Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   OUY06_RS07265 Genome accession   NZ_CP113247
Coordinates   1432106..1432996 (-) Length   296 a.a.
NCBI ID   WP_076645272.1    Uniprot ID   -
Organism   Latilactobacillus sakei strain CNSC001WB     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1427106..1437996
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OUY06_RS07230 - 1428172..1429182 (-) 1011 WP_076645260.1 class I SAM-dependent methyltransferase -
  OUY06_RS07235 - 1429306..1429644 (-) 339 WP_076645262.1 hypothetical protein -
  OUY06_RS07240 comGF 1429607..1430119 (-) 513 WP_025016386.1 competence type IV pilus minor pilin ComGF Machinery gene
  OUY06_RS07245 comGE 1430094..1430399 (-) 306 WP_076645264.1 hypothetical protein Machinery gene
  OUY06_RS07250 comGD 1430386..1430838 (-) 453 WP_278319739.1 competence type IV pilus minor pilin ComGD Machinery gene
  OUY06_RS07255 comGC 1430810..1431109 (-) 300 WP_076645268.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  OUY06_RS07260 comGB 1431106..1432113 (-) 1008 WP_076645270.1 type II secretion system F family protein Machinery gene
  OUY06_RS07265 comGA 1432106..1432996 (-) 891 WP_076645272.1 competence type IV pilus ATPase ComGA Machinery gene
  OUY06_RS07270 - 1433112..1433843 (-) 732 WP_011375002.1 YebC/PmpR family DNA-binding transcriptional regulator -
  OUY06_RS07275 - 1433934..1434434 (-) 501 WP_076632058.1 VanZ family protein -
  OUY06_RS07280 - 1434531..1434905 (+) 375 WP_011375004.1 hypothetical protein -
  OUY06_RS07290 - 1435092..1436489 (-) 1398 WP_076645274.1 hypothetical protein -
  OUY06_RS07295 - 1436503..1437558 (-) 1056 WP_143455104.1 hypothetical protein -

Sequence

Protein


Download         Length: 296 a.a.        Molecular weight: 32973.86 Da        Isoelectric Point: 6.3848

>NTDB_id=761186 OUY06_RS07265 WP_076645272.1 1432106..1432996(-) (comGA) [Latilactobacillus sakei strain CNSC001WB]
MSTEEKLTLLLKHCAKQHVQDLYFTPTIDGWRLTERRQSDLVTNQELDKPTGTRYLNRLKYMAGMDISETRRAQTGRSEL
QLADQTIYLRLATVGDFLNRESLVIRFIYPIGAIYHCDDQQILAQLTQMSRKAGLILFAGPTGSGKTTSLYHLAQQSMSA
KMVVAIEDPIEIVAPEFLQLQVNDNAGLSYAELLKISLRLRPDTLIIGEIRDLETAQYAVSAALSGHLVLSTIHARSTQG
VVARLLDLGISELQLRACLTGIAYQSLVTKDDVVQAHYELLTGPDYFSKKEGGCDV

Nucleotide


Download         Length: 891 bp        

>NTDB_id=761186 OUY06_RS07265 WP_076645272.1 1432106..1432996(-) (comGA) [Latilactobacillus sakei strain CNSC001WB]
ATGTCAACTGAAGAAAAGCTAACTTTATTACTAAAACATTGTGCTAAGCAGCATGTGCAAGATCTCTATTTTACACCGAC
AATAGATGGCTGGCGCTTGACGGAGCGTCGTCAAAGCGATTTAGTTACCAATCAAGAATTGGATAAACCGACTGGAACGC
GTTATTTAAATCGACTGAAGTATATGGCGGGGATGGATATCAGCGAGACTCGGCGTGCGCAAACCGGCCGCTCGGAATTA
CAGCTAGCCGACCAAACAATTTATTTACGCCTAGCGACGGTCGGGGATTTTTTAAATCGCGAAAGCTTGGTCATTCGGTT
TATCTATCCAATTGGTGCCATCTATCATTGTGATGATCAACAGATATTGGCGCAATTAACGCAGATGAGTCGAAAGGCGG
GCTTAATTCTTTTCGCTGGGCCGACTGGTTCTGGCAAAACGACTAGCCTCTATCATCTGGCCCAACAATCAATGAGTGCC
AAGATGGTGGTTGCTATTGAAGATCCGATTGAAATTGTTGCACCTGAGTTTTTGCAACTGCAGGTTAATGATAATGCGGG
ATTGAGCTACGCCGAGCTATTAAAAATTAGTTTACGACTGCGACCGGATACGCTGATTATCGGTGAAATTCGCGATTTAG
AGACAGCTCAATATGCAGTTTCTGCGGCCTTAAGTGGCCACCTGGTATTGAGTACTATTCACGCTCGGAGTACACAGGGC
GTGGTCGCGCGCTTATTAGATCTTGGTATTTCTGAGCTGCAATTGCGGGCCTGTCTAACGGGAATTGCCTACCAATCGTT
GGTGACAAAAGACGACGTTGTGCAAGCCCATTATGAATTATTAACTGGTCCAGATTATTTCTCAAAAAAAGAAGGTGGTT
GTGATGTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Latilactobacillus sakei subsp. sakei 23K

97.635

100

0.976
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

42.642

89.527

0.382
  comGA Lactococcus lactis subsp. cremoris KW2

37.919

100

0.382
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

40.876

92.568

0.378
  comGA/cglA/cilD Streptococcus pneumoniae D39

42.264

89.527

0.378
  comGA/cglA/cilD Streptococcus pneumoniae R6

42.264

89.527

0.378
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

42.264

89.527

0.378
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

42.264

89.527

0.378
  comYA Streptococcus gordonii str. Challis substr. CH1

40.959

91.554

0.375