Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PALA52_RS23250 Genome accession   NZ_CP110347
Coordinates   5041192..5042892 (+) Length   566 a.a.
NCBI ID   WP_024914668.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain PALA52     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5036192..5047892
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PALA52_RS23225 (PALA52_04612) ampD 5036202..5036768 (-) 567 WP_024914671.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  PALA52_RS23230 (PALA52_04613) - 5036919..5039174 (-) 2256 WP_263957286.1 DUF1631 domain-containing protein -
  PALA52_RS23235 (PALA52_04614) nadC 5039460..5040308 (+) 849 WP_058141272.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PALA52_RS23245 pilA 5040510..5040959 (-) 450 WP_003112842.1 type 4a pilus biogenesis protein PilA Machinery gene
  PALA52_RS23250 (PALA52_04616) pilB 5041192..5042892 (+) 1701 WP_024914668.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PALA52_RS23255 (PALA52_04617) pilC 5042896..5044116 (+) 1221 WP_024914667.1 type 4a pilus biogenesis protein PilC Machinery gene
  PALA52_RS23260 (PALA52_04618) pilD 5044120..5044992 (+) 873 WP_003125204.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  PALA52_RS23265 (PALA52_04619) coaE 5044989..5045600 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  PALA52_RS23270 (PALA52_04620) yacG 5045597..5045797 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  PALA52_RS23275 (PALA52_04621) - 5045835..5046044 (-) 210 WP_003094660.1 hypothetical protein -
  PALA52_RS23280 (PALA52_04622) - 5046150..5046839 (-) 690 WP_058141274.1 energy-coupling factor ABC transporter permease -
  PALA52_RS23285 (PALA52_04623) - 5046836..5047306 (-) 471 WP_024914665.1 hypothetical protein -
  PALA52_RS23290 (PALA52_04624) - 5047303..5047728 (-) 426 WP_024914664.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62386.27 Da        Isoelectric Point: 5.5590

>NTDB_id=754273 PALA52_RS23250 WP_024914668.1 5041192..5042892(+) (pilB) [Pseudomonas aeruginosa strain PALA52]
MNDSIQLSGLSRQLVQANLLDEKTAVQAQAQAQRNKLSLVTHLVQNKLVSGLALAELSAEQFGIAYCDLNSLDKESFPRD
AISEKLVRQHRVIPLWRRGNKLFVGISDPANHQAINDVQFSTGLTTEAILVEDDKLGLAIDKLFESATDGLAGLDDVDLE
GLDIGSTEKSTQEDTSAEADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKIYRVRFRTDGMLHEVAKPPIQLASRISARL
KVMAGLDISERRKPQDGRIKMRVSKTKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHDVPKETLLHEGFPE
ELIGTFKLYSPVGCDHCKNGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQARKEGFNDLRTSGLLKAMQGITSLEEV
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=754273 PALA52_RS23250 WP_024914668.1 5041192..5042892(+) (pilB) [Pseudomonas aeruginosa strain PALA52]
ATGAACGACAGCATCCAACTGAGCGGCCTGTCCCGACAGCTCGTCCAAGCCAATCTACTCGACGAAAAGACCGCCGTGCA
GGCCCAGGCGCAGGCGCAGCGCAACAAGCTGTCGCTGGTGACGCACCTGGTGCAGAACAAGCTGGTAAGCGGCCTGGCCC
TGGCCGAATTATCCGCCGAGCAGTTCGGTATCGCCTACTGCGACCTGAATAGCCTGGACAAGGAAAGCTTTCCGCGGGAC
GCCATCAGCGAGAAGCTGGTCCGCCAGCACCGGGTCATCCCTTTGTGGCGGCGCGGCAACAAGCTCTTCGTGGGCATCTC
CGACCCCGCCAACCACCAGGCCATCAATGATGTCCAGTTCAGCACCGGCCTGACGACCGAAGCGATTCTGGTCGAAGACG
ACAAGCTTGGCCTGGCCATCGACAAACTGTTCGAAAGCGCTACCGATGGTCTCGCAGGCCTCGACGATGTCGACCTCGAG
GGACTGGATATCGGTAGCACGGAAAAGTCCACCCAGGAGGATACCAGCGCAGAAGCGGACGACGCTCCTGTAGTCCGTTT
CGTCAACAAGATGCTGCTGGATGCCATCAAGGGCGGCTCTTCCGACCTGCACTTCGAGCCCTACGAGAAGATCTACCGGG
TGCGCTTCCGTACCGACGGCATGCTCCACGAAGTGGCCAAGCCGCCGATCCAGCTCGCCAGCCGCATCTCCGCGCGACTC
AAGGTGATGGCCGGCCTGGATATCTCCGAACGACGCAAGCCGCAGGACGGGCGGATCAAGATGCGCGTGTCGAAGACCAA
GTCCATCGACTTCCGGGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGATCGTGATGCGGATCCTCGACTCCTCCAGCG
CGCAGATGGGCATCGACGCCCTCGGCTACGAAGAGGACCAGAAGGAACTCTACCTGGCCGCGCTCAAGCAGCCGCAGGGC
ATGATCCTGGTCACCGGCCCCACCGGTTCGGGCAAGACGGTCTCGCTGTACACCGGCCTGAACATCCTCAATACCACCGA
CATCAACATCTCCACCGCCGAAGACCCGGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAACCCGCGCCAGG
GCATGGACTTCTCCCAGGCACTGCGCGCCTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGACCTG
GAGACCGCCGAGATCGCCATCAAGGCGGCGCAGACCGGGCATATGGTGATGTCCACCCTGCACACCAACAGCGCCGCAGA
GACCCTGACCCGGCTGCTGAACATGGGCGTGCCGGCGTTCAACCTGGCGACCTCGGTGAACCTGATCATCGCCCAGCGCC
TTGCGCGAAAACTCTGTTCGCACTGCAAGAAAGAGCACGACGTACCGAAGGAAACCCTGCTTCACGAAGGTTTCCCGGAA
GAGTTGATCGGCACCTTCAAGCTGTATTCGCCAGTGGGCTGCGACCATTGCAAGAACGGTTACAAGGGCCGTGTCGGTAT
TTATGAAGTAGTTAAAAACACCCCGGCCCTGCAGCGGATTATCATGGAAGAAGGCAACTCTATCGAGATCGCCGAGCAAG
CCCGCAAAGAAGGCTTCAACGATCTGCGCACCTCAGGCCTGCTGAAAGCCATGCAGGGAATCACCAGCCTGGAGGAAGTC
AACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-147)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.867

100

0.585
  pilB Acinetobacter baylyi ADP1

57.194

99.47

0.569
  pilB Legionella pneumophila strain ERS1305867

55.124

100

0.551
  pilB Vibrio cholerae strain A1552

50.526

100

0.509
  pilB Vibrio parahaemolyticus RIMD 2210633

50.799

99.47

0.505
  pilF Neisseria gonorrhoeae MS11

50.534

99.293

0.502
  pilB Vibrio campbellii strain DS40M4

49.645

99.647

0.495
  pilF Thermus thermophilus HB27

39.394

99.117

0.39
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.607

93.11

0.378
  pilB/pilB1 Synechocystis sp. PCC 6803

38.764

94.346

0.366