Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   OTS06_RS20355 Genome accession   NZ_CP113533
Coordinates   4339559..4340431 (+) Length   290 a.a.
NCBI ID   WP_324019720.1    Uniprot ID   -
Organism   Aeromonas hydrophila strain SHSK47     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4334559..4345431
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OTS06_RS20335 nadC 4334824..4335681 (+) 858 WP_282464815.1 carboxylating nicotinate-nucleotide diphosphorylase -
  OTS06_RS20340 - 4336004..4336468 (+) 465 WP_139748843.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  OTS06_RS20345 pilB 4336473..4338179 (+) 1707 WP_324019718.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  OTS06_RS20350 pilC 4338295..4339536 (+) 1242 WP_324019719.1 type II secretion system F family protein Machinery gene
  OTS06_RS20355 pilD 4339559..4340431 (+) 873 WP_324019720.1 A24 family peptidase Machinery gene
  OTS06_RS20360 coaE 4340451..4341065 (+) 615 WP_324019722.1 dephospho-CoA kinase -
  OTS06_RS20365 zapD 4341103..4341825 (+) 723 WP_024945431.1 cell division protein ZapD -
  OTS06_RS20370 yacG 4341835..4342029 (+) 195 WP_005305661.1 DNA gyrase inhibitor YacG -
  OTS06_RS20375 mutT 4342090..4342503 (-) 414 WP_016352173.1 8-oxo-dGTP diphosphatase MutT -
  OTS06_RS20380 - 4342512..4343696 (-) 1185 WP_346814951.1 tetratricopeptide repeat protein -

Sequence

Protein


Download         Length: 290 a.a.        Molecular weight: 32598.91 Da        Isoelectric Point: 6.7410

>NTDB_id=762827 OTS06_RS20355 WP_324019720.1 4339559..4340431(+) (pilD) [Aeromonas hydrophila strain SHSK47]
MLLITDVFHSLPWLYFSLVFLFSLMIGSFLNVVIHRLPIMLEREWQAEYLGYFNPETQPQQEERYNLMVPRSACPHCGHA
ITAMENIPLLSWLWLKGRCRECQAPISARYPLVELLTSLLSLIVAATFPPGWGLLAALLLTWVLVALTFIDLDKMLLPDQ
LTLPLLWGGLLFNLTGGFVPLADAVIGTMAGYLVLWSLYWAFKLLTGKEGMGYGDFKLLAALGAWLGWQALPIVLLLSSL
VGAFIGIGLILLRNHHQNKPIPFGPYLAIAGWIALLWGDTITRWYLTTFL

Nucleotide


Download         Length: 873 bp        

>NTDB_id=762827 OTS06_RS20355 WP_324019720.1 4339559..4340431(+) (pilD) [Aeromonas hydrophila strain SHSK47]
ATGTTGCTGATCACTGACGTTTTTCACAGCCTGCCCTGGCTCTATTTCTCGCTGGTATTCCTCTTTTCTCTGATGATCGG
CAGTTTTCTCAACGTGGTCATCCATCGCCTGCCCATCATGCTGGAGCGGGAGTGGCAGGCCGAATATCTCGGCTATTTCA
ATCCGGAAACACAGCCGCAGCAGGAAGAGCGCTACAACCTGATGGTGCCGCGTTCTGCCTGTCCCCATTGCGGCCACGCC
ATCACGGCCATGGAGAACATTCCCCTGCTCAGCTGGCTCTGGCTCAAAGGGCGCTGCCGTGAATGCCAGGCCCCCATCTC
GGCCCGTTATCCTCTGGTGGAACTGCTGACATCCCTGCTCTCGCTGATCGTCGCCGCAACATTCCCACCGGGCTGGGGCT
TGCTGGCCGCCCTGCTGCTCACCTGGGTACTGGTGGCACTCACTTTCATCGATCTGGACAAGATGCTTCTGCCGGATCAG
CTGACCCTGCCACTGCTGTGGGGCGGCCTGCTGTTCAACCTGACAGGGGGATTTGTACCACTGGCTGATGCGGTGATCGG
CACCATGGCCGGCTATCTGGTGCTCTGGAGCCTCTACTGGGCCTTCAAGCTGCTGACCGGCAAGGAAGGGATGGGCTATG
GTGACTTCAAGCTGCTGGCGGCGCTGGGTGCCTGGCTCGGCTGGCAGGCACTGCCCATCGTGTTGCTGCTCTCGTCGCTG
GTTGGCGCTTTCATCGGGATTGGTCTCATCTTGCTGCGCAACCATCATCAGAACAAACCCATCCCCTTCGGCCCCTATCT
GGCCATCGCCGGCTGGATCGCGCTGCTGTGGGGCGATACCATCACCCGCTGGTACCTCACGACGTTTCTCTGA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(139-247)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-128)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

60.49

98.621

0.597
  pilD Vibrio campbellii strain DS40M4

55.245

98.621

0.545
  pilD Acinetobacter baumannii D1279779

53.873

97.931

0.528
  pilD Acinetobacter nosocomialis M2

53.873

97.931

0.528
  pilD Neisseria gonorrhoeae MS11

48.718

94.138

0.459