Detailed information NTG0752534

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: OH720_RS18770
• Genome accession: NZ_CP110128
• Coordinates: 4187754..4188863 (-)
• Length: 369 a.a.
• NCBI ID: WP_272602406.1
• Uniprot ID: -
• Organism: Pseudomonas sp. WJP1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4182754..4193863

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OH720_RS18755 (OH720_18760)	-	4183312..4184703 (+)	1392	WP_180205459.1	GntP family permease	-
OH720_RS18760 (OH720_18765)	hbdH	4184711..4185484 (+)	774	WP_272602404.1	3-hydroxybutyrate dehydrogenase	-
OH720_RS18765 (OH720_18770)	-	4185674..4187629 (+)	1956	WP_272602405.1	acetoacetate--CoA ligase	-
OH720_RS18770 (OH720_18775)	pilU	4187754..4188863 (-)	1110	WP_272602406.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
OH720_RS18775 (OH720_18780)	-	4188984..4191521 (+)	2538	WP_272602407.1	PAS domain-containing protein	-
OH720_RS18780 (OH720_18785)	-	4191577..4191852 (+)	276	WP_007933743.1	peptidylprolyl isomerase	-
OH720_RS18785 (OH720_18790)	-	4191994..4192926 (-)	933	WP_272602408.1	sugar kinase	-

Sequence

Protein

• Length: 369 a.a.
• Molecular weight: 40680.35 Da
• Isoelectric Point: 5.9727

>NTDB_id=752534 OH720_RS18770 WP_272602406.1 4187754..4188863(-) (pilU) [Pseudomonas sp. WJP1]
MEIDALLSRLASQDGSDLYLSTGAPPSARFDGVLTALADRPFKPGEIAVIAASIMDAEQRLEFDRDLEMNLAISPPGIGR
FRVNIFKQRNDVSMVVRNVKLDIPRFEDLKLPSVLLETVMLKQGLMLFVGATDSGKSTSLAALIDYRNRHGSGHIVTIED
PIEYIHRHQRSIINQREVGVDTRSFHAALKNALRQAPDVVLIGEISDRETMEHALAFADSGHLVISTLHAHNASQALDRI
INFFPEERRGQLLNDLGNNLKAFVSQRLVRSVDGQRRAAVEVMLGTPTVGGLIRRNEFSELKGVMEKSVALGMQTFDGAL
FELVAEGVIDEEEALKHADSVNNLRLRLKLHGDTTRGSAQSSGDWGLMD

Nucleotide

• Length: 1110 bp

>NTDB_id=752534 OH720_RS18770 WP_272602406.1 4187754..4188863(-) (pilU) [Pseudomonas sp. WJP1]
ATGGAAATCGACGCGTTGTTAAGCCGCCTGGCCAGCCAGGATGGCTCTGATCTCTACCTGTCCACCGGCGCGCCGCCCAG
TGCGCGATTCGACGGAGTGCTCACCGCCCTGGCGGACCGACCGTTCAAGCCGGGAGAAATCGCTGTGATTGCTGCGTCCA
TCATGGACGCCGAACAGCGCCTTGAGTTCGATCGGGACCTGGAAATGAACCTGGCGATCTCCCCGCCCGGGATCGGACGC
TTTCGGGTGAACATCTTCAAGCAGCGTAACGATGTGTCGATGGTCGTGCGCAACGTCAAGCTCGACATACCACGCTTCGA
AGACCTCAAGTTGCCGTCGGTGTTGCTTGAAACGGTGATGCTCAAGCAAGGGCTGATGCTGTTCGTCGGCGCTACCGACT
CGGGCAAGTCTACCTCGCTGGCGGCGTTGATCGACTACCGTAATCGTCACGGTAGCGGCCACATCGTCACCATCGAAGAC
CCGATCGAGTACATCCACCGGCACCAGCGATCGATCATCAACCAGCGCGAAGTCGGTGTCGACACCCGCAGTTTTCATGC
TGCGTTGAAAAATGCCCTGCGCCAGGCGCCGGACGTGGTGCTGATCGGCGAGATCAGCGACCGCGAAACCATGGAGCATG
CCTTGGCGTTTGCCGATTCCGGGCACCTGGTGATCTCCACGCTGCACGCCCACAACGCCAGCCAGGCGCTCGATCGCATT
ATCAATTTTTTTCCTGAAGAGCGCCGGGGACAACTGCTTAACGATCTGGGCAACAACCTCAAGGCCTTTGTGTCGCAGCG
TCTGGTACGTAGCGTTGATGGCCAGCGGCGTGCTGCGGTAGAGGTGATGCTGGGAACGCCGACCGTTGGTGGCTTGATCC
GGCGTAATGAGTTTTCCGAGCTCAAAGGGGTGATGGAGAAGTCGGTGGCGCTGGGGATGCAAACGTTTGACGGGGCGCTG
TTCGAGTTGGTCGCAGAAGGTGTGATCGACGAGGAAGAAGCGTTGAAACATGCAGATTCGGTGAACAACCTGCGATTGCG
GTTGAAGTTGCACGGCGATACAACGCGAGGTTCTGCACAATCGTCAGGTGACTGGGGCTTGATGGATTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (113-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	54.348	99.729	0.542
pilU	Acinetobacter baylyi ADP1	51.136	95.393	0.488
pilU	Vibrio cholerae strain A1552	48.626	98.645	0.48
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	40.841	90.244	0.369
pilT	Vibrio cholerae strain A1552	40.841	90.244	0.369
pilT	Pseudomonas aeruginosa PAK	39.244	93.225	0.366
pilT	Pseudomonas stutzeri DSM 10701	39.244	93.225	0.366
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.06	89.973	0.36