Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   P7I78_RS07095 Genome accession   NZ_CP124646
Coordinates   1484285..1485502 (-) Length   405 a.a.
NCBI ID   WP_016254080.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain 2020CK-00185     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1479285..1490502
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P7I78_RS07050 (P7I78_07050) - 1479467..1479916 (-) 450 WP_003141357.1 MOSC domain-containing protein -
  P7I78_RS07055 (P7I78_07055) - 1479913..1480542 (-) 630 WP_003094668.1 DUF1780 domain-containing protein -
  P7I78_RS07060 (P7I78_07060) - 1480675..1481100 (+) 426 WP_003103865.1 GNAT family N-acetyltransferase -
  P7I78_RS07065 (P7I78_07065) - 1481097..1481567 (+) 471 WP_003094664.1 hypothetical protein -
  P7I78_RS07070 (P7I78_07070) - 1481564..1482253 (+) 690 WP_003103868.1 energy-coupling factor ABC transporter permease -
  P7I78_RS07075 (P7I78_07075) - 1482359..1482568 (+) 210 WP_003094660.1 hypothetical protein -
  P7I78_RS07080 (P7I78_07080) yacG 1482605..1482805 (-) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  P7I78_RS07085 (P7I78_07085) coaE 1482802..1483413 (-) 612 WP_003094654.1 dephospho-CoA kinase -
  P7I78_RS07090 (P7I78_07090) pilD 1483410..1484282 (-) 873 WP_003141356.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  P7I78_RS07095 (P7I78_07095) pilC 1484285..1485502 (-) 1218 WP_016254080.1 type II secretion system F family protein Machinery gene
  P7I78_RS07100 (P7I78_07100) - 1485506..1485679 (-) 174 Protein_1405 type IV-A pilus assembly ATPase PilB -
  P7I78_RS07105 (P7I78_07105) - 1485736..1486764 (+) 1029 WP_034023821.1 IS30-like element ISPsp7 family transposase -
  P7I78_RS07110 (P7I78_07110) pilB 1486793..1488325 (-) 1533 Protein_1407 type IV-A pilus assembly ATPase PilB -
  P7I78_RS07115 (P7I78_07115) - 1488554..1488637 (+) 84 Protein_1408 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  P7I78_RS07120 (P7I78_07120) - 1488668..1489696 (-) 1029 WP_034023821.1 IS30-like element ISPsp7 family transposase -
  P7I78_RS07125 (P7I78_07125) - 1489784..1490209 (+) 426 WP_282415605.1 pilin -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44041.92 Da        Isoelectric Point: 9.8417

>NTDB_id=759340 P7I78_RS07095 WP_016254080.1 1484285..1485502(-) (pilC) [Pseudomonas aeruginosa strain 2020CK-00185]
MADKALKTSVFIWEGTDKKGSKVKGELAGQNPMLVKAQLRKQGINPLKVRKKGITLFGAGKKIKPMDIALFTRQMSTMMG
AGVPLLQSFDIIGEGFENPNMRKLVDEIKQDVAAGNSLASSLRKKPIYFDDLYCNLVDAGEQSGALETLLDRVATYKEKT
ESLKAKIKKAMTYPIAVIVVALVVSAILLIKVVPQFQSVFANFGAELPAFTQMVINLSEMLQEWWLIVLIGLFAAAFAFR
EAHRRSQKLRDSVDRGLLKLPIIGGILYKSAIARYARTLSTTFAAGVPLVEALDSVSGATGNVVFKNAVTKIKQDVSSGM
QLNFSMRTTNVFPSMAIQMAAIGEESGSLDEMLGKVAGFYEEEVDNAVDNLTTLMEPMIMAVLGVLVGGLIIAMYLPIFQ
LGSVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=759340 P7I78_RS07095 WP_016254080.1 1484285..1485502(-) (pilC) [Pseudomonas aeruginosa strain 2020CK-00185]
ATGGCGGACAAAGCGTTAAAGACCAGTGTCTTCATCTGGGAAGGCACTGATAAGAAAGGCAGCAAGGTCAAAGGAGAGTT
GGCCGGGCAAAACCCTATGCTGGTGAAGGCTCAACTGCGCAAACAGGGTATCAACCCACTAAAGGTCCGCAAGAAAGGTA
TCACCCTGTTTGGGGCAGGGAAGAAGATTAAGCCCATGGACATCGCCCTGTTCACTCGGCAGATGTCTACCATGATGGGT
GCCGGCGTACCGTTACTGCAATCTTTTGACATCATCGGCGAAGGATTCGAAAATCCAAACATGCGCAAGCTAGTCGATGA
GATCAAGCAGGATGTTGCCGCCGGTAACAGCTTAGCCAGTTCACTTCGAAAGAAACCCATTTACTTCGATGATCTCTACT
GCAACCTGGTCGATGCTGGCGAACAGTCCGGTGCTTTGGAGACATTATTGGATCGGGTAGCAACTTATAAAGAAAAGACA
GAATCCCTGAAAGCCAAAATTAAAAAAGCCATGACTTATCCCATTGCAGTAATTGTAGTGGCCCTTGTGGTATCGGCGAT
CCTTCTGATAAAAGTGGTCCCACAGTTCCAGTCCGTATTTGCAAATTTTGGTGCCGAGTTGCCGGCCTTTACTCAAATGG
TCATCAATCTTTCCGAGATGCTTCAAGAGTGGTGGCTCATAGTGCTTATTGGTCTTTTTGCCGCAGCTTTTGCATTTAGG
GAAGCTCATCGGCGCTCGCAAAAACTGAGAGATTCAGTAGATCGGGGCCTGCTGAAACTACCTATCATCGGCGGGATACT
TTACAAATCAGCTATCGCCCGCTACGCCCGAACGCTATCCACTACCTTTGCGGCTGGAGTGCCTCTGGTAGAAGCTCTGG
ACTCCGTTTCCGGAGCAACTGGCAACGTGGTATTCAAGAATGCCGTTACCAAGATCAAACAAGACGTGTCCAGCGGCATG
CAATTGAACTTTTCCATGCGCACCACCAATGTTTTCCCCAGTATGGCTATCCAGATGGCTGCAATTGGCGAGGAATCAGG
TTCGCTAGATGAGATGTTAGGAAAAGTTGCCGGTTTCTATGAGGAAGAAGTCGATAACGCCGTCGACAACCTGACAACGT
TGATGGAGCCGATGATCATGGCCGTGCTCGGCGTACTGGTCGGCGGCCTGATCATCGCTATGTATCTACCGATCTTCCAA
CTGGGTTCCGTTGTGTAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

79.753

100

0.798
  pilC Acinetobacter baumannii D1279779

60.891

99.753

0.607
  pilC Acinetobacter baylyi ADP1

59.852

100

0.6
  pilC Legionella pneumophila strain ERS1305867

55.303

97.778

0.541
  pilG Neisseria meningitidis 44/76-A

46.269

99.259

0.459
  pilG Neisseria gonorrhoeae MS11

46.02

99.259

0.457
  pilC Vibrio cholerae strain A1552

42.222

100

0.422
  pilC Vibrio campbellii strain DS40M4

40.909

97.778

0.4
  pilC Thermus thermophilus HB27

38.404

99.012

0.38