Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   OGV21_RS26930 Genome accession   NZ_CP109606
Coordinates   5933720..5934982 (-) Length   420 a.a.
NCBI ID   WP_264316631.1    Uniprot ID   -
Organism   Pseudomonas putida strain QH1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5928720..5939982
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OGV21_RS26910 - 5930030..5930254 (-) 225 WP_141729057.1 hypothetical protein -
  OGV21_RS26915 - 5930384..5931988 (-) 1605 WP_012274629.1 AAA family ATPase -
  OGV21_RS26920 aroB 5932004..5933101 (-) 1098 WP_041166613.1 3-dehydroquinate synthase -
  OGV21_RS26925 aroK 5933197..5933715 (-) 519 WP_041166614.1 shikimate kinase AroK -
  OGV21_RS26930 pilQ 5933720..5934982 (-) 1263 WP_264316631.1 type IV pilus secretin PilQ Machinery gene
  OGV21_RS26935 - 5934912..5935922 (-) 1011 WP_012274633.1 pilus assembly protein PilP -
  OGV21_RS26940 - 5935919..5936455 (-) 537 WP_012274634.1 PilN domain-containing protein -
  OGV21_RS26945 pilM 5936452..5937333 (-) 882 WP_012274635.1 type IV pilus biogenesis protein PilM -
  OGV21_RS26950 - 5937515..5939965 (+) 2451 WP_370739375.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 420 a.a.        Molecular weight: 45572.22 Da        Isoelectric Point: 4.9848

>NTDB_id=748452 OGV21_RS26930 WP_264316631.1 5933720..5934982(-) (pilQ) [Pseudomonas putida strain QH1]
MKTWMKYLLATLLSVPLASATSYEGEPLSLNFQDVEVRAVLQVLADYAGVNLVASDAVQGNVTMRLQDVPWDQALDLVLR
SKGLARRQEGNVLLVAPAADFAAQSAETRAGQVLEAQLQPLRRELLPIHHAKAADLAELLQASLVGDGILTGSLSVDERT
NTLVVHLPAGRLAELRQLVTQLDVPVRQVAIEARIVEANVDYEKSLGVRWGGPLYGAQLRPGKELFVDLGVERAGGSIGL
GLLRGDVLLDLELSAMEKSGNGEIISQPKVVTADKETARILKGTEVPYQETSQSGATSVSFREASLSLEVTPQITPDNKV
IMTVRVTKDEPDYVNALNNVPPIRKNEVNAKVRVADGETIVIGGVYSTSQNNVVDKVPFFGDLPYVGRLFRRDALQEKKS
ELLVFLTPRIMSDQAIAVSR

Nucleotide


Download         Length: 1263 bp        

>NTDB_id=748452 OGV21_RS26930 WP_264316631.1 5933720..5934982(-) (pilQ) [Pseudomonas putida strain QH1]
ATGAAAACGTGGATGAAATATCTGCTGGCAACCCTGCTGTCGGTTCCGCTGGCGTCGGCGACGTCTTATGAGGGTGAGCC
GCTATCGTTGAACTTTCAGGATGTAGAGGTGCGCGCGGTACTGCAGGTACTGGCGGACTATGCCGGGGTAAACTTGGTAG
CCAGCGATGCGGTGCAAGGCAACGTCACGATGCGCTTGCAGGACGTGCCGTGGGATCAGGCACTGGACCTGGTGTTGCGC
AGCAAGGGCCTGGCTCGGCGCCAGGAGGGTAATGTGCTGTTGGTGGCGCCTGCCGCCGACTTTGCCGCACAGTCAGCCGA
GACCCGTGCGGGGCAAGTGCTGGAAGCGCAGTTGCAACCTTTGCGTCGGGAACTGCTGCCTATTCACCACGCCAAGGCTG
CCGATTTGGCGGAGTTGCTACAGGCGAGCCTGGTGGGTGACGGTATTCTCACGGGTAGCCTGAGTGTCGATGAGCGCACC
AATACCCTGGTGGTGCACCTGCCTGCCGGCCGCCTGGCCGAATTGCGGCAACTGGTGACGCAGTTGGATGTGCCGGTGCG
CCAGGTAGCGATCGAGGCGCGTATCGTCGAGGCCAACGTCGATTACGAAAAGAGCCTGGGGGTGCGCTGGGGCGGGCCGC
TATACGGCGCGCAACTGCGGCCAGGCAAAGAATTGTTCGTCGATCTCGGCGTCGAGCGTGCCGGCGGCAGTATCGGCCTT
GGGTTATTGCGCGGGGACGTGTTGCTGGACCTTGAGCTCAGCGCCATGGAAAAGAGTGGTAACGGCGAGATCATTTCGCA
GCCCAAGGTGGTCACGGCCGACAAGGAAACGGCGAGGATCCTCAAGGGAACCGAGGTGCCTTACCAGGAAACCAGCCAAA
GCGGTGCGACCTCGGTATCTTTTCGCGAAGCGTCGCTGTCGTTGGAAGTTACCCCGCAGATCACCCCGGACAATAAGGTG
ATCATGACCGTCAGGGTGACCAAGGACGAGCCGGATTACGTCAATGCCTTGAACAACGTACCGCCGATCCGCAAGAACGA
GGTAAATGCCAAGGTTCGTGTGGCTGATGGCGAAACGATCGTGATTGGTGGCGTTTACTCGACTTCGCAAAACAATGTGG
TCGACAAGGTGCCATTTTTCGGCGATCTGCCGTATGTTGGGCGGCTGTTTCGACGCGATGCATTACAAGAGAAAAAATCC
GAGCTGCTGGTCTTCCTGACTCCGCGTATCATGAGTGACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(51-97)

Bacterial type II/III secretion system short domain (Secretin_N)

(125-188)

Bacterial type II and III secretion system protein (Secretin)

(255-411)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

59.132

100

0.617
  pilQ Acinetobacter baumannii D1279779

41.595

100

0.46
  pilQ Acinetobacter baumannii strain A118

41.595

100

0.46
  comQ Acinetobacter baylyi ADP1

42.699

100

0.46
  pilQ Legionella pneumophila strain ERS1305867

43.914

99.762

0.438
  pilQ Legionella pneumophila strain Lp02

43.914

99.762

0.438
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

41.745

100

0.421
  pilQ Vibrio cholerae strain A1552

41.745

100

0.421
  pilQ Vibrio campbellii strain DS40M4

40.553

100

0.419