Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   OSC49_RS02830 Genome accession   NZ_CP112868
Coordinates   646962..648287 (+) Length   441 a.a.
NCBI ID   WP_253511974.1    Uniprot ID   -
Organism   Pseudomonas quebecensis isolate S1Bt3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 641962..653287
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OSC49_RS02810 (OSC49_02810) - 642028..644496 (-) 2469 WP_253510056.1 penicillin-binding protein 1A -
  OSC49_RS02815 (OSC49_02815) pilM 644710..645363 (+) 654 WP_266245549.1 type IV pilus assembly protein PilM -
  OSC49_RS02820 (OSC49_02820) - 645360..645914 (+) 555 WP_253510060.1 PilN domain-containing protein -
  OSC49_RS02825 (OSC49_02825) - 645911..646891 (+) 981 WP_266245551.1 pilus assembly protein PilP -
  OSC49_RS02830 (OSC49_02830) pilQ 646962..648287 (+) 1326 WP_253511974.1 type IV pilus secretin PilQ family protein Machinery gene
  OSC49_RS02835 (OSC49_02835) aroK 648292..648810 (+) 519 WP_034105907.1 shikimate kinase AroK -
  OSC49_RS02840 (OSC49_02840) aroB 648936..650036 (+) 1101 WP_253510064.1 3-dehydroquinate synthase -
  OSC49_RS02845 (OSC49_02845) - 650047..651624 (+) 1578 WP_181080354.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 441 a.a.        Molecular weight: 47856.77 Da        Isoelectric Point: 4.8167

>NTDB_id=758495 OSC49_RS02830 WP_253511974.1 646962..648287(+) (pilQ) [Pseudomonas quebecensis isolate S1Bt3]
MAAAVPNRIDLIQLPPPGGAMAGAYSGDKLTLNFQNIELRAVLQQIADVAGLNLVASDDVQGSITLRLKDVPWDQALELV
LQTKGLDKRIHAGVLLVAPAEELAARELLTLESRRQIANLTPLRRELLQVNYAKAADLARLFQSVTGLEGATDERGSVAV
DERTNNIIAYQTGERLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGARWGGRLNRGNWQAGGIPKPLPDGVEPPE
NPPGSPFVDLGAIAGASGLGVAYITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETARILKGTEIPYQESSSSGATSV
SFKEASLSLEVTPQITPDDRVIMEVKVTKDEPDYLNRLNDVPPIKKNEVNAKVLVKDGETIVIGGVFSNTQSKVVDKVPF
LGDVPYLGRLFRRDVLAEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 1326 bp        

>NTDB_id=758495 OSC49_RS02830 WP_253511974.1 646962..648287(+) (pilQ) [Pseudomonas quebecensis isolate S1Bt3]
ATGGCTGCCGCTGTGCCCAACCGTATAGATCTTATTCAACTGCCGCCGCCGGGTGGGGCCATGGCGGGTGCCTACAGCGG
CGACAAGCTCACGCTCAACTTCCAGAACATAGAGCTGCGCGCGGTATTGCAGCAGATTGCCGATGTGGCCGGCCTTAACC
TGGTCGCCAGCGATGACGTCCAGGGTTCGATCACACTGCGCCTCAAAGATGTGCCCTGGGATCAGGCGCTGGAGCTGGTG
TTACAGACCAAGGGGTTGGATAAGCGCATTCACGCCGGCGTATTGCTGGTAGCGCCCGCCGAGGAATTGGCAGCCCGCGA
ACTGCTGACGCTGGAGTCGCGCAGGCAGATAGCCAACCTCACACCGCTGCGGCGGGAGCTGTTGCAGGTCAATTATGCCA
AGGCAGCCGATCTGGCCAGGCTGTTCCAGTCGGTGACCGGCCTTGAAGGGGCCACCGACGAACGCGGTTCGGTGGCGGTG
GATGAGCGCACCAACAACATCATCGCCTATCAGACCGGCGAGCGGCTCGACGAGCTGCGGCGCATCGTGGCGCAGTTGGA
TATCCCCGTGCGGCAGGTCATGATCGAAGCGCGGATCGTGGAGGCCAACGTCGATTACGACAAAAGCCTGGGAGCACGCT
GGGGCGGACGGCTCAATCGCGGCAACTGGCAGGCCGGTGGCATTCCAAAGCCCCTGCCCGATGGCGTAGAACCGCCGGAA
AATCCGCCTGGCTCACCGTTTGTCGACCTCGGCGCAATAGCCGGCGCGTCCGGCCTGGGTGTGGCTTACATCACCGACAA
TGTGCTGCTGGACCTGGAACTGACGGCCATGGAAAAGACCGGCAACGGCGAGATCGTTTCCCAGCCCAAGGTCGTGACCT
CCGACAAGGAGACCGCGCGCATCCTTAAGGGCACCGAGATTCCGTACCAGGAGTCCAGCTCCAGTGGCGCCACGTCGGTG
TCGTTCAAGGAGGCCTCGCTGTCGTTGGAAGTGACCCCTCAGATCACCCCTGATGACCGGGTGATCATGGAAGTGAAAGT
CACCAAGGATGAGCCGGACTACCTCAACAGGCTCAACGACGTACCACCGATCAAGAAAAACGAGGTCAACGCCAAGGTGC
TGGTCAAGGATGGCGAGACCATTGTGATCGGCGGAGTTTTCTCCAATACCCAAAGCAAAGTGGTAGATAAAGTGCCATTT
TTGGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGCGATGTGCTGGCGGAGAAAAAATCCGAGCTGCTGGTATTCCT
GACTCCGCGTATTATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(53-97)

Bacterial type II/III secretion system short domain (Secretin_N)

(127-192)

Bacterial type II and III secretion system protein (Secretin)

(276-432)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

69.683

100

0.698
  comQ Acinetobacter baylyi ADP1

42.762

100

0.435
  pilQ Acinetobacter baumannii D1279779

41.379

100

0.435
  pilQ Acinetobacter baumannii strain A118

41.379

100

0.435
  pilQ Legionella pneumophila strain Lp02

44.208

95.918

0.424
  pilQ Legionella pneumophila strain ERS1305867

43.81

95.238

0.417
  pilQ Vibrio campbellii strain DS40M4

42.89

97.279

0.417
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

43.78

94.785

0.415
  pilQ Vibrio cholerae strain A1552

43.78

94.785

0.415