Detailed information NTG0075653

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: KI241_RS16940
• Genome accession: NZ_AP021902
• Coordinates: 3902098..3903213 (-)
• Length: 371 a.a.
• NCBI ID: WP_042732331.1
• Uniprot ID: A0A0D0P3H5
• Organism: Pseudomonas sp. Cab53
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3897098..3908213

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KI241_RS16925 (PHLH5_33970)	-	3897581..3898972 (+)	1392	WP_042732360.1	GntP family permease	-
KI241_RS16930 (PHLH5_33980)	-	3898980..3899756 (+)	777	WP_212802772.1	3-hydroxybutyrate dehydrogenase	-
KI241_RS16935 (PHLH5_33990)	-	3899966..3901921 (+)	1956	WP_212802773.1	acetoacetate--CoA ligase	-
KI241_RS16940 (PHLH5_34000)	pilU	3902098..3903213 (-)	1116	WP_042732331.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
KI241_RS16945 (PHLH5_34010)	-	3903346..3905883 (+)	2538	WP_212802774.1	PAS domain-containing protein	-
KI241_RS16950 (PHLH5_34020)	-	3905929..3906204 (+)	276	WP_019690952.1	peptidylprolyl isomerase	-
KI241_RS16955 (PHLH5_34030)	-	3906326..3907243 (-)	918	WP_042731869.1	sugar kinase	-

Sequence

Protein

• Length: 371 a.a.
• Molecular weight: 40973.87 Da
• Isoelectric Point: 6.0174

>NTDB_id=75653 KI241_RS16940 WP_042732331.1 3902098..3903213(-) (pilU) [Pseudomonas sp. Cab53]
MEIDPLLRILASQDGSDLYLSTGAPPCAKFEGVLKPLGSQAFKVGDIAGLAESLMDAEQRLEFDRELEMNLAISLAGVGR
FRVNVFKQRNDVSMVIRNVKLDIPRFEDLKLPPVLLESVMRKQGLILFVGATGSGKSTSLAALIDYRNRHSSGHIITIED
PVEYIHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALAFADTGHLVISTLHAHNANQALDRV
INFFPEERRPQLLSDLANNLQAFISQRLVRTVSGQRRAAVEVMLGSPTVADLIRRNELSELKGIMEKSEELGMQTFDQAL
FNLVVEGAIDEEQALKNADSANNLRLRLKLHAESGAAPPADPAAGEWGLVD

Nucleotide

• Length: 1116 bp

>NTDB_id=75653 KI241_RS16940 WP_042732331.1 3902098..3903213(-) (pilU) [Pseudomonas sp. Cab53]
ATGGAAATCGATCCCTTGTTGCGAATCCTGGCGAGCCAGGATGGCTCCGACCTTTATCTGTCCACCGGTGCACCGCCGTG
CGCGAAATTCGAGGGGGTGCTCAAGCCTCTGGGTTCCCAGGCGTTCAAGGTTGGCGACATTGCCGGGCTCGCCGAGTCTT
TGATGGACGCCGAACAGCGCCTTGAGTTCGATCGAGAGCTGGAAATGAACCTGGCAATTTCCCTCGCCGGCGTCGGGCGG
TTCCGGGTCAACGTCTTCAAGCAGCGTAACGACGTGTCCATGGTGATCCGCAACGTCAAGCTGGACATCCCGCGCTTCGA
AGACCTGAAGCTGCCTCCGGTGCTGCTGGAGAGCGTGATGCGCAAACAGGGGCTGATCCTGTTCGTCGGCGCCACGGGGT
CCGGCAAGTCGACGTCCCTGGCGGCGTTGATCGATTACCGCAATCGCCACAGCAGTGGCCACATCATCACCATCGAGGAC
CCGGTGGAGTATATCCATCGGCACAAAAAATCGATCATCAACCAGCGTGAGGTCGGTGTCGACACCCGCAGCTTTCACGC
CGCCCTGAAAAATACCCTGCGCCAGGCGCCGGATGTGGTGCTGATCGGTGAAATCCGCGACCGGGAAACCATGGAACACG
CCCTGGCCTTCGCCGACACCGGGCACCTGGTGATTTCCACGTTGCATGCCCATAACGCCAACCAGGCCCTGGACCGGGTG
ATCAATTTCTTTCCCGAAGAGCGCCGGCCGCAACTGCTCAGCGACCTGGCCAACAACCTCCAGGCCTTCATCTCCCAGCG
CCTGGTGCGCACCGTGAGCGGCCAGCGCCGCGCGGCGGTGGAGGTGATGCTGGGCTCGCCCACGGTGGCCGACCTGATCC
GGCGCAACGAACTGAGCGAGCTCAAGGGCATCATGGAAAAATCGGAAGAGCTGGGCATGCAAACCTTCGATCAGGCCCTG
TTCAACCTGGTGGTGGAAGGCGCCATCGACGAGGAGCAGGCGCTGAAGAATGCCGACTCGGCGAACAACCTGCGCTTGCG
CCTGAAGCTGCATGCCGAGTCGGGCGCCGCGCCGCCGGCTGATCCGGCGGCGGGGGAGTGGGGGTTGGTGGATTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (80-271)

3D structure

• AlphaFold DB: A0A0D0P3H5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.056	95.687	0.536
pilU	Acinetobacter baylyi ADP1	53.168	97.844	0.52
pilU	Vibrio cholerae strain A1552	52.857	94.34	0.499
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	38.965	98.922	0.385
pilT	Pseudomonas stutzeri DSM 10701	40.407	92.722	0.375
pilT	Vibrio cholerae strain A1552	41.441	89.757	0.372
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	41.441	89.757	0.372
pilT	Pseudomonas aeruginosa PAK	39.826	92.722	0.369