFRIDGILHEIATPPVNLSNRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
PQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EVNRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=764833 O1Q77_RS01940 WP_005336134.1 399905..401611(-) (pilB) [Aeromonas veronii strain AS1]
ATGACCTCTAGCCCTAATAGCGGCCTGGCGTTAAGCCTGGCCGCCTCTTCACTGATCAGTGAAGAGGATTCCCAGCGCTA
TCTGAGCCAGGCCAAGGCCCAGCGCAAACCCTTTGTGACATTTCTGATAGAGAACGAGATCCTCGACAGCAAGGCGCTCG
CCGACTTTTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACTTGGCCGAGATCCCGCAGAAGTAC
CTCAATCAGAAGCTGATTGAGAAACACCATGTGCTGCCCATCTACACCCAGGGCCACACCCTCTATATCGCCATGTCGGA
TCCGACCAATGTCTCGGCGTTGGAGGACTTTGGCTTCAGCTTCGGGCTTCATACCGAGGCGCTGCTGGTGGAGGAGAACA
AGCTCACCGCCGCCATCGGCAAGCTGCTGGAGAGCGAAAACGACGCCCTCGGCATGGAGGATATCGACGAATCCGAGATT
TCGGATCTCGAGGTCTCCGACGAGAACAGCCGGCTGGACGAAAGCGTCAATACCGCCGATGACGATGCTCCCATCGTCAA
GTACATCAACAAGATCCTGATGGATGCCATCAAGCGCGGTGCCTCCGACCTGCATTTCGAGCCCTATGAGTTCAAGTATC
GGGTGCGCTTTCGCATCGATGGCATTCTGCATGAGATCGCCACACCGCCGGTTAACCTCTCCAACCGCTTCTCCGCTCGC
CTCAAGGTGATGGCGCGGCTCGATATCGCCGAGCGGCGGCTGCCGCAGGATGGCCGCATCAAGTTGAAGCTGTCGCGCAA
CAAGTCGATGGATATGCGGGTCAACACCCTGCCCACCATGTGGGGGGAGAAGATCGTTATCCGTCTGCTCGACTCCTCGG
CGGCACGCCTCAATATCGAGCAACTCGGCTTTGATGACCGGCAGAAGGCGCAGTACCTGCGCGCCCTCTCCAAACCGCAG
GGGATGATCCTGGTGACCGGCCCCACCGGCTCGGGCAAAACGGTCTCGCTCTATACCGGTCTCAATATTCTCAACACCAC
CGAGGTAAATATCTCCACCGCCGAAGATCCGGTGGAGATCAACTTGCCCGGCGTCAACCAGGTGCAGGTCAACCCCAAGG
CGGGGCTCACCTTTGCCAGCGCCCTGCGCTCCTTCTTGCGGCAAGACCCGGATGTGGTGATGGTGGGTGAAATTCGCGAT
CTCGAAACCGCCGAGATCGCCATCAAGGCGGCCCAGACCGGCCACCTGGTGCTCTCCACCCTGCATACCAACTCCGCCGC
CGAGACCCTGACCCGGATGATGAACATGGGGGTGCCCGCCTTCAACATCGCCTCCTCGGTGACCCTGATCATGGCCCAGC
GGTTGGCCCGTAAACTGTGCGACCACTGCAAGGCGCCGGAAGTGGTGCCGGAAGCCGAACTGCTGGAGCTGGGCTTTACG
CCGCAGCAGCTGGCAGCCGGGCTGCGACTGTTCAAACCGGTCGGTTGCAAGGAGTGTTCCGGCGGCTACAAGGGGCGGGT
CGGCATCTACGAAATCATGCTGATGTCAGAGAACATCGCCAAATTGATCATGCAGGGGGCCAACTCCCTGCAAATCGCCG
CTATCGCGCAGAAGGAGGGGATGCGTACCTTACGTACCTCGGGACTGGAGAAGGCCCGGCTCGGGGTCACCAGTCTAGCC
GAGGTCAACCGGGTCACCACCAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.986

100

0.56
  pilB Acinetobacter baylyi ADP1

55.789

100

0.56
  pilB Legionella pneumophila strain ERS1305867

56.642

95.423

0.54
  pilB Vibrio cholerae strain A1552

54.758

98.063

0.537
  pilB Vibrio campbellii strain DS40M4

51.22

100

0.518
  pilB Vibrio parahaemolyticus RIMD 2210633

51.596

99.296

0.512
  pilF Neisseria gonorrhoeae MS11

46.809

99.296

0.465
  pilB/pilB1 Synechocystis sp. PCC 6803

39.06

97.359

0.38
  pilF Thermus thermophilus HB27

40.53

92.958

0.377