Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   MPB2EB_RS01030 Genome accession   NZ_AP021872
Coordinates   239626..240921 (-) Length   431 a.a.
NCBI ID   WP_185182042.1    Uniprot ID   -
Organism   Mycoavidus sp. B2-EB     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 234626..245921
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MPB2EB_RS01000 (MPB2EB_0200) - 234954..235406 (+) 453 WP_185182036.1 NUDIX domain-containing protein -
  MPB2EB_RS01005 (MPB2EB_0201) - 235426..235614 (-) 189 WP_185182037.1 DNA gyrase inhibitor YacG -
  MPB2EB_RS01010 (MPB2EB_0202) zapD 235651..236409 (-) 759 WP_185182038.1 cell division protein ZapD -
  MPB2EB_RS01015 (MPB2EB_0203) coaE 236586..237209 (-) 624 WP_185182039.1 dephospho-CoA kinase -
  MPB2EB_RS01020 (MPB2EB_0205) - 237397..238314 (-) 918 WP_185182040.1 A24 family peptidase -
  MPB2EB_RS01025 (MPB2EB_0206) - 238389..239615 (-) 1227 WP_185182041.1 type II secretion system F family protein -
  MPB2EB_RS01030 (MPB2EB_0207) pilF 239626..240921 (-) 1296 WP_185182042.1 GspE/PulE family protein Machinery gene
  MPB2EB_RS01035 (MPB2EB_0208) - 241116..242408 (-) 1293 WP_185182043.1 HlyC/CorC family transporter -
  MPB2EB_RS01040 (MPB2EB_0209) - 242519..243508 (-) 990 WP_370576621.1 polyprenyl synthetase family protein -
  MPB2EB_RS01045 (MPB2EB_0210) - 243581..244390 (-) 810 WP_198422338.1 cytochrome c oxidase assembly protein -

Sequence

Protein


Download         Length: 431 a.a.        Molecular weight: 47056.24 Da        Isoelectric Point: 8.0731

>NTDB_id=75127 MPB2EB_RS01030 WP_185182042.1 239626..240921(-) (pilF) [Mycoavidus sp. B2-EB]
MTAHPGQILLQTTDINPPWNSATTAPGSLSSEAENNAPAVRLLREILRAASTRLASDIHIESNEHDWRIRLRIDGVLHET
MRPPPYLRDPLICCVKVLAHLDIAERRIPQDGRLKLHVTAEKFEEFRVNTLPTLWGEKLVLRRLDTLPAALTFTSLGFNP
EQQAKLEAAIHAPHGMVLVTGPTGSGKTLSLYCFLQRLNRESLNICSVEDPVEIQLVGINQVSVREKTGLTFAVALRAFL
RQDPDVIMVGEIRDAETASVALQAAQTGHLVFSTLHTNNAPATLARLLDIGIAPFNLACAIRLIIAQRLIRKLCLACRAP
ISRNTTTDAMLYRAGFDKATLTQAWQAYRAVGCAACHNTGYRGRIGIYQVMPVTAAIRTLILAQGSADAIAQQAQRDGVK
TLREAGLEQVHAGTTSLEEVLAVTDASAATF

Nucleotide


Download         Length: 1296 bp        

>NTDB_id=75127 MPB2EB_RS01030 WP_185182042.1 239626..240921(-) (pilF) [Mycoavidus sp. B2-EB]
ATGACCGCTCATCCAGGCCAAATTCTGCTGCAAACAACAGATATAAATCCACCTTGGAACTCAGCAACTACAGCACCAGG
CTCACTTTCATCTGAAGCAGAGAATAATGCTCCGGCCGTGCGCTTATTGCGCGAAATTTTGCGCGCAGCCTCCACCCGGC
TCGCCTCTGATATACATATTGAATCAAACGAGCATGATTGGCGCATTCGACTCCGCATCGATGGCGTACTGCATGAAACC
ATGCGGCCCCCACCCTATCTAAGAGACCCGCTTATCTGTTGCGTGAAAGTACTCGCTCATCTCGATATCGCCGAGCGCCG
CATCCCTCAAGACGGGCGCCTCAAACTACACGTCACAGCAGAAAAATTTGAAGAATTCCGGGTGAATACGCTACCCACTT
TATGGGGCGAAAAACTCGTCTTGCGGCGACTGGATACACTTCCAGCAGCGCTAACTTTCACTAGCCTTGGCTTTAACCCC
GAACAACAGGCTAAACTTGAAGCCGCAATTCACGCCCCGCATGGCATGGTATTGGTGACTGGCCCAACTGGAAGCGGTAA
AACTTTATCGCTCTATTGTTTTTTACAACGCTTAAATCGAGAATCACTGAATATCTGCTCCGTCGAAGACCCGGTAGAAA
TACAATTAGTCGGCATTAATCAGGTCAGCGTGCGCGAAAAAACCGGCCTCACTTTTGCTGTCGCGCTGCGCGCTTTTTTA
CGACAAGATCCTGATGTCATTATGGTTGGAGAAATTCGCGATGCTGAAACTGCTAGCGTTGCGCTACAAGCCGCACAAAC
TGGCCACCTTGTCTTTTCTACGCTGCATACGAATAATGCTCCAGCCACACTCGCGCGCTTGCTTGATATTGGCATTGCGC
CTTTCAACTTGGCGTGCGCTATACGCTTAATTATCGCGCAAAGGCTCATCAGAAAACTTTGCTTAGCCTGCCGCGCTCCC
ATTTCCCGCAATACAACTACTGATGCAATGTTATATAGAGCGGGCTTTGACAAGGCAACCCTAACGCAAGCTTGGCAAGC
TTATCGAGCCGTTGGCTGCGCGGCATGTCATAACACAGGTTATCGTGGCCGCATCGGCATCTATCAAGTTATGCCGGTTA
CGGCCGCTATCCGCACACTTATCCTCGCCCAAGGCAGCGCAGATGCGATTGCCCAACAAGCGCAGCGCGACGGCGTTAAA
ACCTTACGCGAAGCGGGTCTAGAACAGGTTCACGCTGGCACCACCAGTCTTGAAGAAGTTTTAGCCGTAACCGATGCGAG
TGCAGCGACTTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(48-312)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

52.01

92.343

0.48
  pilB Acinetobacter baylyi ADP1

51.414

90.255

0.464
  pilB Legionella pneumophila strain ERS1305867

50.769

90.487

0.459
  pilB Acinetobacter baumannii D1279779

50.128

90.719

0.455
  pilB Vibrio cholerae strain A1552

48.601

91.183

0.443
  pilB Vibrio campbellii strain DS40M4

48.196

90.023

0.434
  pilB Vibrio parahaemolyticus RIMD 2210633

46.717

91.879

0.429
  pilB/pilB1 Synechocystis sp. PCC 6803

42.054

94.896

0.399
  pilF Thermus thermophilus HB27

43.59

90.487

0.394
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.199

90.719

0.383
  pilB Haemophilus influenzae Rd KW20

41.645

90.255

0.376
  pilB Haemophilus influenzae 86-028NP

41.131

90.255

0.371

Multiple sequence alignment