Detailed information NTG0753677

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: OM992_RS13430
• Genome accession: NZ_CP110268
• Coordinates: 2618221..2618835 (-)
• Length: 204 a.a.
• NCBI ID: WP_264818854.1
• Uniprot ID: -
• Organism: Bacillus siamensis strain YB-1631
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2613221..2623835

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OM992_RS13400 (OM992_13400)	rpsT	2613521..2613787 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
OM992_RS13405 (OM992_13405)	holA	2613811..2614846 (-)	1036	Protein_2590	DNA polymerase III subunit delta	-
OM992_RS13410 (OM992_13410)	-	2614886..2615040 (-)	155	Protein_2591	hypothetical protein	-
OM992_RS13415 (OM992_13415)	-	2615081..2615215 (+)	135	WP_003152870.1	YqzM family protein	-
OM992_RS13420 (OM992_13420)	comEC	2615234..2617585 (-)	2352	WP_264818853.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
OM992_RS13425 (OM992_13425)	-	2617586..2618155 (-)	570	WP_047475594.1	ComE operon protein 2	-
OM992_RS13430 (OM992_13430)	comEA	2618221..2618835 (-)	615	WP_264818854.1	helix-hairpin-helix domain-containing protein	Machinery gene
OM992_RS13435 (OM992_13435)	comER	2618893..2619714 (+)	822	WP_264818855.1	late competence protein ComER	-
OM992_RS13440 (OM992_13440)	-	2619783..2620520 (-)	738	WP_064777738.1	class I SAM-dependent methyltransferase	-
OM992_RS13445 (OM992_13445)	rsfS	2620517..2620873 (-)	357	WP_016938891.1	ribosome silencing factor	-
OM992_RS13450 (OM992_13450)	yqeK	2620890..2621450 (-)	561	WP_045926758.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
OM992_RS13455 (OM992_13455)	-	2621440..2622009 (-)	570	WP_016938889.1	nicotinate-nucleotide adenylyltransferase	-
OM992_RS13460 (OM992_13460)	yhbY	2622020..2622310 (-)	291	WP_064777737.1	ribosome assembly RNA-binding protein YhbY	-
OM992_RS13465 (OM992_13465)	aroE	2622304..2623146 (-)	843	WP_016938888.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22345.42 Da
• Isoelectric Point: 5.0246

>NTDB_id=753677 OM992_RS13430 WP_264818854.1 2618221..2618835(-) (comEA) [Bacillus siamensis strain YB-1631]
MTKAMMTEQMRRNLKILGVCLAAGAIFICFWLFRPEKEEPVKQQSVLGQTASASGTDKTDTADDKIMIDMKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKEKADELNVNLAEQLQDGTVVYIPSKGEEENRPKTAAGEKEDAHVNINTATLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=753677 OM992_RS13430 WP_264818854.1 2618221..2618835(-) (comEA) [Bacillus siamensis strain YB-1631]
ATGACGAAAGCAATGATGACGGAGCAGATGCGGCGGAACCTGAAAATTCTGGGTGTTTGTCTTGCGGCAGGAGCGATTTT
TATCTGCTTTTGGCTGTTTCGTCCTGAAAAAGAAGAACCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGGCACGGACAAAACAGACACCGCAGACGATAAGATTATGATTGATATGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAGGCGGGAGACAGAGTGACACAAGCCATTGAGAAAGCCGGCGGCCTGAAGGAAAAGGCGGATGAACTGAATGT
TAATTTAGCAGAGCAGCTGCAAGACGGGACGGTCGTTTATATTCCGAGTAAAGGCGAAGAAGAGAATCGCCCGAAAACTG
CAGCAGGTGAAAAGGAAGATGCCCATGTCAATATCAATACGGCAACTCTGGACGAACTTCAGGCGATTTCCGGTGTGGGA
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGGGAAGAAAACGGACGTTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGTTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (68-122)

• Helix-hairpin-helix motif (HHH_3) (142-202)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	57.5	98.039	0.564
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.715	100	0.373
comEA	Lactococcus lactis subsp. cremoris KW2	36.715	100	0.373
comEA/celA/cilE	Streptococcus pneumoniae D39	36.232	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae R6	36.232	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	36.232	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	36.232	100	0.368
comEA/celA/cilE	Streptococcus mitis NCTC 12261	36.232	100	0.368
comEA	Latilactobacillus sakei subsp. sakei 23K	31.76	100	0.363