Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   OF801_RS00850 Genome accession   NZ_CP109635
Coordinates   172333..173277 (+) Length   314 a.a.
NCBI ID   WP_264308683.1    Uniprot ID   A0AA46TXL4
Organism   Lactococcus garvieae strain Alexii-11_2     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 167333..178277
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OF801_RS00835 (OF801_00835) - 168741..170195 (-) 1455 WP_264308318.1 GH25 family lysozyme -
  OF801_RS00840 (OF801_00840) guaB 170364..171845 (+) 1482 WP_003133714.1 IMP dehydrogenase -
  OF801_RS00845 (OF801_00845) rpsU 171968..172144 (+) 177 WP_003134539.1 30S ribosomal protein S21 -
  OF801_RS00850 (OF801_00850) comGA 172333..173277 (+) 945 WP_264308683.1 competence type IV pilus ATPase ComGA Machinery gene
  OF801_RS00855 (OF801_00855) comGB 173216..174235 (+) 1020 WP_264308684.1 competence type IV pilus assembly protein ComGB Machinery gene
  OF801_RS00860 (OF801_00860) comYC 174254..174559 (+) 306 WP_016171026.1 competence type IV pilus major pilin ComGC Machinery gene
  OF801_RS00865 (OF801_00865) comGD 174534..174950 (+) 417 WP_264308320.1 competence type IV pilus minor pilin ComGD -
  OF801_RS00870 (OF801_00870) comGE 174982..175215 (+) 234 WP_229031266.1 competence type IV pilus minor pilin ComGE -
  OF801_RS00875 (OF801_00875) comGF 175196..175636 (+) 441 WP_338129513.1 competence type IV pilus minor pilin ComGF -
  OF801_RS00880 (OF801_00880) - 175645..175806 (+) 162 WP_264308322.1 hypothetical protein -
  OF801_RS00885 (OF801_00885) - 175869..176714 (+) 846 WP_003133722.1 zinc ABC transporter substrate-binding protein -
  OF801_RS00890 (OF801_00890) - 176800..177669 (-) 870 WP_264308323.1 RluA family pseudouridine synthase -

Sequence

Protein


Download         Length: 314 a.a.        Molecular weight: 35836.02 Da        Isoelectric Point: 6.5037

>NTDB_id=748626 OF801_RS00850 WP_264308683.1 172333..173277(+) (comGA) [Lactococcus garvieae strain Alexii-11_2]
MIQVLAKTLLQKAVDQMVHDLYFVALEGKYSLYFRTATERRFERELDLEQGQALIAHMKFLSGMNLGESRRVQLGACTYV
LDKGEQRLRLSTVGDFHGQESLVIRLLHHQQNQLHFWNSEIFNTFIGGRGLYLFSGPVGSGKTSLMYKFAREHFKNQQVI
CIEDPVELVETEFLQLQVNKVIGNDYDALIKLSLRHRPDLLIVGEIRDSQTAKAVLRASLTGYTVFSTVHARSISGVIAR
LKELGLTDWELQSSLQRVIYQRLIAGKGLLVYEKEKFEEWQPDEWNGQIDQLVADGFISAVTAAHEKIEFSEAD

Nucleotide


Download         Length: 945 bp        

>NTDB_id=748626 OF801_RS00850 WP_264308683.1 172333..173277(+) (comGA) [Lactococcus garvieae strain Alexii-11_2]
ATGATACAGGTTTTGGCAAAAACCCTTTTACAAAAAGCAGTAGATCAGATGGTGCATGATCTTTATTTCGTAGCATTAGA
AGGAAAGTATTCTCTCTATTTTCGTACAGCAACAGAAAGGAGGTTTGAAAGAGAACTTGATCTTGAACAAGGGCAAGCAC
TGATTGCTCATATGAAATTTTTATCTGGTATGAATCTAGGAGAAAGTAGGCGGGTACAGTTGGGGGCTTGCACTTATGTT
TTAGATAAAGGTGAGCAGCGTTTACGTTTATCTACAGTCGGTGATTTTCATGGTCAAGAAAGCTTGGTTATCCGACTTCT
GCATCATCAACAAAACCAACTTCATTTTTGGAATTCAGAGATTTTTAATACTTTTATTGGTGGAAGAGGGCTTTACCTGT
TTTCCGGTCCCGTAGGCTCAGGTAAGACCAGCCTTATGTACAAGTTTGCAAGGGAGCATTTTAAAAATCAACAAGTTATT
TGTATAGAAGATCCCGTGGAGTTAGTGGAAACTGAGTTTTTACAACTTCAAGTCAATAAAGTAATAGGGAATGATTACGA
TGCCTTGATTAAGCTCTCTCTTCGCCACAGACCTGATTTACTCATTGTTGGTGAAATTAGGGACAGTCAAACGGCAAAAG
CTGTTCTCCGCGCAAGTTTGACGGGATATACAGTGTTTTCTACTGTTCATGCACGCTCAATATCGGGTGTAATTGCACGG
CTAAAGGAACTTGGGTTAACTGATTGGGAGCTACAATCCAGCCTTCAGCGGGTGATTTATCAGCGCTTAATTGCAGGAAA
GGGGTTGCTTGTTTATGAAAAAGAAAAGTTTGAAGAATGGCAACCAGACGAATGGAATGGCCAGATTGATCAGTTGGTTG
CAGATGGATTTATCTCAGCTGTTACAGCTGCGCACGAAAAAATTGAGTTTAGCGAAGCAGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-264)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Lactococcus lactis subsp. cremoris KW2

59.554

100

0.596
  comYA Streptococcus gordonii str. Challis substr. CH1

54.114

100

0.545
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

53.355

99.682

0.532
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

53.035

99.682

0.529
  comGA/cglA/cilD Streptococcus pneumoniae D39

53.035

99.682

0.529
  comGA/cglA/cilD Streptococcus pneumoniae R6

53.035

99.682

0.529
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

53.035

99.682

0.529
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

52.09

99.045

0.516
  comYA Streptococcus mutans UA159

50.949

100

0.513
  comYA Streptococcus mutans UA140

50.949

100

0.513