Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   D3P44_RS16545 Genome accession   NZ_CP110110
Coordinates   3474274..3475977 (-) Length   567 a.a.
NCBI ID   WP_212542967.1    Uniprot ID   -
Organism   Stutzerimonas balearica strain Z8     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3469274..3480977
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D3P44_RS16510 (D3P44_016510) rpsI 3469383..3469775 (-) 393 WP_043221690.1 30S ribosomal protein S9 -
  D3P44_RS16515 (D3P44_016515) rplM 3469790..3470218 (-) 429 WP_041104003.1 50S ribosomal protein L13 -
  D3P44_RS16520 (D3P44_016520) - 3470585..3471295 (+) 711 WP_043221692.1 energy-coupling factor ABC transporter permease -
  D3P44_RS16525 (D3P44_016525) yacG 3471289..3471486 (-) 198 WP_074519929.1 DNA gyrase inhibitor YacG -
  D3P44_RS16530 (D3P44_016530) coaE 3471483..3472091 (-) 609 WP_212542969.1 dephospho-CoA kinase -
  D3P44_RS16535 (D3P44_016535) pilD 3472185..3473054 (-) 870 WP_212542968.1 prepilin peptidase Machinery gene
  D3P44_RS16540 (D3P44_016540) pilC 3473051..3474271 (-) 1221 WP_061340859.1 type II secretion system F family protein Machinery gene
  D3P44_RS16545 (D3P44_016545) pilB 3474274..3475977 (-) 1704 WP_212542967.1 type IV-A pilus assembly ATPase PilB Machinery gene
  D3P44_RS20940 - 3476400..3476585 (+) 186 WP_212542966.1 type IV pilin protein -
  D3P44_RS16555 (D3P44_016555) - 3476605..3477006 (+) 402 WP_212542965.1 hypothetical protein -
  D3P44_RS16560 (D3P44_016560) - 3477152..3478168 (+) 1017 WP_032728035.1 IS30-like element IS1394 family transposase -
  D3P44_RS16565 (D3P44_016565) - 3478526..3479230 (+) 705 WP_249345724.1 ABC transporter permease subunit -
  D3P44_RS16570 (D3P44_016570) - 3479232..3480068 (+) 837 WP_212739882.1 hypothetical protein -
  D3P44_RS16575 (D3P44_016575) - 3480065..3480961 (+) 897 WP_212739883.1 ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62186.12 Da        Isoelectric Point: 5.6181

>NTDB_id=752427 D3P44_RS16545 WP_212542967.1 3474274..3475977(-) (pilB) [Stutzerimonas balearica strain Z8]
MNENAPLGGLARQLVQVGQLDEKTAQQAQQQALRNQVPLVTWLVQNKLVKGRVLVDIAGEQFGVPFFDLSALDKDSQPKD
LVSEKLVRQHRALPLQRRGNKLYVAVSDPTNHQAISDIQFNTGLSVEAVLVEDDRLGEAIEKFFESATSSLDGLTDSELG
GLDIEVAGEAKEDDAAHNEADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKSYRVRFRTDGMLHEVARPPVQLVSRISAR
LKVMASLDIAERRKPQDGRIKMKVSSTKSVDFRVNTLPTLWGEKIVMRILDSASAKMGIDALGYEDDQKELYLSALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTVDVNISTAEDPVEINLEGINQVNVNPRQGMDFAQALRAFLRQDPDVIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPAFNIATSVNLIIAQRLARKLCPSCKAEVDVPREVLLKEGFP
EARIGTFKLYGPVGCDSCKGGYKGRVGIYEVVKITPSLQRLIMEEGNSIDIALQARKDGFNDLRTSGLVKAMNGVTSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=752427 D3P44_RS16545 WP_212542967.1 3474274..3475977(-) (pilB) [Stutzerimonas balearica strain Z8]
ATGAACGAGAACGCTCCCCTCGGCGGCCTGGCCCGGCAGCTTGTGCAGGTCGGCCAGCTTGACGAAAAAACCGCGCAGCA
AGCGCAGCAGCAGGCTTTGCGGAATCAGGTTCCGTTGGTGACTTGGTTGGTGCAGAACAAGTTGGTCAAAGGGCGGGTGC
TCGTTGACATCGCCGGCGAGCAGTTCGGCGTGCCGTTCTTCGATCTGAGTGCGCTAGACAAGGATTCGCAGCCAAAGGAC
CTGGTCAGCGAAAAGCTGGTTCGCCAGCACCGCGCATTGCCGCTTCAGCGGCGCGGCAACAAGCTTTATGTCGCCGTGTC
GGATCCGACCAATCATCAGGCTATCAGCGATATCCAGTTCAACACCGGCCTCTCCGTCGAGGCCGTACTGGTTGAGGACG
ACAGGCTCGGCGAGGCGATCGAGAAGTTTTTCGAGTCAGCGACTTCCTCCCTCGATGGGTTGACCGATTCCGAGTTGGGC
GGCTTGGATATCGAGGTTGCGGGTGAGGCCAAGGAAGATGATGCAGCGCATAACGAGGCCGATGATGCGCCGGTGGTGAG
ATTCGTCAACAAGATGCTGCTGGATGCAATAAAGGGCGGCTCATCAGATTTGCACTTTGAACCTTATGAAAAGAGCTATC
GGGTTCGTTTTCGTACGGATGGCATGTTGCATGAGGTCGCCCGCCCACCAGTGCAGTTGGTCAGTCGCATTTCAGCACGG
TTGAAGGTGATGGCCAGTCTGGATATCGCCGAACGACGCAAGCCCCAGGATGGGCGCATCAAGATGAAGGTGTCGAGCAC
CAAATCCGTCGATTTCCGGGTGAATACGCTTCCGACCCTCTGGGGCGAAAAGATCGTTATGCGAATCCTCGACTCGGCGA
GTGCCAAGATGGGTATTGATGCGCTGGGCTACGAGGATGACCAGAAGGAGCTGTACCTGAGCGCGCTTAAGCAGCCGCAA
GGAATGATCCTCGTTACGGGGCCGACCGGTTCCGGCAAGACCGTTTCCCTTTACACCGGCCTGAACATCCTCAATACGGT
CGACGTGAACATCTCAACGGCCGAGGATCCGGTGGAAATCAACCTCGAGGGGATCAATCAGGTCAACGTTAACCCCAGGC
AGGGCATGGACTTCGCACAGGCCCTGCGTGCGTTCCTGCGCCAAGACCCGGACGTGATCATGGTCGGTGAGATTCGCGAT
CTGGAAACTGCCGAGATCGCGATCAAGGCGGCACAGACCGGCCACATGGTGATGTCGACGCTGCATACCAATAGTGCGGC
AGAGACCCTGACGCGGTTGCGCAACATGGGAGTGCCGGCTTTCAACATCGCGACCTCGGTGAACTTGATCATTGCTCAGC
GGCTGGCGCGAAAACTTTGCCCGAGCTGCAAGGCGGAAGTTGATGTGCCGCGTGAGGTATTGCTCAAGGAGGGCTTCCCG
GAAGCTCGGATAGGCACCTTCAAGCTCTACGGGCCGGTTGGTTGCGACAGTTGCAAGGGGGGCTACAAGGGGCGTGTCGG
TATTTATGAAGTGGTTAAAATCACGCCCTCGCTGCAGCGACTTATCATGGAAGAAGGAAATTCCATCGATATCGCACTTC
AGGCGCGCAAGGATGGCTTCAACGACCTGCGCACCTCTGGCTTGGTCAAGGCCATGAATGGCGTAACCAGTCTGGAAGAA
GTCAACCGCGTCACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-148)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

59.431

99.118

0.589
  pilB Acinetobacter baylyi ADP1

57.876

99.647

0.577
  pilB Legionella pneumophila strain ERS1305867

54.291

100

0.547
  pilF Neisseria gonorrhoeae MS11

49.735

99.647

0.496
  pilB Vibrio campbellii strain DS40M4

49.381

99.647

0.492
  pilB Vibrio parahaemolyticus RIMD 2210633

53.635

89.771

0.481
  pilB Vibrio cholerae strain A1552

53.242

89.771

0.478
  pilF Thermus thermophilus HB27

38.366

99.295

0.381
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.454

93.298

0.377
  pilB/pilB1 Synechocystis sp. PCC 6803

40.577

91.711

0.372