Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   OU419_RS05065 Genome accession   NZ_CP113432
Coordinates   1063546..1065249 (-) Length   567 a.a.
NCBI ID   WP_254471084.1    Uniprot ID   -
Organism   Pseudomonas triclosanedens strain ZM23     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1058546..1070249
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OU419_RS05030 (OU419_05030) - 1059055..1059522 (+) 468 WP_408004922.1 FAD/FMN-containing dehydrogenase -
  OU419_RS05035 (OU419_05035) - 1059519..1060208 (+) 690 WP_254471090.1 energy-coupling factor ABC transporter permease -
  OU419_RS05040 (OU419_05040) - 1060315..1060530 (+) 216 WP_254471089.1 hypothetical protein -
  OU419_RS05045 (OU419_05045) yacG 1060571..1060777 (-) 207 WP_254471088.1 DNA gyrase inhibitor YacG -
  OU419_RS05050 (OU419_05050) coaE 1060774..1061382 (-) 609 WP_254471087.1 dephospho-CoA kinase -
  OU419_RS05055 (OU419_05055) pilD 1061449..1062321 (-) 873 WP_254471086.1 A24 family peptidase Machinery gene
  OU419_RS05060 (OU419_05060) pilC 1062325..1063542 (-) 1218 WP_254471085.1 type II secretion system F family protein Machinery gene
  OU419_RS05065 (OU419_05065) pilB 1063546..1065249 (-) 1704 WP_254471084.1 type IV-A pilus assembly ATPase PilB Machinery gene
  OU419_RS05070 (OU419_05070) - 1065477..1065953 (+) 477 WP_254471083.1 pilin -
  OU419_RS05075 (OU419_05075) - 1066031..1067758 (+) 1728 WP_254471082.1 phosphoethanolamine transferase -
  OU419_RS05080 (OU419_05080) - 1067728..1068339 (+) 612 WP_254471081.1 fused MFS/spermidine synthase -
  OU419_RS05085 (OU419_05085) - 1068354..1069847 (+) 1494 WP_254471080.1 hypothetical protein -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62517.53 Da        Isoelectric Point: 5.5899

>NTDB_id=761949 OU419_RS05065 WP_254471084.1 1063546..1065249(-) (pilB) [Pseudomonas triclosanedens strain ZM23]
MNDQVVLSGLARQLVQSQLLDEKSAQQAQLQAQRNKLSLVTYLVQNKLVKSRPLTELAAEQFGVAFCDLSSLDRESIPKE
LVSEKLIRQHRVLPLWRRGNKLFVGLSDPTNHQAITDVQFSTGLTTEAILVEDDKLGDAIEKVFESASGGLDDLGDVDLD
GLDIESGDAEPKEDVGGDSADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKIYRVRFRTDGMLHEVAKPPIQLASRISAR
LKVMAGLDISERRKPQDGRIKMRVSKSKSIDFRVNTMPTLWGEKIVMRILDSSSAQMGIDALGYEESQKQLYLEALKQPQ
GMILVTGPTGSGKTVSLYTGINILNTPDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVAAFNLATSVNLIIAQRLARKLCPHCKKEHDVPRETLLHEGFP
ESAIGTFKLYAPVGCENCKGGYKGRVGIYEVVKNTPPLQRIIMEEGNSIQIAEQARKEGFNDLRTSGLMKAMQGITSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=761949 OU419_RS05065 WP_254471084.1 1063546..1065249(-) (pilB) [Pseudomonas triclosanedens strain ZM23]
ATGAACGATCAAGTCGTCCTGTCCGGCCTTGCCCGCCAGTTGGTGCAATCGCAACTGCTCGATGAAAAGAGTGCGCAACA
GGCCCAATTGCAGGCCCAACGCAACAAGCTGTCGCTGGTGACCTACCTGGTCCAGAACAAACTGGTGAAGAGCCGGCCGC
TGACAGAGCTTGCAGCCGAACAGTTCGGCGTTGCCTTCTGCGACCTGAGCAGTCTGGACCGGGAAAGCATCCCCAAGGAG
CTGGTCAGCGAAAAGCTGATTCGCCAGCACCGGGTCCTGCCGCTGTGGCGCCGGGGCAACAAGTTGTTCGTCGGCCTGTC
CGACCCGACCAACCACCAGGCCATCACCGACGTTCAGTTCAGTACGGGGCTGACCACCGAAGCGATCCTGGTCGAAGATG
ACAAGCTCGGCGATGCCATCGAGAAGGTCTTTGAAAGTGCTTCGGGCGGACTCGACGATCTAGGCGACGTCGATCTGGAC
GGCCTGGACATCGAAAGCGGCGACGCCGAGCCCAAGGAGGATGTCGGCGGCGATAGCGCCGATGACGCTCCGGTGGTGCG
CTTCGTCAACAAGATGCTGCTCGATGCAATCAAGGGCGGCTCTTCCGACCTCCACTTCGAACCCTACGAGAAGATCTACC
GTGTGCGCTTCCGTACCGACGGCATGCTGCACGAGGTCGCCAAGCCGCCGATCCAGTTGGCCAGCCGAATCTCCGCGCGC
CTGAAGGTCATGGCGGGCCTCGACATCTCCGAGCGCCGCAAGCCCCAGGATGGCCGGATCAAGATGCGCGTCTCGAAGAG
CAAATCCATCGACTTCCGCGTCAATACCATGCCCACGTTGTGGGGCGAGAAGATCGTGATGCGGATCCTCGACTCCTCCA
GCGCACAAATGGGGATCGATGCGCTGGGTTATGAAGAGAGCCAGAAGCAGCTCTACCTCGAAGCGCTGAAACAGCCGCAA
GGCATGATCCTGGTGACCGGCCCGACCGGTTCGGGCAAGACCGTTTCGCTCTACACAGGCATCAACATCCTCAACACCCC
CGACATCAACATTTCCACTGCCGAAGACCCCGTCGAGATCAACCTCGAGGGCATCAACCAGGTGAACGTCAATCCGCGTC
AGGGCATGGACTTCTCCCAGGCGCTGCGTGCGTTCCTGCGCCAGGACCCGGACGTCATCATGGTCGGTGAGATCCGTGAC
CTGGAGACCGCCGAAATCGCGATCAAAGCAGCGCAGACAGGGCACATGGTGATGTCCACGCTGCACACCAACAGCGCCGC
GGAAACGCTCACGCGCCTGCTGAACATGGGTGTCGCCGCGTTCAACCTGGCCACTTCGGTCAACCTCATCATCGCCCAGC
GTCTGGCACGCAAACTCTGCCCGCACTGCAAGAAAGAACACGACGTGCCACGGGAAACATTACTGCATGAAGGTTTCCCG
GAAAGCGCTATCGGCACATTCAAGCTTTATGCGCCGGTTGGATGTGAAAACTGCAAGGGCGGCTATAAAGGTCGAGTGGG
TATTTATGAAGTGGTTAAAAACACTCCCCCACTGCAGCGAATTATCATGGAAGAAGGCAACTCCATTCAGATCGCGGAAC
AAGCCCGCAAAGAAGGCTTCAATGATCTGCGCACTTCGGGCCTGATGAAAGCCATGCAAGGCATTACCAGCCTCGAAGAA
GTCAACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.848

100

0.578
  pilB Acinetobacter baylyi ADP1

57.194

99.295

0.568
  pilB Legionella pneumophila strain ERS1305867

55.556

100

0.556
  pilB Vibrio parahaemolyticus RIMD 2210633

50.887

99.471

0.506
  pilB Vibrio cholerae strain A1552

50.532

99.471

0.503
  pilB Vibrio campbellii strain DS40M4

50

99.824

0.499
  pilF Neisseria gonorrhoeae MS11

49.559

100

0.496
  pilF Thermus thermophilus HB27

38.214

98.765

0.377
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.171

93.651

0.367
  pilB/pilB1 Synechocystis sp. PCC 6803

39.808

91.711

0.365