Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   SFSGTM_RS14160 Genome accession   NZ_AP021881
Coordinates   2887783..2889501 (+) Length   572 a.a.
NCBI ID   WP_162085731.1    Uniprot ID   A0A809SAJ0
Organism   Sulfuriferula nivalis strain SGTM     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2882783..2894501
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SFSGTM_RS14140 (SFSGTM_27370) - 2882879..2885758 (-) 2880 WP_162085728.1 molybdopterin oxidoreductase family protein -
  SFSGTM_RS14145 (SFSGTM_27380) - 2885742..2885972 (-) 231 WP_162085729.1 hypothetical protein -
  SFSGTM_RS14150 (SFSGTM_27390) - 2886020..2886325 (+) 306 WP_162085730.1 hypothetical protein -
  SFSGTM_RS14155 (SFSGTM_27400) - 2886432..2887703 (+) 1272 WP_174237423.1 CNNM domain-containing protein -
  SFSGTM_RS14160 (SFSGTM_27410) pilB 2887783..2889501 (+) 1719 WP_162085731.1 type IV-A pilus assembly ATPase PilB Machinery gene
  SFSGTM_RS14165 (SFSGTM_27420) pilC 2889545..2890771 (+) 1227 WP_162085732.1 type II secretion system F family protein Machinery gene
  SFSGTM_RS14170 (SFSGTM_27430) pilD 2890774..2891628 (+) 855 WP_162085733.1 A24 family peptidase Machinery gene
  SFSGTM_RS14175 (SFSGTM_27440) coaE 2891628..2892230 (+) 603 WP_162085734.1 dephospho-CoA kinase -
  SFSGTM_RS14180 (SFSGTM_27450) zapD 2892250..2893056 (+) 807 WP_269780061.1 cell division protein ZapD -
  SFSGTM_RS14185 yacG 2893053..2893235 (+) 183 WP_162085735.1 DNA gyrase inhibitor YacG -
  SFSGTM_RS14190 (SFSGTM_27460) rpiA 2893232..2893900 (-) 669 WP_162085736.1 ribose-5-phosphate isomerase RpiA -

Sequence

Protein


Download         Length: 572 a.a.        Molecular weight: 62900.28 Da        Isoelectric Point: 5.2242

>NTDB_id=75246 SFSGTM_RS14160 WP_162085731.1 2887783..2889501(+) (pilB) [Sulfuriferula nivalis strain SGTM]
MASNSNPIILTGLARALVSQQWLTNDEAEAIMKDAKASNEQFVTQLLKSKKTTAVKLAEFAAVQFGFPYFDLNAIDPEVI
PNKLLDSKLIQSRRVVALYQRGTKLAVGLSDPTNLQAIDDIKFQTSLTVEVIVVEDDKLGKLITQISESSNNTIANLAGD
DDFELDLTDDATQNAKNAEESQEIDDAPIVRYLQKILLDAINDGVSDIHFEPYEKYYRIRYRLDGVLREIAQPPLAIKDK
LASRIKVISKLDISEKRVPQDGRMKLVLSKNRAIDFRVSTLPTLYGEKIVMRILDPTSATLGIEALGYEPKQKELLLNAV
QRPYGMVLVTGPTGSGKTVSLYTCLNILNQPGVNIATAEDPAEINLPGINQVNVNDKAGLTFSAALKSFLRQDPDIIMVG
EIRDLETAEIAIKAAQTGHMVLSTLHTNDAPSTLTRLMNMGIAPFNIASSVIMITAQRLARKLCKCKQPIDIPKEALLRA
GFAESELDGSWKPYHAVGCDLCKGTGYKGRLGIYQVMPITDEMRRIIMSNGNAIDIADQARREGVSDLRRSGLNKVITGL
TSLEEVESVTNE

Nucleotide


Download         Length: 1719 bp        

>NTDB_id=75246 SFSGTM_RS14160 WP_162085731.1 2887783..2889501(+) (pilB) [Sulfuriferula nivalis strain SGTM]
ATGGCATCAAACTCAAACCCCATCATATTGACAGGTCTTGCGCGCGCACTAGTTAGCCAACAATGGCTCACCAATGACGA
GGCAGAGGCCATTATGAAAGACGCCAAAGCCAGCAATGAACAATTCGTCACCCAGCTGCTAAAATCCAAGAAAACTACGG
CCGTTAAATTAGCTGAGTTTGCGGCAGTACAATTTGGTTTTCCTTATTTTGATTTAAATGCGATAGACCCTGAAGTCATA
CCCAACAAACTGTTAGACAGCAAACTGATACAAAGTCGTCGCGTCGTTGCACTATATCAAAGAGGTACCAAATTAGCGGT
TGGGCTGTCCGACCCAACCAATTTACAAGCCATCGATGACATAAAGTTTCAAACATCACTGACTGTTGAAGTTATCGTTG
TTGAAGATGACAAACTTGGCAAACTCATTACTCAAATTAGTGAAAGCAGTAACAACACCATCGCCAATTTGGCGGGTGAC
GATGATTTTGAACTTGATCTTACTGACGACGCTACACAAAATGCAAAAAATGCTGAAGAATCCCAAGAAATCGATGACGC
TCCAATTGTTCGGTATCTGCAAAAAATACTCTTAGATGCTATCAATGATGGTGTATCTGACATCCATTTCGAGCCCTACG
AAAAATATTACCGCATACGCTACCGATTAGACGGTGTGCTACGCGAGATCGCGCAGCCTCCATTAGCAATTAAAGACAAA
CTGGCTTCCCGCATTAAGGTCATCTCGAAACTCGACATTTCAGAAAAACGCGTCCCACAAGACGGGCGCATGAAACTCGT
GTTATCAAAAAACCGCGCAATCGATTTCCGCGTCAGCACCCTACCCACCCTGTATGGTGAAAAAATTGTGATGCGTATTC
TTGACCCAACCAGTGCAACATTGGGTATTGAAGCATTAGGATATGAACCCAAGCAAAAAGAGCTGCTGCTAAACGCAGTA
CAACGCCCCTATGGCATGGTGCTCGTGACCGGGCCAACAGGTAGTGGTAAAACTGTCTCGCTGTACACCTGCTTAAACAT
CCTGAATCAGCCGGGCGTCAACATCGCTACAGCAGAAGATCCAGCTGAAATTAACTTGCCTGGCATAAACCAAGTTAACG
TGAATGATAAAGCGGGCTTAACCTTTTCTGCCGCATTAAAGTCGTTTTTGCGACAAGACCCAGACATCATCATGGTCGGT
GAAATACGCGATCTGGAAACAGCTGAAATCGCGATTAAAGCCGCACAAACAGGTCACATGGTGCTATCAACACTGCATAC
AAATGATGCACCATCCACATTGACCCGTTTAATGAATATGGGAATCGCTCCATTTAACATAGCATCCAGCGTCATCATGA
TTACCGCGCAGCGACTAGCTCGGAAATTATGCAAGTGCAAACAACCTATAGACATACCAAAGGAAGCCTTGTTAAGAGCT
GGGTTTGCTGAGAGCGAGCTTGATGGTAGCTGGAAACCCTATCATGCTGTTGGTTGCGATCTTTGCAAAGGTACAGGATA
CAAAGGAAGGCTAGGCATTTACCAAGTCATGCCCATAACTGATGAAATGCGCCGCATCATTATGAGCAACGGCAATGCAA
TTGACATTGCCGACCAGGCGCGCCGCGAAGGTGTGAGCGACTTGAGGCGCTCTGGTCTAAATAAGGTCATTACAGGACTG
ACCTCACTAGAAGAAGTTGAGTCAGTTACTAACGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(195-462)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(41-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A809SAJ0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.89

98.951

0.563
  pilB Acinetobacter baylyi ADP1

53.993

100

0.544
  pilF Neisseria gonorrhoeae MS11

54.69

98.776

0.54
  pilB Legionella pneumophila strain ERS1305867

53.299

100

0.537
  pilB Vibrio cholerae strain A1552

47.387

100

0.476
  pilB Vibrio campbellii strain DS40M4

45.819

100

0.46
  pilB Vibrio parahaemolyticus RIMD 2210633

46.797

98.252

0.46
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.177

98.776

0.397
  pilF Thermus thermophilus HB27

38.581

100

0.39
  pilB/pilB1 Synechocystis sp. PCC 6803

38.043

96.503

0.367

Multiple sequence alignment