Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ONZ54_RS02085 Genome accession   NZ_CP110645
Coordinates   418072..419778 (-) Length   568 a.a.
NCBI ID   WP_058394588.1    Uniprot ID   -
Organism   Aeromonas salmonicida strain AS3     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 413072..424778
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ONZ54_RS02055 (ONZ54_02055) mutT 413681..414088 (+) 408 WP_099994491.1 8-oxo-dGTP diphosphatase MutT -
  ONZ54_RS02060 (ONZ54_02060) yacG 414166..414360 (-) 195 WP_005314167.1 DNA gyrase inhibitor YacG -
  ONZ54_RS02065 (ONZ54_02065) zapD 414370..415092 (-) 723 WP_005314166.1 cell division protein ZapD -
  ONZ54_RS02070 (ONZ54_02070) coaE 415130..415744 (-) 615 WP_271787346.1 dephospho-CoA kinase -
  ONZ54_RS02075 (ONZ54_02075) pilD 415763..416638 (-) 876 WP_125729778.1 prepilin peptidase Machinery gene
  ONZ54_RS02080 (ONZ54_02080) pilC 416702..417943 (-) 1242 WP_059113460.1 type II secretion system F family protein Machinery gene
  ONZ54_RS02085 (ONZ54_02085) pilB 418072..419778 (-) 1707 WP_058394588.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  ONZ54_RS02090 (ONZ54_02090) - 419781..420218 (-) 438 WP_271787347.1 pilin -
  ONZ54_RS02095 (ONZ54_02095) nadC 420552..421409 (-) 858 WP_219614709.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ONZ54_RS02100 (ONZ54_02100) - 421414..421883 (-) 470 Protein_392 retropepsin-like aspartic protease family protein -
  ONZ54_RS02105 (ONZ54_02105) ampD 422033..422605 (+) 573 WP_271788237.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ONZ54_RS02110 (ONZ54_02110) pdhR 422882..423646 (+) 765 WP_005314150.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62546.97 Da        Isoelectric Point: 5.4384

>NTDB_id=755614 ONZ54_RS02085 WP_058394588.1 418072..419778(-) (pilB) [Aeromonas salmonicida strain AS3]
MTSSPNSGLALSLAASSLLSESDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTAAIGKLLESEQDALGMEEIDESEI
SELEVSDENSRLDESVNTADDDAPIVKYINKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEIATPPVNLANRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
QQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRVSGLEKARLGVTSLA
EINRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=755614 ONZ54_RS02085 WP_058394588.1 418072..419778(-) (pilB) [Aeromonas salmonicida strain AS3]
ATGACCTCTAGCCCCAATAGCGGCCTGGCGTTAAGCCTGGCCGCCTCATCCCTGCTCAGTGAGTCCGACTCACAGCGTTA
CCTGAGCCAAGCCAAGGCCCAGCGCAAACCCTTCGTGACATTTCTGATTGAAAACGAGATCCTCGACAGCAAGGCGCTGG
CGGATTTTTGCGAGCTGGAATATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACCTGGCCGAGATCCCGCAGAAGTAC
CTCAATCAGAAGCTGATTGAGAAACACCATGTGCTGCCCATCTACACCCAGGGCCACACCCTCTATATCGCCATGTCGGA
CCCGACCAATGTGTCGGCACTGGAGGATTTTGGCTTCAGCTTCGGACTCCATACCGAAGCCCTGCTGGTGGAAGAGAACA
AGCTCACCGCCGCCATCGGCAAGCTGCTGGAAAGCGAGCAGGATGCCCTCGGCATGGAGGAGATCGACGAGTCGGAGATC
TCCGAGCTGGAAGTCTCGGACGAAAACTCCCGCCTCGATGAGAGCGTCAATACCGCCGACGACGATGCCCCCATCGTCAA
ATACATCAATAAAATCATGATGGATGCCATCAAGCGCGGCGCCTCGGATCTGCACTTCGAACCCTATGAGACCAAGTACC
GTATCCGCTTTCGTATAGACGGCATACTGCACGAAATAGCGACGCCGCCAGTCAATCTGGCCAACCGCTTTTCGGCCCGC
CTCAAGGTGATGGCGCGACTCGATATCGCAGAGCGACGGCTGCCCCAGGATGGTCGCATCAAGTTAAAGCTGTCGCGCAA
CAAATCCATGGATATGCGGGTCAACACACTGCCCACCATGTGGGGCGAGAAGATCGTTATCCGTCTGCTGGACTCTTCCG
CAGCGCGACTCAATATCGAACAGCTCGGCTTCGACGACCGGCAGAAGGCACAGTATCTGCGTGCCCTCTCCAAGCCCCAG
GGCATGATATTGGTGACAGGCCCGACCGGCTCTGGCAAGACTGTCTCTCTCTATACCGGCCTCAATATTCTCAACACCAC
AGAGGTCAACATCTCTACCGCCGAAGACCCAGTGGAGATCAACCTGCCCGGCGTCAACCAGGTACAAGTCAATCCCAAGG
CGGGGCTGACCTTCGCCAGCGCCCTGCGCTCCTTTCTTCGCCAGGATCCCGATGTGGTAATGGTGGGGGAAATCCGGGAT
CTGGAAACCGCCGAGATTGCCATCAAAGCCGCCCAGACCGGCCACCTGGTGCTCTCCACCCTGCACACCAACTCGGCTGC
CGAAACCCTGACCCGGATGATGAACATGGGGGTTCCGGCCTTCAACATCGCCTCTTCGGTAACCCTCATCATGGCCCAGC
GCCTCGCTCGCAAGCTCTGCGATCACTGCAAGGCGCCGGAAGTGGTGCCCGAGGCTGAACTGCTGGAGCTGGGCTTCACC
CAGCAGCAGTTGGCCGCCGGCTTGCGCCTGTTCAAACCGGTAGGCTGCAAAGAGTGCTCGGGGGGCTACAAGGGTCGGGT
CGGTATCTACGAAATCATGCTAATGTCTGAAAACATTGCAAAATTGATCATGCAAGGAGCCAACTCCCTGCAAATTGCAG
CGATTGCCCAGAAAGAGGGGATGCGAACCCTGCGTGTCTCGGGCCTGGAGAAGGCCCGCCTGGGGGTGACCAGCCTGGCA
GAGATCAACCGGGTGACCACCAACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.614

100

0.558
  pilB Acinetobacter baumannii D1279779

55.556

99.824

0.555
  pilB Legionella pneumophila strain ERS1305867

54.594

99.648

0.544
  pilB Vibrio cholerae strain A1552

54.513

99.472

0.542
  pilB Vibrio campbellii strain DS40M4

52.12

99.648

0.519
  pilB Vibrio parahaemolyticus RIMD 2210633

51.596

99.296

0.512
  pilF Neisseria gonorrhoeae MS11

46.454

99.296

0.461
  pilB/pilB1 Synechocystis sp. PCC 6803

38.879

97.359

0.379
  pilF Thermus thermophilus HB27

40.341

92.958

0.375