Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   OKW95_RS17640 Genome accession   NZ_CP110254
Coordinates   3747988..3749700 (+) Length   570 a.a.
NCBI ID   WP_192835760.1    Uniprot ID   -
Organism   Acinetobacter oleivorans strain S4     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3742988..3754700
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OKW95_RS17605 (OKW95_17610) nusA 3743875..3745359 (-) 1485 WP_003654584.1 transcription termination factor NusA -
  OKW95_RS17610 (OKW95_17615) rimP 3745396..3745923 (-) 528 WP_004795010.1 ribosome maturation factor RimP -
  OKW95_RS17630 (OKW95_17635) secG 3746560..3746889 (-) 330 WP_004795012.1 preprotein translocase subunit SecG -
  OKW95_RS17635 (OKW95_17640) tpiA 3746902..3747696 (-) 795 WP_013199077.1 triose-phosphate isomerase -
  OKW95_RS17640 (OKW95_17645) pilB 3747988..3749700 (+) 1713 WP_192835760.1 type IV-A pilus assembly ATPase PilB Machinery gene
  OKW95_RS17645 (OKW95_17650) pilC 3749728..3750954 (+) 1227 WP_199944927.1 type II secretion system F family protein Machinery gene
  OKW95_RS17650 (OKW95_17655) pilD 3750954..3751814 (+) 861 WP_013199081.1 prepilin peptidase Machinery gene
  OKW95_RS17655 (OKW95_17660) coaE 3751816..3752412 (+) 597 WP_013199082.1 dephospho-CoA kinase -
  OKW95_RS17660 (OKW95_17665) - 3752409..3753323 (-) 915 WP_013199083.1 DMT family transporter -
  OKW95_RS17665 (OKW95_17670) rlmB 3753359..3754108 (-) 750 WP_004795029.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  OKW95_RS17670 (OKW95_17675) - 3754211..3754537 (-) 327 WP_004795031.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63493.84 Da        Isoelectric Point: 4.9650

>NTDB_id=753395 OKW95_RS17640 WP_192835760.1 3747988..3749700(+) (pilB) [Acinetobacter oleivorans strain S4]
MSVIHTSPKFSGFFRRLVEEKHVSAATMQAALDAAKRAKQDTVAYLIEEVNLSPSLLAETISIEFAEPYFDLDVYDTSQI
PKDLVDQKLILKHRILPLVQRGQILYVATSNPSNIDAIDAIRFNSKLMVEPVIVEHHKLEKILVQQFTEESNFEFSDEEF
DLDVDLDTSTAPEEDEDTPQGDEAPIVKYVNKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRQIANPPLQLANRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKSKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEEQKALFMEALDKP
QGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNPKVGLTFSAALKSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKVPTDIPKQSLLEMGF
TEQDLTHPDFQVFQPVGCPECREGYKGRVGIYEVMKVTPEISKIIMEDGNALEIAAASEKLGFNNLRRSGLKKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=753395 OKW95_RS17640 WP_192835760.1 3747988..3749700(+) (pilB) [Acinetobacter oleivorans strain S4]
ATGTCAGTCATACATACATCTCCAAAATTTTCGGGGTTTTTTCGACGATTAGTCGAAGAAAAACATGTGTCGGCAGCGAC
CATGCAAGCAGCACTAGATGCAGCAAAAAGAGCTAAACAGGATACTGTTGCGTACCTTATTGAAGAGGTTAATCTCTCCC
CTTCTTTATTAGCTGAAACAATTTCTATTGAATTTGCTGAACCATATTTTGATCTGGACGTTTACGATACTAGCCAGATT
CCTAAAGATTTGGTCGACCAGAAACTGATTTTAAAACATCGGATCTTGCCTCTCGTACAAAGAGGACAGATTTTATATGT
TGCAACCAGTAATCCCAGCAATATTGATGCTATTGATGCTATTCGCTTCAACAGCAAGCTTATGGTTGAGCCGGTTATTG
TCGAGCACCATAAACTCGAGAAAATTCTGGTACAGCAATTTACTGAAGAAAGTAATTTCGAGTTTAGCGATGAAGAGTTT
GATCTAGATGTAGATCTTGATACCTCAACTGCTCCAGAAGAGGATGAGGATACTCCGCAAGGCGATGAAGCACCTATTGT
TAAATATGTTAACAAGTTATTAATTGATGCGATTCGTATGGGGGCTTCTGATTTACATTTTGAACCTTATGAAAAATCGT
ATCGGGTCCGTTATCGGGTCGATGGCGTTTTGCGTCAAATTGCAAATCCGCCTTTGCAGCTGGCCAATCGTTTGGCTTCA
CGCTTAAAAGTGATGTCTCAAATGGACATTTCTGAAAAGCGTGTCCCTCAAGATGGTCGTATCAAGCTCAAGCTATCAAA
ATCTAAAGCAATTGATTTTCGTGTGAACTCTCTTCCGACCTTATTTGGTGAAAAGCTAGTTCTACGTATTCTCGATCCAT
CGAGTGCTATGCTGGGAATTGATGCTCTAGGATATGAAGAAGAGCAAAAAGCGCTCTTTATGGAAGCATTAGATAAGCCA
CAAGGTATGCTTTTAATCACTGGTCCGACAGGTTCTGGTAAGACTGTATCTTTATATACAGGCCTCAATATCTTAAATAC
TGAAAGTTCTAATATTTCTACTGCCGAAGATCCAGTCGAAATTAACCTTGAAGGCATTAATCAGGTTAACGTGAACCCTA
AGGTAGGTCTGACTTTTTCAGCTGCACTTAAGTCATTTTTACGTCAGGACCCTGACATTATTATGGTCGGTGAGATTCGT
GATTTAGAAACAGCTGAAATCGCGATTAAAGCGGCTCAGACAGGTCATATGGTGATGTCCACGCTACATACCAACAGTGC
GCCTGAAACACTGACTCGTCTACGCAATATGGGAGTTCCATCTTTCAATATTGCGACATCGGTCAATCTGGTCATTGCTC
AGCGTTTGGCACGTCGACTATGTTCTCAATGTAAAGTACCTACTGACATTCCTAAACAAAGCCTATTGGAAATGGGTTTT
ACCGAACAAGATCTGACACATCCCGATTTTCAAGTTTTTCAACCCGTCGGTTGTCCAGAATGTCGTGAGGGTTACAAAGG
TCGAGTTGGTATTTATGAAGTAATGAAAGTCACACCTGAGATTTCCAAGATTATTATGGAAGATGGCAATGCTTTAGAAA
TCGCTGCCGCTTCTGAAAAACTGGGGTTTAATAATCTACGTCGCTCAGGTTTAAAGAAAGTTATGCAAGGTGTTACTTCT
TTACAAGAAGTCAATCGCGTAACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-144)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

94.386

100

0.944
  pilB Acinetobacter baylyi ADP1

79.649

100

0.796
  pilB Legionella pneumophila strain ERS1305867

52.113

99.649

0.519
  pilF Neisseria gonorrhoeae MS11

48.932

98.596

0.482
  pilB Vibrio parahaemolyticus RIMD 2210633

51.711

92.281

0.477
  pilB Vibrio campbellii strain DS40M4

50.674

91.053

0.461
  pilB Vibrio cholerae strain A1552

50.096

91.053

0.456
  pilB/pilB1 Synechocystis sp. PCC 6803

35.505

100

0.382
  pilF Thermus thermophilus HB27

37.143

98.246

0.365