Detailed information NTG0754143

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: LJ360_RS16570
• Genome accession: NZ_CP110342
• Coordinates: 3578689..3579810 (-)
• Length: 373 a.a.
• NCBI ID: WP_091852631.1
• Uniprot ID: A0A1I0F985
• Organism: Marinobacter sp. AN1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3573689..3584810

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LJ360_RS16550 (LJ360_16550)	-	3574424..3574870 (+)	447	WP_264994481.1	fibronectin type III domain-containing protein	-
LJ360_RS16555 (LJ360_16555)	-	3574904..3575248 (-)	345	WP_091852625.1	DUF2782 domain-containing protein	-
LJ360_RS16560 (LJ360_16560)	polA	3575347..3578067 (+)	2721	WP_091852628.1	DNA polymerase I	-
LJ360_RS16565 (LJ360_16565)	-	3578271..3578630 (+)	360	WP_091852814.1	hypothetical protein	-
LJ360_RS16570 (LJ360_16570)	pilU	3578689..3579810 (-)	1122	WP_091852631.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
LJ360_RS16575 (LJ360_16575)	pilT	3579883..3580917 (-)	1035	WP_091852634.1	type IV pilus twitching motility protein PilT	Machinery gene
LJ360_RS16580 (LJ360_16580)	-	3581028..3581732 (+)	705	WP_091852636.1	YggS family pyridoxal phosphate-dependent enzyme	-
LJ360_RS16585 (LJ360_16585)	proC	3581853..3582689 (+)	837	WP_091852638.1	pyrroline-5-carboxylate reductase	-
LJ360_RS16590 (LJ360_16590)	-	3582733..3583302 (+)	570	WP_091852641.1	YggT family protein	-

Sequence

Protein

• Length: 373 a.a.
• Molecular weight: 41374.45 Da
• Isoelectric Point: 6.6611

>NTDB_id=754143 LJ360_RS16570 WP_091852631.1 3578689..3579810(-) (pilU) [Marinobacter sp. AN1]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKVLPVTKNALTPEQTRELVYGAMNSRQQAEFEESHECNFAISARGIGR
FRVSAFFQRNLCGMVLRRIETKIPQVDDLGLPEIIKDLAMTKRGLIIFVGATGTGKSTSLAAMLGHRNRNSRGHIISIED
PIEFVHQHQGCIVTQREVGIDTESFETALKNTLRQAPDVILIGEIRTRETMQYAVQFAETGHLCLATLHANNANQALDRI
IQFFNPDQHNQTWMDLSLNLKAIVAQQLIPTPDGKGRRAVIEVLINTPLVADMIRKGEVHKLKELMTKSTESGMQTFDQA
LYSLYSEGAITYEDALAHADSANDLRLMIKLGADAKGSDQLSTTVDKLSIQND

Nucleotide

• Length: 1122 bp

>NTDB_id=754143 LJ360_RS16570 WP_091852631.1 3578689..3579810(-) (pilU) [Marinobacter sp. AN1]
ATGGAATTTGAAAAACTGCTGCGACTGATGGTGGAAAAGGGCGGCTCTGACCTGTTTATTACCGCCGGGGTTCCACCGAG
CATGAAAGTCAACGGCAAGGTACTGCCCGTCACCAAGAACGCCCTGACCCCGGAGCAGACCAGGGAGCTTGTCTATGGCG
CCATGAACTCCAGGCAACAGGCGGAGTTCGAGGAAAGCCACGAGTGCAACTTTGCCATCAGCGCCCGGGGCATCGGTCGC
TTCCGGGTCAGCGCCTTCTTCCAGCGCAACCTGTGTGGCATGGTGCTGCGGCGCATCGAGACCAAGATTCCACAGGTGGA
TGACCTGGGGCTGCCGGAGATCATCAAGGACCTGGCGATGACCAAACGGGGTCTGATCATCTTCGTGGGGGCTACCGGTA
CCGGTAAGTCCACCTCGCTGGCGGCCATGCTGGGGCACCGCAACCGCAACAGCCGCGGCCACATCATCTCCATCGAGGAC
CCCATTGAATTCGTCCACCAGCACCAGGGCTGCATCGTTACCCAGCGGGAAGTGGGCATCGATACCGAGAGTTTCGAAAC
GGCCCTGAAAAACACCCTGCGGCAGGCTCCGGATGTGATCCTGATCGGTGAGATCCGGACCCGGGAGACTATGCAGTACG
CTGTGCAGTTTGCAGAAACCGGTCACCTCTGCCTGGCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGGATT
ATCCAGTTCTTCAACCCGGACCAGCACAACCAGACCTGGATGGACCTGTCCCTGAACCTGAAGGCCATCGTCGCCCAGCA
GCTGATTCCCACACCGGACGGCAAGGGGCGCCGGGCGGTTATCGAGGTGTTGATCAACACCCCGCTGGTGGCGGATATGA
TCCGCAAGGGCGAGGTGCACAAGCTCAAGGAGTTGATGACCAAGTCTACCGAGTCAGGTATGCAGACCTTCGACCAGGCC
CTGTACAGCCTTTACTCGGAAGGTGCCATCACCTATGAGGATGCCCTGGCCCATGCCGACTCTGCCAACGACTTGCGCCT
GATGATCAAGCTGGGCGCGGATGCCAAGGGCTCCGACCAGCTATCGACCACAGTCGACAAGTTGTCTATCCAGAACGACT
GA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (114-270)

3D structure

• AlphaFold DB: A0A1I0F985

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	78.706	99.464	0.783
pilU	Acinetobacter baylyi ADP1	66.292	95.442	0.633
pilU	Vibrio cholerae strain A1552	56.486	99.196	0.56
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.363	90.885	0.394
pilT	Acinetobacter nosocomialis M2	40.597	89.812	0.365
pilT	Acinetobacter baumannii D1279779	40.299	89.812	0.362
pilT	Acinetobacter baumannii strain A118	40.299	89.812	0.362