Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GZH91_RS03355 Genome accession   NZ_AP021884
Coordinates   637510..639225 (+) Length   571 a.a.
NCBI ID   WP_147070778.1    Uniprot ID   A0A512L4X6
Organism   Sulfuriferula plumbiphila strain Gro7     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 632510..644225
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GZH91_RS03330 (SFPGR_06630) rimM 633009..633524 (-) 516 WP_307723870.1 ribosome maturation factor RimM -
  GZH91_RS03335 (SFPGR_06640) rpsP 633499..633753 (-) 255 WP_147070771.1 30S ribosomal protein S16 -
  GZH91_RS03340 (SFPGR_06650) ffh 633838..635181 (-) 1344 WP_147070773.1 signal recognition particle protein -
  GZH91_RS03345 (SFPGR_06660) - 635229..636062 (+) 834 WP_223264475.1 inner membrane protein YpjD -
  GZH91_RS03350 (SFPGR_06670) - 636133..637422 (+) 1290 WP_147070776.1 HlyC/CorC family transporter -
  GZH91_RS03355 (SFPGR_06680) pilB 637510..639225 (+) 1716 WP_147070778.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GZH91_RS03360 (SFPGR_06690) pilC 639474..640703 (+) 1230 WP_147070780.1 type II secretion system F family protein Machinery gene
  GZH91_RS03365 (SFPGR_06700) pilD 640737..641606 (+) 870 WP_147070782.1 A24 family peptidase Machinery gene
  GZH91_RS03370 (SFPGR_06710) coaE 641609..642211 (+) 603 WP_147070784.1 dephospho-CoA kinase -
  GZH91_RS03375 (SFPGR_06720) zapD 642394..643146 (+) 753 WP_147070786.1 cell division protein ZapD -
  GZH91_RS03380 (SFPGR_06730) - 643300..643473 (+) 174 WP_147070787.1 Arm DNA-binding domain-containing protein -
  GZH91_RS03385 (SFPGR_06740) - 643508..643792 (+) 285 WP_147070789.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62675.30 Da        Isoelectric Point: 4.9968

>NTDB_id=75258 GZH91_RS03355 WP_147070778.1 637510..639225(+) (pilB) [Sulfuriferula plumbiphila strain Gro7]
MAATIANAGLTGLARALVHEKWLSEPEAEALAKQAASMGEGFVTQLINVKKIKPVQIAEFAAAQFGFPYFDLMALDLEVL
PKSLLDPKLVQKRRVLALYQRGNRLSVAVSDPTNLLAIDEVKFQTNLGVDIVVVEDDKLGKLIEQFSEGSASVLDSLDSE
DIDLEFADDDALAKVDEAASTDIDDAPIVRYLQKILLDAINTGVSDIHFEPFEKFYRIRYRLDGQLYEVAQPPIAIKEKI
SSRIKVISKLDIAEKRVPQDGRMKLVLSKNRAIDFRVSSLPTLYGEKIVMRILDPASAQLGIDALGYDPDQRELLLNAVQ
RPYGMVLVTGPTGSGKTVSLYTCLNILNKPGVNISTAEDPAEINLPGINQVNVNDKAGLTFAAALRAFLRQDPDIIMVGE
IRDLETADISIKAAQTGHMVLSTLHTNDAPSTLTRLMNMGVAPFNIASSVIMITAQRLARRLCSCKQPAIIPREALLRAG
FREEDLDGSWQPYRAVGCELCKNTGYKGRVGIYQVMPITEEMTRIIMKNGNAIDIADQARREGVRDLRQAGLLKVKAGMT
SLEEVEAVTNE

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=75258 GZH91_RS03355 WP_147070778.1 637510..639225(+) (pilB) [Sulfuriferula plumbiphila strain Gro7]
ATGGCTGCTACCATTGCTAATGCCGGCCTGACCGGTTTGGCGCGCGCGCTGGTTCACGAAAAATGGCTCTCCGAGCCGGA
GGCGGAGGCATTGGCCAAGCAGGCTGCGAGCATGGGCGAGGGGTTCGTCACCCAACTGATCAACGTCAAAAAAATCAAGC
CAGTGCAAATTGCCGAATTCGCTGCTGCGCAATTTGGTTTCCCGTATTTTGATCTGATGGCACTTGACCTTGAGGTCTTG
CCGAAAAGCCTGCTTGATCCCAAGCTGGTGCAAAAACGTCGTGTGCTTGCCCTCTATCAGCGCGGCAACCGGCTGTCGGT
TGCAGTATCTGATCCTACCAATCTGCTGGCCATTGACGAGGTCAAGTTCCAGACCAACCTCGGCGTGGATATTGTGGTGG
TGGAGGATGACAAGCTCGGCAAGCTGATCGAGCAGTTCAGCGAGGGTAGTGCCAGCGTACTGGACAGCCTCGATAGTGAA
GATATCGATCTGGAGTTTGCCGACGACGATGCGCTGGCCAAAGTGGACGAAGCGGCCAGTACGGATATTGACGATGCGCC
CATCGTCCGTTACCTGCAAAAAATCCTGCTGGATGCGATCAACACCGGCGTATCCGATATTCACTTTGAGCCATTCGAAA
AATTTTATCGCATCCGTTATCGCCTGGACGGACAGCTTTATGAAGTAGCCCAGCCACCGATAGCTATCAAGGAGAAAATC
TCTTCGCGCATCAAGGTGATCTCCAAACTCGATATTGCCGAAAAACGTGTGCCGCAGGACGGGCGCATGAAGCTGGTGCT
GTCCAAGAACCGCGCCATCGATTTCCGGGTGAGTTCGCTGCCCACGCTGTATGGCGAAAAAATCGTGATGCGAATTCTCG
ACCCGGCCAGTGCACAGCTCGGTATCGATGCGCTGGGCTACGACCCGGATCAAAGAGAGTTGTTGCTCAATGCCGTGCAG
CGCCCCTATGGCATGGTACTGGTAACCGGCCCCACCGGTAGTGGTAAAACCGTGTCGCTCTACACCTGCCTGAATATCCT
CAACAAGCCGGGGGTCAACATCTCCACGGCTGAAGATCCGGCGGAAATCAACCTGCCCGGCATCAATCAAGTCAACGTCA
ACGACAAGGCCGGACTGACCTTCGCTGCCGCGCTGCGTGCCTTCCTGCGCCAGGATCCGGACATCATCATGGTGGGCGAG
ATTCGCGACCTGGAGACCGCGGATATTTCCATCAAGGCCGCGCAAACCGGGCATATGGTGCTCTCCACCCTGCACACCAA
CGATGCGCCTTCCACGCTCACGCGCCTGATGAATATGGGCGTAGCGCCGTTCAATATTGCCTCCAGTGTGATCATGATTA
CCGCTCAGCGTCTGGCGCGGCGACTGTGCAGCTGCAAGCAGCCGGCTATCATTCCCAGGGAAGCGCTGCTGCGCGCCGGC
TTCAGGGAGGAAGACCTGGACGGTAGTTGGCAGCCATATCGTGCGGTGGGCTGCGAGCTATGCAAAAATACCGGCTACAA
GGGGCGGGTAGGCATCTACCAGGTAATGCCGATTACTGAAGAGATGACCCGCATCATCATGAAAAACGGCAACGCCATCG
ATATTGCAGACCAGGCGCGGCGTGAGGGTGTGCGCGATTTGCGTCAGGCCGGATTGCTCAAGGTCAAAGCCGGAATGACT
TCGCTGGAAGAAGTTGAAGCCGTAACCAATGAATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-149)

Type II/IV secretion system protein (T2SSE)

(194-461)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A512L4X6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.272

100

0.566
  pilB Acinetobacter baylyi ADP1

54.53

100

0.548
  pilB Legionella pneumophila strain ERS1305867

52.677

100

0.534
  pilF Neisseria gonorrhoeae MS11

54.174

98.599

0.534
  pilB Vibrio cholerae strain A1552

46.667

99.825

0.466
  pilB Vibrio parahaemolyticus RIMD 2210633

45.87

99.65

0.457
  pilB Vibrio campbellii strain DS40M4

45.614

99.825

0.455
  pilF Thermus thermophilus HB27

40.696

100

0.41
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.25

98.074

0.405
  pilB/pilB1 Synechocystis sp. PCC 6803

41.517

87.741

0.364

Multiple sequence alignment