Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ONZ46_RS01000 Genome accession   NZ_CP110415
Coordinates   191740..192249 (+) Length   169 a.a.
NCBI ID   WP_272887741.1    Uniprot ID   -
Organism   Caldimonas thermodepolymerans strain LMG 21645     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 186740..197249
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ONZ46_RS00975 (ONZ46_00975) - 186926..187711 (+) 786 WP_104357386.1 Stp1/IreP family PP2C-type Ser/Thr phosphatase -
  ONZ46_RS00980 (ONZ46_00980) - 187744..188421 (+) 678 WP_104357387.1 FHA domain-containing protein -
  ONZ46_RS00985 (ONZ46_00985) - 188528..190777 (+) 2250 WP_265136400.1 CHASE2 domain-containing protein -
  ONZ46_RS00990 (ONZ46_00990) - 190815..191576 (+) 762 WP_265136401.1 MBL fold metallo-hydrolase -
  ONZ46_RS01000 (ONZ46_01000) pilA 191740..192249 (+) 510 WP_272887741.1 pilin Machinery gene
  ONZ46_RS01005 (ONZ46_01005) - 192349..194262 (+) 1914 WP_265136402.1 sulfatase-like hydrolase/transferase -
  ONZ46_RS01010 (ONZ46_01010) - 194526..196241 (+) 1716 WP_265136403.1 PglL family O-oligosaccharyltransferase -
  ONZ46_RS01015 (ONZ46_01015) moaC 196249..196755 (-) 507 WP_265136404.1 cyclic pyranopterin monophosphate synthase MoaC -

Sequence

Protein


Download         Length: 169 a.a.        Molecular weight: 16602.87 Da        Isoelectric Point: 8.1096

>NTDB_id=754878 ONZ46_RS01000 WP_272887741.1 191740..192249(+) (pilA) [Caldimonas thermodepolymerans strain LMG 21645]
MKQLRPIQRVQQGFTLIELMIVVAIIGILAAIALPAYQDYTIRARVSEALSIASGAKATVAENIANNGGLLPTDACVGVN
TNLGTGGKNVASLTCTAASGAIAVTTTQAAGNLTLTLTPTAPGSDGGSVTWACTVNAQEKVKYVPAECRTVGGGSGGGSG
GGSGGGSNP

Nucleotide


Download         Length: 510 bp        

>NTDB_id=754878 ONZ46_RS01000 WP_272887741.1 191740..192249(+) (pilA) [Caldimonas thermodepolymerans strain LMG 21645]
ATGAAGCAACTTCGTCCCATCCAGCGCGTGCAACAAGGTTTCACGCTGATCGAACTGATGATCGTGGTGGCGATCATCGG
TATCCTGGCCGCCATCGCGCTGCCCGCCTATCAGGACTACACCATCCGTGCCCGCGTGTCCGAAGCGCTGTCGATCGCGT
CCGGCGCCAAGGCCACAGTGGCCGAAAACATCGCCAACAACGGTGGCTTGCTGCCCACCGATGCCTGCGTCGGCGTGAAC
ACCAACCTGGGCACCGGCGGCAAGAACGTCGCGAGCCTGACCTGCACGGCCGCTTCGGGCGCGATTGCGGTTACGACCAC
GCAGGCTGCGGGCAACCTGACTCTGACCCTGACCCCGACGGCTCCCGGTTCTGACGGTGGTTCGGTGACCTGGGCGTGCA
CGGTGAATGCGCAAGAGAAGGTCAAGTACGTGCCGGCTGAGTGCCGCACCGTGGGTGGTGGCTCCGGTGGTGGCTCCGGT
GGTGGCTCCGGTGGTGGTTCCAACCCCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(43-149)

Prokaryotic N-terminal methylation motif (N_methyl)

(9-34)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

49.432

100

0.515
  comP Acinetobacter baylyi ADP1

56

88.757

0.497
  pilA2 Legionella pneumophila str. Paris

53.957

82.249

0.444
  pilA2 Legionella pneumophila strain ERS1305867

53.237

82.249

0.438
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

35.979

100

0.402
  pilA/pilAI Pseudomonas stutzeri DSM 10701

42.384

89.349

0.379
  pilE Neisseria gonorrhoeae strain FA1090

39.623

94.083

0.373
  pilA/pilA1 Eikenella corrodens VA1

40.132

89.941

0.361