Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   OPR71_RS02895 Genome accession   NZ_CP110670
Coordinates   599466..601151 (-) Length   561 a.a.
NCBI ID   WP_267923005.1    Uniprot ID   -
Organism   Vibrio alginolyticus strain AUSMDU00064140     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 594466..606151
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OPR71_RS02870 (OPR71_02870) yacG 595549..595743 (-) 195 WP_017635922.1 DNA gyrase inhibitor YacG -
  OPR71_RS02875 (OPR71_02875) zapD 595902..596642 (-) 741 WP_267923003.1 cell division protein ZapD -
  OPR71_RS02880 (OPR71_02880) coaE 596670..597284 (-) 615 WP_017635920.1 dephospho-CoA kinase -
  OPR71_RS02885 (OPR71_02885) pilD 597285..598154 (-) 870 WP_158174153.1 prepilin peptidase Machinery gene
  OPR71_RS02890 (OPR71_02890) pilC 598219..599442 (-) 1224 WP_017820393.1 type II secretion system F family protein Machinery gene
  OPR71_RS02895 (OPR71_02895) pilB 599466..601151 (-) 1686 WP_267923005.1 type IV-A pilus assembly ATPase PilB Machinery gene
  OPR71_RS02900 (OPR71_02900) - 601151..601585 (-) 435 WP_029803401.1 type IV pilin protein -
  OPR71_RS02910 (OPR71_02910) nadC 601849..602736 (-) 888 WP_005379983.1 carboxylating nicotinate-nucleotide diphosphorylase -
  OPR71_RS02915 (OPR71_02915) ampD 602829..603380 (+) 552 WP_005379985.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  OPR71_RS02920 (OPR71_02920) pdhR 603786..604553 (+) 768 WP_005379989.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62103.91 Da        Isoelectric Point: 5.5375

>NTDB_id=755906 OPR71_RS02895 WP_267923005.1 599466..601151(-) (pilB) [Vibrio alginolyticus strain AUSMDU00064140]
MQTNLSTILRQKGLLAFSQEESLLEQVKASGISMPEALLSSGLFTSSELAEHLSSLFGLNQPELSQYEYASLCQQLGLRE
LITRHNALPLQRTSSTLLLAVADPTNQQAEDDFRFATGLQVELVLGEFSQLSAAIRRLYGRSLSHEKSGLKEINQEELAN
LVDVGADEIDNIEDLSQDESPVSRYINQILLDAVRKGASDIHFEPYEKMYRVRLRCDGILIETQQPPSHLSRRLSARIKI
LSKLDIAERRLPQDGRIKLKLNQDTAIDMRVSTLPTLFGEKIVLRLLDSSSASLDIDKLGYSEQQKQLYLDALRRPQGMI
LMTGPTGSGKTVSLYTGLSILNKPEINISTAEDPVEINLSGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLDT
AEIAIKASQTGHLVLSTLHTNSAAETVIRLSNMGVESFNLASSLSLIIAQRLARKLCSHCKKSQELTVQLQHLGIQASDN
IFKANPDGCNECTHGYSGRTGIYEVMRFDEFLSEALIKGASVHELEKLAIANGMSTLQMSGIEKLKQGITSFSELQRVLY
F

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=755906 OPR71_RS02895 WP_267923005.1 599466..601151(-) (pilB) [Vibrio alginolyticus strain AUSMDU00064140]
ATGCAAACCAACCTCTCCACGATTCTTCGTCAAAAAGGACTATTGGCTTTTTCCCAAGAAGAATCGTTGCTTGAGCAAGT
CAAAGCTTCTGGCATTTCGATGCCAGAGGCTTTACTCAGTTCTGGATTATTTACATCAAGCGAACTCGCTGAGCACTTAA
GTTCTCTCTTTGGTTTAAATCAGCCCGAGTTATCTCAGTACGAGTATGCTTCGCTTTGTCAGCAACTTGGCCTTAGGGAA
TTAATCACGCGGCATAACGCACTCCCACTCCAGCGTACGTCTTCAACGTTATTACTCGCCGTTGCCGACCCCACCAACCA
ACAAGCAGAAGATGATTTTCGCTTTGCCACTGGTTTACAGGTTGAATTGGTCTTGGGGGAATTTAGTCAACTCAGCGCGG
CAATTCGACGCTTGTATGGTCGTTCATTGAGTCATGAGAAGTCCGGGTTAAAAGAGATCAACCAAGAAGAGTTAGCCAAT
CTGGTTGACGTGGGCGCAGATGAAATCGACAACATTGAAGACTTGAGCCAAGATGAGTCTCCTGTTAGCCGTTACATCAA
CCAAATTCTATTGGACGCTGTGCGCAAAGGCGCATCCGATATCCACTTTGAGCCTTATGAAAAGATGTACCGCGTGCGCC
TGCGCTGTGATGGCATCTTAATTGAAACACAGCAGCCACCAAGCCACTTGAGTCGTCGCTTATCCGCTCGTATCAAAATC
CTCTCTAAGCTTGATATTGCCGAAAGGCGCTTACCACAAGATGGGCGTATTAAGCTCAAGTTGAATCAAGACACCGCAAT
TGATATGCGTGTATCCACGCTACCGACTCTATTCGGAGAGAAAATTGTACTACGACTGCTCGACAGCAGCTCTGCGTCAT
TGGATATCGACAAGCTTGGTTATAGCGAACAACAAAAACAATTGTACTTGGATGCGCTGCGTCGCCCACAAGGCATGATT
CTGATGACAGGTCCAACCGGGAGCGGAAAAACCGTTTCTCTGTACACTGGGCTGAGTATCCTCAATAAGCCAGAAATCAA
CATTTCCACGGCAGAAGACCCAGTAGAAATTAACTTATCGGGCATTAACCAAGTTCAAGTTCAGCCTAAGATTGGTTTTG
GTTTTGCCGAAGCACTGCGTTCTTTCTTACGTCAAGACCCCGATGTGGTAATGGTCGGTGAGATTCGTGACTTAGATACC
GCCGAAATCGCTATAAAAGCATCGCAAACCGGTCACTTAGTACTTTCAACCTTACATACCAATTCTGCGGCTGAAACGGT
TATTCGTTTATCAAACATGGGCGTAGAAAGCTTTAACTTGGCCTCCTCTCTTAGTTTGATCATTGCCCAGCGCTTGGCCC
GTAAACTGTGCTCGCACTGTAAAAAATCACAAGAGCTTACCGTTCAGCTACAACATCTTGGAATTCAAGCAAGCGACAAC
ATTTTTAAAGCCAATCCCGATGGATGTAATGAGTGTACCCATGGTTATTCCGGCCGAACGGGGATTTATGAAGTGATGCG
CTTTGATGAATTTCTTTCCGAAGCGCTTATTAAAGGCGCCTCGGTGCATGAACTAGAAAAACTCGCCATCGCAAACGGGA
TGAGCACGTTACAAATGTCGGGCATAGAAAAACTCAAACAAGGGATCACAAGTTTTAGTGAACTACAGCGCGTACTTTAT
TTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-144)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio parahaemolyticus RIMD 2210633

95.009

100

0.95
  pilB Vibrio campbellii strain DS40M4

89.483

100

0.895
  pilB Vibrio cholerae strain A1552

74.021

100

0.742
  pilB Legionella pneumophila strain ERS1305867

48.746

99.465

0.485
  pilB Acinetobacter baumannii D1279779

51.81

93.583

0.485
  pilB Acinetobacter baylyi ADP1

47.935

99.287

0.476
  pilF Neisseria gonorrhoeae MS11

44.858

100

0.451
  pilB/pilB1 Synechocystis sp. PCC 6803

37.416

100

0.398
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.508

100

0.369
  pilF Thermus thermophilus HB27

36.299

100

0.364