Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   OGV19_RS25515 Genome accession   NZ_CP109603
Coordinates   5646057..5647325 (+) Length   422 a.a.
NCBI ID   WP_264311197.1    Uniprot ID   -
Organism   Pseudomonas putida strain SEH01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5641057..5652325
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OGV19_RS25495 - 5641092..5643542 (-) 2451 WP_413470149.1 penicillin-binding protein 1A -
  OGV19_RS25500 pilM 5643724..5644587 (+) 864 WP_264311194.1 type IV pilus biogenesis protein PilM -
  OGV19_RS25505 - 5644584..5645120 (+) 537 WP_264311195.1 PilN domain-containing protein -
  OGV19_RS25510 - 5645135..5646133 (+) 999 WP_264311196.1 pilus assembly protein PilP -
  OGV19_RS25515 pilQ 5646057..5647325 (+) 1269 WP_264311197.1 type IV pilus secretin PilQ Machinery gene
  OGV19_RS25520 aroK 5647330..5647848 (+) 519 WP_028698445.1 shikimate kinase AroK -
  OGV19_RS25525 aroB 5647945..5649042 (+) 1098 WP_264311198.1 3-dehydroquinate synthase -
  OGV19_RS25530 - 5649058..5650695 (+) 1638 WP_264311199.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 422 a.a.        Molecular weight: 46051.88 Da        Isoelectric Point: 5.3756

>NTDB_id=748403 OGV19_RS25515 WP_264311197.1 5646057..5647325(+) (pilQ) [Pseudomonas putida strain SEH01]
MKRLWKWVAAFALSIPIASATSYRGEPLSLNFQDVQVRAVLQVLADYAGVNLVASDSVQGSITLRLQDVPWDQALDLVLR
SKGLARHEEGNVLLVAPAAELAGQSLDARVGQVLDAQLQPLRRELMPIHHAKASELAELLLATLADDGILTGRGSLSVDA
RTNTLVAYQPADRLAELRQLVAQLDVPVRQVAIEARIVEANVDYEKSLGVRWGKPLYAENVRPGKELFVDLGVERAVASV
GLGLLRGDILLDLELSAMEKSGNGEIISQPKVVTADKETARILKGTEVPYQETSKSGATSVSFREASLSLEVTPQITPDN
KVIMTVRVTKDEPDYVNALNNVPPIRKNEVNAKVRVADGETIVIGGVYSTTQNNVVDKVPFFGDLPYVGRLFRRDVLQEK
KSELLVFLTPRIMSDQAIAVSR

Nucleotide


Download         Length: 1269 bp        

>NTDB_id=748403 OGV19_RS25515 WP_264311197.1 5646057..5647325(+) (pilQ) [Pseudomonas putida strain SEH01]
ATGAAGAGGCTATGGAAATGGGTGGCGGCGTTCGCGCTGTCAATCCCGATAGCGTCAGCAACGTCCTACCGGGGTGAGCC
GCTGTCGCTGAACTTTCAGGATGTGCAGGTGCGCGCCGTGTTGCAGGTGCTGGCTGATTACGCCGGTGTGAACCTGGTCG
CCAGCGACTCGGTGCAGGGCAGCATCACCTTGCGCCTGCAGGACGTGCCGTGGGACCAGGCGCTGGATCTGGTGCTGCGC
AGCAAGGGCCTGGCACGCCACGAGGAGGGCAATGTGCTGCTTGTGGCGCCTGCGGCCGAACTCGCCGGGCAATCGCTTGA
CGCCCGGGTGGGCCAGGTGTTGGATGCGCAGTTGCAGCCCTTGCGGCGCGAGCTGATGCCGATCCATCACGCCAAGGCCT
CAGAGCTCGCAGAGCTGTTGCTGGCAACCCTGGCGGACGACGGCATCCTGACCGGGCGAGGCAGTTTGAGCGTCGATGCG
CGCACCAATACCCTGGTGGCGTATCAACCCGCTGATCGCCTTGCCGAGCTGCGCCAACTGGTGGCGCAACTGGATGTGCC
AGTGCGCCAGGTGGCGATCGAGGCGCGCATCGTCGAAGCCAATGTCGATTACGAAAAGAGCCTTGGCGTGCGTTGGGGCA
AGCCCTTGTACGCCGAGAATGTGCGGCCAGGCAAAGAGCTGTTCGTCGACCTTGGGGTGGAACGGGCCGTCGCCAGTGTG
GGCCTGGGCCTGCTGCGGGGAGACATCTTGCTCGACCTTGAGCTCAGTGCCATGGAAAAGAGCGGCAATGGTGAAATCAT
CTCGCAGCCCAAGGTCGTCACGGCCGACAAGGAAACCGCCAGAATCCTCAAAGGCACCGAGGTGCCCTACCAGGAAACCA
GCAAGAGTGGCGCCACCTCCGTGTCGTTTCGCGAAGCGTCGCTGTCGCTGGAGGTCACCCCGCAAATCACCCCGGACAAC
AAGGTCATCATGACCGTCAGGGTGACCAAGGACGAACCGGATTACGTCAATGCCTTGAACAATGTGCCGCCAATCCGCAA
GAACGAGGTAAACGCCAAGGTTCGCGTGGCTGACGGCGAGACGATCGTGATCGGTGGCGTCTACTCGACCACGCAAAACA
ATGTGGTCGACAAGGTGCCATTTTTCGGCGATCTGCCGTATGTTGGGCGGCTGTTTCGACGCGATGTACTACAAGAGAAA
AAATCCGAGCTGCTGGTCTTCCTGACTCCGCGTATCATGAGTGACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(51-97)

Bacterial type II and III secretion system protein (Secretin)

(257-413)

Bacterial type II/III secretion system short domain (Secretin_N)

(125-190)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

58.81

100

0.609
  pilQ Acinetobacter baumannii strain A118

41.739

100

0.455
  pilQ Acinetobacter baumannii D1279779

41.739

100

0.455
  comQ Acinetobacter baylyi ADP1

41.15

100

0.441
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

42.28

99.763

0.422
  pilQ Vibrio cholerae strain A1552

42.28

99.763

0.422
  pilQ Legionella pneumophila strain ERS1305867

42.344

99.052

0.419
  pilQ Legionella pneumophila strain Lp02

42.344

99.052

0.419
  pilQ Vibrio campbellii strain DS40M4

39.636

100

0.412