Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LJ360_RS14295 Genome accession   NZ_CP110342
Coordinates   3121596..3123314 (+) Length   572 a.a.
NCBI ID   WP_091852308.1    Uniprot ID   -
Organism   Marinobacter sp. AN1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3116596..3128314
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LJ360_RS14280 (LJ360_14280) - 3117903..3118994 (-) 1092 WP_264994393.1 IS630 family transposase -
  LJ360_RS14285 (LJ360_14285) - 3119199..3120611 (-) 1413 WP_264994394.1 IS1182 family transposase -
  LJ360_RS14290 (LJ360_14290) - 3120708..3121214 (-) 507 WP_320108770.1 pilin -
  LJ360_RS14295 (LJ360_14295) pilB 3121596..3123314 (+) 1719 WP_091852308.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LJ360_RS14300 (LJ360_14300) pilC 3123317..3124543 (+) 1227 WP_264994395.1 type II secretion system F family protein Machinery gene
  LJ360_RS14305 (LJ360_14305) pilD 3124710..3125588 (+) 879 WP_264994396.1 prepilin peptidase Machinery gene
  LJ360_RS14310 (LJ360_14310) - 3125621..3126601 (-) 981 WP_264994397.1 D-2-hydroxyacid dehydrogenase -
  LJ360_RS14315 (LJ360_14315) - 3126802..3128025 (+) 1224 WP_091852320.1 pyridoxal phosphate-dependent aminotransferase -
  LJ360_RS14320 (LJ360_14320) - 3128097..3128282 (+) 186 WP_091852323.1 CPXCG motif-containing cysteine-rich protein -

Sequence

Protein


Download         Length: 572 a.a.        Molecular weight: 62993.10 Da        Isoelectric Point: 4.7473

>NTDB_id=754138 LJ360_RS14295 WP_091852308.1 3121596..3123314(+) (pilB) [Marinobacter sp. AN1]
MATDKSNVTLTGLARRFVEDGLLEEAVAKDAYLQAANNRIPLITYLVQNDLADSQKLAFAAAMEFGVSVLDLNSFLPEML
PEKLVDEKLVRKHNALPLYKRGNRLFIAVSDPTNIQALDEIKFNTGLSTDAILVDDAALRIAIDKYLESAEDAMGDLDDA
DLEGVETEGGEQDDDDAVVATTDVDDAPIVKYVNKILLDAIRGGASDIHFEPYEKVYRVRYRTDGMLKEISRPSIKLAPK
ISARVKIMAQLDISERRVPQDGRIKMKLSKTKAIDFRVNTLPTLWGEKIVLRILDPSQAKMGIDALGYEEDQKELYMNAL
KQPQGMILVTGPTGSGKTVSLYTGLNILNQPEINISTAEDPAEINLEGINQVNVNNKVGLGFAEALRAFLRQDPDVIMVG
EIRDLETANIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPSFNIATSVSLIIAQRLGRRLCNSCKQPLDVPKETLLK
EGFTEEQIETGFTLYTPKGCDKCNGGYKGRVGIYEVVKVTESLANMIMEEASSIQIARQAQQEGFRTLRQSALRKVIEGV
TSLEEANRVTKD

Nucleotide


Download         Length: 1719 bp        

>NTDB_id=754138 LJ360_RS14295 WP_091852308.1 3121596..3123314(+) (pilB) [Marinobacter sp. AN1]
ATGGCAACCGATAAAAGCAATGTAACTCTTACGGGCCTGGCCCGGCGATTTGTAGAGGACGGCCTGTTAGAGGAGGCTGT
CGCCAAGGATGCCTACCTGCAGGCAGCCAATAATCGCATTCCCCTTATCACCTACCTGGTCCAGAACGACCTGGCGGACA
GCCAGAAGCTGGCGTTTGCAGCGGCCATGGAGTTCGGCGTCAGTGTTCTGGACCTGAACAGCTTTCTACCGGAGATGCTG
CCGGAAAAGCTGGTGGACGAAAAACTCGTTCGCAAGCACAACGCCCTGCCTCTGTATAAACGAGGCAATCGGCTGTTTAT
TGCCGTATCTGACCCCACCAATATCCAGGCCCTCGATGAGATCAAGTTCAACACTGGCCTGAGTACCGACGCGATCCTGG
TGGATGATGCCGCGCTGCGGATCGCCATCGACAAGTATCTGGAATCTGCTGAAGACGCCATGGGCGATCTTGACGATGCG
GATCTGGAGGGCGTGGAAACAGAGGGTGGTGAGCAGGACGACGATGACGCTGTCGTTGCAACCACGGATGTGGACGACGC
TCCCATCGTAAAGTACGTCAACAAGATCCTGCTGGATGCGATTCGGGGCGGAGCTTCGGATATCCACTTTGAGCCCTATG
AAAAGGTGTACCGGGTGCGCTACCGCACCGACGGCATGCTGAAGGAAATTTCCCGCCCCTCCATCAAACTGGCCCCCAAG
ATCTCCGCCCGGGTAAAGATCATGGCCCAGCTGGATATTTCCGAGCGCCGGGTACCCCAGGATGGCCGCATCAAGATGAA
GTTATCCAAGACCAAGGCCATCGACTTCCGGGTCAACACCCTGCCTACCCTGTGGGGTGAAAAGATCGTGTTGCGGATCC
TGGATCCCAGCCAGGCGAAGATGGGCATCGATGCGTTGGGTTATGAGGAAGACCAGAAAGAACTCTACATGAATGCCCTC
AAGCAGCCTCAGGGCATGATCCTGGTGACCGGTCCTACCGGCTCTGGTAAGACGGTATCCCTCTACACCGGCCTGAATAT
TCTCAACCAGCCCGAGATCAACATCTCGACGGCGGAAGACCCGGCGGAAATCAACCTGGAGGGCATCAACCAGGTCAATG
TGAACAACAAGGTGGGCCTGGGCTTTGCCGAAGCCCTGCGGGCGTTCCTGCGGCAAGACCCGGATGTGATCATGGTGGGT
GAGATCCGTGACCTGGAAACTGCCAATATTGCTATCAAGGCGGCCCAGACCGGCCACCTGGTGCTGTCCACCCTGCACAC
CAACAGCGCGGCAGAGACCCTGACCCGGATGATGAACATGGGTGTACCGTCGTTCAACATCGCCACATCGGTCAGCCTGA
TCATTGCCCAGCGCCTGGGCCGGCGCCTGTGTAACAGCTGCAAACAGCCGCTGGATGTGCCGAAAGAAACGCTTTTAAAG
GAAGGGTTCACAGAAGAACAAATTGAAACTGGCTTTACGTTGTACACACCCAAGGGCTGTGATAAATGCAATGGAGGCTA
TAAGGGCCGCGTGGGTATCTACGAGGTGGTGAAAGTTACCGAGTCACTGGCGAACATGATTATGGAAGAAGCCAGCTCCA
TCCAGATAGCCCGCCAGGCCCAGCAAGAGGGTTTCCGTACGCTGCGTCAGTCCGCCCTTCGCAAGGTCATTGAAGGGGTG
ACCAGCCTTGAGGAAGCCAACCGCGTAACGAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-151)

Type II/IV secretion system protein (T2SSE)

(195-462)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.597

100

0.57
  pilB Acinetobacter baylyi ADP1

56.383

98.601

0.556
  pilB Legionella pneumophila strain ERS1305867

53.993

100

0.544
  pilB Vibrio cholerae strain A1552

53.918

93.706

0.505
  pilB Vibrio campbellii strain DS40M4

50.973

98.776

0.503
  pilB Vibrio parahaemolyticus RIMD 2210633

51.171

97.028

0.497
  pilF Neisseria gonorrhoeae MS11

47.619

99.126

0.472
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.72

92.308

0.376
  pilB/pilB1 Synechocystis sp. PCC 6803

41.346

90.909

0.376
  pilF Thermus thermophilus HB27

39.089

92.133

0.36