Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   ONZ66_RS02020 Genome accession   NZ_CP110647
Coordinates   406119..406994 (-) Length   291 a.a.
NCBI ID   WP_125729778.1    Uniprot ID   -
Organism   Aeromonas salmonicida strain ZAS     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 401119..411994
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ONZ66_RS01995 (ONZ66_01995) secA 401149..403869 (+) 2721 WP_271774094.1 preprotein translocase subunit SecA -
  ONZ66_RS02000 (ONZ66_02000) mutT 404036..404443 (+) 408 WP_099994491.1 8-oxo-dGTP diphosphatase MutT -
  ONZ66_RS02005 (ONZ66_02005) yacG 404521..404715 (-) 195 WP_059113458.1 DNA gyrase inhibitor YacG -
  ONZ66_RS02010 (ONZ66_02010) zapD 404725..405447 (-) 723 WP_021140336.1 cell division protein ZapD -
  ONZ66_RS02015 (ONZ66_02015) coaE 405485..406099 (-) 615 WP_271774095.1 dephospho-CoA kinase -
  ONZ66_RS02020 (ONZ66_02020) pilD 406119..406994 (-) 876 WP_125729778.1 prepilin peptidase Machinery gene
  ONZ66_RS02025 (ONZ66_02025) pilC 407058..408299 (-) 1242 WP_059113460.1 type II secretion system F family protein Machinery gene
  ONZ66_RS02030 (ONZ66_02030) pilB 408428..410134 (-) 1707 WP_271774096.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  ONZ66_RS02035 (ONZ66_02035) - 410137..410574 (-) 438 WP_271774097.1 pilin -
  ONZ66_RS02040 (ONZ66_02040) nadC 410910..411767 (-) 858 WP_271774098.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 291 a.a.        Molecular weight: 32437.73 Da        Isoelectric Point: 7.0169

>NTDB_id=755641 ONZ66_RS02020 WP_125729778.1 406119..406994(-) (pilD) [Aeromonas salmonicida strain ZAS]
MTLLLELAHGLPWLYFSLVFLFSLMIGSFLNVVIHRLPIMLEREWQAEYRSYFSSDTPQPEADERYNLMVPRSCCPHCNH
PITALENIPLLSWLWLKGRCRGCQAAISARYPLVELLTALLSVVVAMTLTPGWGTLAALLLTWVLVALTFIDLDKMLLPD
QLTLPLLWGGLLFNLLGGYVPLGDAVIGAMAGYLVLWSLYWAFKLLTGKEGMGYGDFKLLAALGAWLGWQALPIVLLLSS
LVGAIFGIGLILLRNHHQSKPIPFGPYLAIAGWIALLWGDSITRWYLSTIL

Nucleotide


Download         Length: 876 bp        

>NTDB_id=755641 ONZ66_RS02020 WP_125729778.1 406119..406994(-) (pilD) [Aeromonas salmonicida strain ZAS]
ATGACACTTCTTCTCGAGCTGGCACACGGCTTGCCATGGCTCTACTTCTCGCTGGTATTTCTCTTCTCCCTGATGATCGG
CAGCTTTCTCAACGTGGTGATCCACCGCCTGCCCATCATGCTGGAACGGGAGTGGCAGGCCGAATATCGCAGCTATTTCA
GTTCAGACACGCCGCAGCCGGAAGCTGACGAGCGCTACAATCTGATGGTGCCGCGCTCCTGCTGCCCTCACTGCAATCAC
CCCATAACGGCCCTGGAAAACATTCCCCTGCTGAGCTGGCTCTGGCTCAAAGGTCGCTGCCGGGGCTGCCAGGCCGCCAT
TTCGGCTCGCTATCCGCTGGTGGAGCTGCTGACCGCTCTGCTCTCTGTGGTGGTTGCCATGACGCTCACCCCTGGCTGGG
GCACGCTGGCTGCCCTCCTGCTGACCTGGGTGCTGGTGGCGCTCACCTTTATCGATCTGGACAAGATGCTGCTGCCGGAC
CAGCTCACCTTGCCGCTACTGTGGGGCGGGCTGCTGTTCAACCTGCTTGGCGGATACGTGCCTCTCGGCGATGCTGTCAT
CGGCGCCATGGCAGGCTATCTGGTGCTGTGGAGCCTCTACTGGGCCTTCAAGCTGCTGACCGGCAAGGAGGGCATGGGCT
ATGGGGACTTCAAACTGCTGGCAGCGCTGGGAGCCTGGCTTGGCTGGCAGGCACTGCCCATAGTACTGCTGCTCTCTTCT
CTGGTCGGCGCCATCTTCGGCATTGGCCTCATTTTGCTGCGCAACCATCACCAGAGCAAACCCATCCCCTTTGGCCCCTA
TCTCGCCATCGCAGGCTGGATCGCACTGCTCTGGGGCGATAGCATCACCCGCTGGTATCTCTCCACTATCCTCTGA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(140-248)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-128)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

61.111

98.969

0.605
  pilD Vibrio campbellii strain DS40M4

55.556

98.969

0.55
  pilD Acinetobacter baumannii D1279779

55.019

92.44

0.509
  pilD Acinetobacter nosocomialis M2

55.019

92.44

0.509
  pilD Neisseria gonorrhoeae MS11

50.373

92.096

0.464