Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   OTS06_RS20345 Genome accession   NZ_CP113533
Coordinates   4336473..4338179 (+) Length   568 a.a.
NCBI ID   WP_324019718.1    Uniprot ID   -
Organism   Aeromonas hydrophila strain SHSK47     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4331473..4343179
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OTS06_RS20320 pdhR 4332589..4333353 (-) 765 WP_011707564.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  OTS06_RS20325 ampD 4333629..4334201 (-) 573 WP_324019715.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  OTS06_RS20330 - 4334348..4334818 (+) 471 WP_282464816.1 retropepsin-like aspartic protease -
  OTS06_RS20335 nadC 4334824..4335681 (+) 858 WP_282464815.1 carboxylating nicotinate-nucleotide diphosphorylase -
  OTS06_RS20340 - 4336004..4336468 (+) 465 WP_139748843.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  OTS06_RS20345 pilB 4336473..4338179 (+) 1707 WP_324019718.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  OTS06_RS20350 pilC 4338295..4339536 (+) 1242 WP_324019719.1 type II secretion system F family protein Machinery gene
  OTS06_RS20355 pilD 4339559..4340431 (+) 873 WP_324019720.1 A24 family peptidase Machinery gene
  OTS06_RS20360 coaE 4340451..4341065 (+) 615 WP_324019722.1 dephospho-CoA kinase -
  OTS06_RS20365 zapD 4341103..4341825 (+) 723 WP_024945431.1 cell division protein ZapD -
  OTS06_RS20370 yacG 4341835..4342029 (+) 195 WP_005305661.1 DNA gyrase inhibitor YacG -
  OTS06_RS20375 mutT 4342090..4342503 (-) 414 WP_016352173.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62564.95 Da        Isoelectric Point: 5.4342

>NTDB_id=762825 OTS06_RS20345 WP_324019718.1 4336473..4338179(+) (pilB) [Aeromonas hydrophila strain SHSK47]
MTSSPNSGLALSLAASSLLSESDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTTAIAKLLENDQDALGMEDIDESEI
SELEVSDEGSRLDESVNTADDDAPIVKYINKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEIATPPVNLANRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
QQQLTAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EINRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=762825 OTS06_RS20345 WP_324019718.1 4336473..4338179(+) (pilB) [Aeromonas hydrophila strain SHSK47]
ATGACCTCTAGCCCCAATAGCGGCCTGGCGTTAAGCTTGGCCGCCTCTTCCCTGCTCAGTGAGTCCGACTCACAACGCTA
TCTGAGCCAAGCCAAGGCTCAGCGTAAACCCTTTGTGACCTTTCTGATCGAAAATGAAATCCTCGACAGCAAAGCATTGG
CTGACTTCTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACCTGGCCGAGATCCCGCAGAAATAC
CTCAATCAGAAGCTGATTGAAAAACATCATGTCCTGCCCATCTACACCCAGGGGCACACCCTCTATATCGCCATGTCAGA
CCCGACCAATGTGTCAGCACTGGAGGATTTTGGTTTCAGCTTCGGGTTGCATACCGAGGCGCTGCTGGTCGAAGAGAACA
AGCTGACGACTGCCATCGCCAAGTTGCTGGAAAATGATCAGGATGCCCTCGGCATGGAGGACATCGACGAATCCGAGATC
TCCGAACTGGAGGTCTCGGATGAGGGGTCGCGGCTCGACGAGAGCGTCAATACGGCCGACGATGACGCGCCGATCGTCAA
ATACATCAACAAAATCATGATGGATGCCATCAAGCGCGGAGCCTCCGACCTTCACTTCGAGCCCTATGAGACCAAATATC
GCATCCGCTTTCGGATAGACGGGATCTTGCATGAAATAGCAACTCCGCCAGTCAATCTGGCCAACCGCTTCTCCGCCCGC
CTCAAGGTCATGGCACGGCTCGACATTGCCGAACGGCGACTGCCCCAAGATGGTCGTATCAAACTGAAGCTGTCGCGCAA
CAAGTCGATGGATATGCGGGTCAATACCCTACCCACCATGTGGGGCGAAAAAATCGTTATCCGTCTGCTCGACTCCTCCG
CCGCACGCCTCAACATTGAGCAGCTTGGTTTCGATGATCGGCAAAAAGCGCAATATCTGCGTGCTCTATCGAAGCCTCAG
GGCATGATCCTGGTAACCGGCCCTACCGGTTCGGGCAAGACGGTTTCACTCTATACCGGCCTCAATATTCTTAATACCAC
CGAGGTCAATATCTCCACCGCCGAAGATCCGGTGGAGATCAACCTGCCCGGCGTCAACCAGGTGCAGGTCAATCCCAAAG
CCGGGCTGACCTTCGCCAGCGCCCTGCGCTCTTTCTTGCGCCAGGACCCCGACGTAGTCATGGTGGGTGAAATCCGGGAT
CTGGAAACAGCCGAAATTGCCATCAAGGCCGCCCAGACCGGCCACCTGGTGCTATCCACCCTGCACACCAACTCAGCGGC
GGAAACGCTGACCCGGATGATGAACATGGGGGTGCCCGCCTTCAATATCGCCTCCTCGGTCACCCTGATCATGGCTCAAC
GGCTGGCGCGTAAACTGTGCGATCACTGCAAAGCTCCCGAAGTGGTGCCAGAGGCTGAGCTGCTTGAGCTGGGTTTTACC
CAGCAGCAGCTGACCGCTGGCTTGCGGCTGTTCAAACCGGTTGGCTGCAAAGAGTGTTCGGGTGGCTACAAGGGCCGGGT
CGGCATCTACGAGATCATGCTAATGTCTGAAAACATCGCAAAATTGATCATGCAGGGGGCCAACTCTCTGCAAATTGCCG
CCATCGCCCAGAAGGAGGGCATGCGCACCTTGCGCACCTCCGGCCTTGAGAAGGCCCGCCTGGGGGTGACCAGTCTGGCG
GAGATAAACCGGGTAACAACCAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-145)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.263

100

0.555
  pilB Acinetobacter baumannii D1279779

55.36

100

0.555
  pilB Legionella pneumophila strain ERS1305867

54.24

99.648

0.54
  pilB Vibrio cholerae strain A1552

54.676

97.887

0.535
  pilB Vibrio campbellii strain DS40M4

51.943

99.648

0.518
  pilB Vibrio parahaemolyticus RIMD 2210633

51.601

98.944

0.511
  pilF Neisseria gonorrhoeae MS11

46.809

99.296

0.465
  pilB/pilB1 Synechocystis sp. PCC 6803

38.98

96.655

0.377
  pilF Thermus thermophilus HB27

40.152

92.958

0.373