Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   OSV15_RS05175 Genome accession   NZ_CP113257
Coordinates   1075384..1077087 (+) Length   567 a.a.
NCBI ID   WP_267932281.1    Uniprot ID   A0AA47E4X7
Organism   Stutzerimonas frequens strain TF18     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1070384..1082087
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OSV15_RS05160 (OSV15_05160) cysD 1071174..1072091 (+) 918 WP_110777989.1 sulfate adenylyltransferase subunit CysD -
  OSV15_RS05165 (OSV15_05165) cysN 1072104..1074002 (+) 1899 WP_267932280.1 sulfate adenylyltransferase subunit CysN -
  OSV15_RS05170 (OSV15_05170) pilA 1074591..1075073 (-) 483 WP_042928458.1 pilin Machinery gene
  OSV15_RS05175 (OSV15_05175) pilB 1075384..1077087 (+) 1704 WP_267932281.1 type IV-A pilus assembly ATPase PilB Machinery gene
  OSV15_RS05180 (OSV15_05180) pilC 1077089..1078306 (+) 1218 WP_267932282.1 type II secretion system F family protein Machinery gene
  OSV15_RS05185 (OSV15_05185) pilD 1078309..1079178 (+) 870 WP_267932283.1 prepilin peptidase Machinery gene
  OSV15_RS05190 (OSV15_05190) coaE 1079311..1079919 (+) 609 WP_267932284.1 dephospho-CoA kinase -
  OSV15_RS05195 (OSV15_05195) yacG 1079916..1080113 (+) 198 WP_102840588.1 DNA gyrase inhibitor YacG -
  OSV15_RS05200 (OSV15_05200) - 1080138..1080818 (-) 681 WP_102840589.1 energy-coupling factor ABC transporter permease -
  OSV15_RS05205 (OSV15_05205) - 1080857..1081894 (-) 1038 WP_267932285.1 NADP(H)-dependent aldo-keto reductase -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 61888.82 Da        Isoelectric Point: 5.2370

>NTDB_id=761305 OSV15_RS05175 WP_267932281.1 1075384..1077087(+) (pilB) [Stutzerimonas frequens strain TF18]
MSENTSLSGLARQMVAAGVLDEKTAQQAQTQAIRGKTPFVTYVVQNRLAKGRVIAELASEQFGVALLDLAALDKESLPKD
LVSEKLAKQHRVLPLSKRGTKLYVAVSDPANHQSIADIQFSTGLSIEPILVEDDKLGEAIDKLYDSGATGLEDLGEVDLD
GLDVETVSDDGRADDAGEAADDAPVVRFVNKMLLDAIRGGSSDLHFEPYERAYRVRFRTDGILHEIARPPIQLAPRISAR
LKVMAGLDISERRKPQDGRIKMKLSKSKAIDFRVNTLPTLWGEKIVMRILDPSSAQMGIDALGYEEEQKALYMQALQQPQ
GMILVTGPTGSGKTVSLYTGLNILNTVDVNISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDIIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVAAFNIATSVNLIIAQRLARKLCNSCKKEIDLPREALLEEGFP
ADKIGTFKIYGPVGCDNCKGGYKGRVGIYEVVKNTPALARIIMEDGNSIDISTQMRKDGFNDLRTSALLKAMQGVTSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=761305 OSV15_RS05175 WP_267932281.1 1075384..1077087(+) (pilB) [Stutzerimonas frequens strain TF18]
ATGAGCGAGAATACTTCCCTTTCCGGGCTGGCGCGGCAGATGGTTGCGGCTGGTGTGCTGGACGAGAAGACGGCGCAGCA
GGCCCAGACTCAGGCCATTCGCGGCAAGACGCCCTTTGTCACCTACGTCGTCCAAAACCGGTTGGCCAAGGGCCGTGTCA
TTGCCGAACTAGCCTCCGAGCAGTTCGGTGTTGCGCTGCTGGATCTTGCTGCGCTGGATAAGGAGTCGCTGCCTAAAGAT
CTGGTGAGCGAGAAGCTGGCCAAGCAGCACCGGGTCCTTCCCCTGTCAAAGCGCGGCACCAAGCTGTATGTGGCGGTGTC
CGACCCTGCCAACCATCAATCGATTGCTGATATCCAGTTCAGCACCGGGCTTTCGATCGAGCCGATTCTGGTCGAGGACG
ACAAACTCGGGGAGGCGATCGACAAGCTCTACGATAGCGGTGCAACCGGCCTGGAGGACCTGGGAGAGGTCGATCTCGAT
GGGCTGGATGTCGAGACCGTTAGCGACGACGGTCGGGCGGATGATGCAGGGGAGGCCGCTGATGATGCCCCGGTCGTGCG
CTTCGTCAACAAGATGTTGCTCGATGCCATTCGTGGTGGTTCCTCCGACTTGCATTTTGAACCCTATGAAAGAGCCTATC
GCGTCCGGTTCCGCACCGATGGGATTCTGCACGAGATCGCTCGTCCCCCGATCCAGTTGGCGCCTCGCATATCGGCCCGT
CTGAAAGTGATGGCAGGGTTGGATATATCCGAGCGGCGTAAACCCCAGGACGGTCGGATCAAGATGAAGCTGTCCAAGTC
CAAGGCGATCGATTTTCGGGTCAATACCTTGCCTACGCTCTGGGGCGAGAAGATCGTGATGCGAATCCTCGATCCGTCCA
GTGCGCAGATGGGGATCGATGCGCTGGGGTACGAGGAAGAACAGAAGGCGCTCTATATGCAGGCGCTTCAGCAGCCACAG
GGCATGATACTGGTAACCGGCCCGACGGGTTCGGGCAAAACGGTATCCCTTTATACCGGTCTCAATATCCTCAATACGGT
CGACGTAAATATCTCCACCGCTGAGGATCCGGTGGAAATCAACCTGGAAGGTATCAACCAGGTCAACGTCAATCCCCGGC
AGGGGATGGACTTCTCCCAGGCGCTGCGCGCATTTCTACGGCAGGACCCCGATATCATCATGGTGGGTGAGATTCGGGAC
TTGGAAACCGCCGAGATCGCCATCAAGGCCGCGCAGACCGGGCACATGGTTATGTCGACGCTGCATACCAACAGCGCCGC
AGAAACCCTTACGCGCCTTCGCAACATGGGGGTGGCAGCGTTCAATATCGCTACGTCGGTGAACCTGATCATTGCTCAGC
GACTGGCGCGCAAGCTGTGTAACAGTTGCAAGAAAGAGATCGATCTGCCGCGCGAGGCTCTGTTGGAAGAGGGCTTCCCC
GCCGACAAGATTGGAACCTTCAAGATCTATGGTCCGGTTGGTTGCGACAATTGCAAAGGCGGTTACAAAGGTCGTGTCGG
TATTTATGAAGTGGTTAAAAACACGCCAGCGTTGGCCAGGATTATCATGGAAGACGGTAATTCGATCGATATTTCCACCC
AGATGCGAAAAGACGGCTTCAATGACCTGCGCACCTCGGCTTTGCTCAAGGCCATGCAGGGCGTGACCAGCCTGGAAGAA
GTCAACCGGGTGACCAAGGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(15-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

60.106

99.471

0.598
  pilB Acinetobacter baylyi ADP1

59.929

99.471

0.596
  pilB Legionella pneumophila strain ERS1305867

55.769

100

0.563
  pilB Vibrio cholerae strain A1552

50.178

99.118

0.497
  pilF Neisseria gonorrhoeae MS11

48.668

99.295

0.483
  pilB Vibrio campbellii strain DS40M4

47.902

100

0.483
  pilB Vibrio parahaemolyticus RIMD 2210633

51.823

91.887

0.476
  pilB/pilB1 Synechocystis sp. PCC 6803

39.272

92.063

0.362