Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ONZ66_RS02030 Genome accession   NZ_CP110647
Coordinates   408428..410134 (-) Length   568 a.a.
NCBI ID   WP_271774096.1    Uniprot ID   -
Organism   Aeromonas salmonicida strain ZAS     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 403428..415134
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ONZ66_RS02000 (ONZ66_02000) mutT 404036..404443 (+) 408 WP_099994491.1 8-oxo-dGTP diphosphatase MutT -
  ONZ66_RS02005 (ONZ66_02005) yacG 404521..404715 (-) 195 WP_059113458.1 DNA gyrase inhibitor YacG -
  ONZ66_RS02010 (ONZ66_02010) zapD 404725..405447 (-) 723 WP_021140336.1 cell division protein ZapD -
  ONZ66_RS02015 (ONZ66_02015) coaE 405485..406099 (-) 615 WP_271774095.1 dephospho-CoA kinase -
  ONZ66_RS02020 (ONZ66_02020) pilD 406119..406994 (-) 876 WP_125729778.1 prepilin peptidase Machinery gene
  ONZ66_RS02025 (ONZ66_02025) pilC 407058..408299 (-) 1242 WP_059113460.1 type II secretion system F family protein Machinery gene
  ONZ66_RS02030 (ONZ66_02030) pilB 408428..410134 (-) 1707 WP_271774096.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  ONZ66_RS02035 (ONZ66_02035) - 410137..410574 (-) 438 WP_271774097.1 pilin -
  ONZ66_RS02040 (ONZ66_02040) nadC 410910..411767 (-) 858 WP_271774098.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ONZ66_RS02045 (ONZ66_02045) - 411772..412242 (-) 471 WP_011898292.1 retropepsin-like aspartic protease family protein -
  ONZ66_RS02050 (ONZ66_02050) ampD 412392..412964 (+) 573 WP_271774954.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ONZ66_RS02055 (ONZ66_02055) pdhR 413241..414005 (+) 765 WP_005314150.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62577.00 Da        Isoelectric Point: 5.4384

>NTDB_id=755643 ONZ66_RS02030 WP_271774096.1 408428..410134(-) (pilB) [Aeromonas salmonicida strain ZAS]
MTSSPNSGLALSLAASSLLSESDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTAAIGKLLESEQDALGMEEIDESEI
SELEVSDENSRLDESVNTTDDDAPIVKYINKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEIATPPVNLANRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
QQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRVSGLEKARLGVTSLA
EINRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=755643 ONZ66_RS02030 WP_271774096.1 408428..410134(-) (pilB) [Aeromonas salmonicida strain ZAS]
ATGACCTCTAGCCCCAATAGCGGCCTGGCGTTAAGCCTGGCCGCCTCATCCCTGCTCAGTGAGTCCGACTCACAGCGTTA
TCTGAGCCAAGCCAAGGCCCAGCGCAAACCCTTCGTGACATTTCTGATTGAAAACGAGATCCTCGACAGCAAGGCGCTGG
CGGATTTTTGCGAGCTGGAATATGGGGTACCTCTGCTGGATCTTGCCGCTTTCGACCTGGCCGAGATCCCGCAGAAGTAC
CTCAATCAGAAGCTGATTGAAAAACACCATGTGCTGCCCATCTACACCCAGGGCCACACCCTCTATATCGCCATGTCGGA
TCCGACCAACGTGTCGGCACTGGAGGATTTTGGCTTCAGCTTCGGGCTCCATACAGAAGCCCTGCTGGTGGAAGAGAACA
AGCTCACCGCCGCCATCGGCAAGCTGCTGGAAAGCGAGCAGGATGCCCTCGGCATGGAGGAGATCGACGAGTCGGAAATC
TCCGAGCTGGAAGTCTCGGACGAAAACTCCCGCCTCGATGAGAGCGTCAATACCACCGACGACGATGCCCCCATCGTCAA
ATACATCAACAAAATCATGATGGATGCCATCAAGCGCGGCGCCTCGGATCTGCACTTCGAACCCTATGAGACCAAGTACC
GTATCCGCTTTCGTATAGACGGCATACTGCACGAAATAGCGACGCCGCCGGTCAATCTGGCCAACCGCTTTTCGGCCCGC
CTCAAGGTGATGGCGCGACTCGATATCGCAGAGCGACGGCTGCCCCAGGATGGTCGCATCAAGTTAAAGCTGTCGCGCAA
CAAATCCATGGATATGCGGGTCAACACACTGCCCACCATGTGGGGCGAGAAGATCGTTATCCGTCTGCTGGACTCTTCCG
CCGCGCGACTCAATATCGAACAGCTCGGCTTCGACGACCGGCAGAAGGCACAGTATCTGCGTGCCCTCTCCAAGCCCCAG
GGCATGATATTGGTGACAGGCCCGACCGGCTCTGGCAAGACTGTCTCTCTCTATACCGGCCTCAATATTCTCAACACCAC
AGAGGTCAACATCTCTACCGCCGAAGATCCGGTGGAGATCAACCTGCCCGGCGTCAACCAGGTACAAGTCAATCCCAAGG
CGGGGCTGACCTTCGCCAGCGCCCTGCGCTCCTTTCTTCGCCAGGATCCCGATGTGGTGATGGTGGGAGAAATCCGGGAT
CTGGAAACCGCCGAGATTGCCATCAAAGCCGCCCAGACCGGCCACCTGGTGCTCTCCACCCTGCACACCAACTCGGCTGC
CGAAACCCTGACCCGGATGATGAACATGGGGGTTCCGGCCTTCAATATCGCCTCTTCGGTAACCCTCATCATGGCCCAGC
GCCTCGCTCGCAAGCTCTGCGATCACTGCAAGGCGCCGGAAGTTGTGCCCGAAGCTGAATTGCTGGAGCTGGGCTTCACC
CAGCAGCAGTTGGCCGCCGGCTTGCGCCTGTTCAAGCCAGTAGGCTGCAAAGAGTGCTCGGGGGGCTACAAGGGTCGGGT
CGGTATCTACGAAATCATGCTAATGTCTGAAAACATTGCAAAATTGATCATGCAAGGGGCCAACTCCCTGCAAATTGCAG
CGATTGCTCAAAAAGAGGGGATGCGAACCCTGCGTGTCTCGGGCCTGGAGAAGGCCCGCCTGGGGGTGACCAGCCTGGCA
GAGATCAACCGGGTGACCACCAACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.614

100

0.558
  pilB Acinetobacter baumannii D1279779

55.556

99.824

0.555
  pilB Legionella pneumophila strain ERS1305867

54.77

99.648

0.546
  pilB Vibrio cholerae strain A1552

54.513

99.472

0.542
  pilB Vibrio campbellii strain DS40M4

52.12

99.648

0.519
  pilB Vibrio parahaemolyticus RIMD 2210633

51.596

99.296

0.512
  pilF Neisseria gonorrhoeae MS11

46.454

99.296

0.461
  pilB/pilB1 Synechocystis sp. PCC 6803

38.879

97.359

0.379
  pilF Thermus thermophilus HB27

40.341

92.958

0.375