Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACRAD_RS01365 Genome accession   NZ_AP019740
Coordinates   293344..295053 (-) Length   569 a.a.
NCBI ID   WP_005023073.1    Uniprot ID   A0A062BXA9
Organism   Acinetobacter radioresistens DSM 6976 = NBRC 102413 = CIP 103788 strain NBRC 102413     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 288344..300053
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACRAD_RS01335 (ACRAD_02520) - 288479..288802 (+) 324 WP_005016998.1 pyrimidine/purine nucleoside phosphorylase -
  ACRAD_RS01340 (ACRAD_02530) rlmB 288906..289658 (+) 753 WP_005023068.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  ACRAD_RS01345 (ACRAD_02540) - 289683..290618 (+) 936 WP_005016993.1 DMT family transporter -
  ACRAD_RS01350 (ACRAD_02550) coaE 290615..291226 (-) 612 WP_005016991.1 dephospho-CoA kinase -
  ACRAD_RS01355 (ACRAD_02560) pilD 291228..292088 (-) 861 WP_005016989.1 A24 family peptidase Machinery gene
  ACRAD_RS01360 (ACRAD_02570) pilC 292088..293314 (-) 1227 WP_005016988.1 type II secretion system F family protein Machinery gene
  ACRAD_RS01365 (ACRAD_02580) pilB 293344..295053 (-) 1710 WP_005023073.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACRAD_RS01370 (ACRAD_02590) tpiA 295342..296136 (+) 795 WP_005023075.1 triose-phosphate isomerase -
  ACRAD_RS01375 (ACRAD_02600) secG 296147..296476 (+) 330 WP_005023077.1 preprotein translocase subunit SecG -
  ACRAD_RS01395 (ACRAD_02610) rimP 297103..297627 (+) 525 WP_005016981.1 ribosome maturation factor RimP -
  ACRAD_RS01400 (ACRAD_02620) nusA 297668..299152 (+) 1485 WP_005023080.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 63625.89 Da        Isoelectric Point: 5.0245

>NTDB_id=73736 ACRAD_RS01365 WP_005023073.1 293344..295053(-) (pilB) [Acinetobacter radioresistens DSM 6976 = NBRC 102413 = CIP 103788 strain NBRC 102413]
MTVNFTPKFSGFIRRLVEDNHVSAAEMQNAVDTAKRNRQDIVPYLIDHFKLSPLLIAEFISIEFGEALFDLAVYDTVQIP
RDVVDEKLILKHRVLPLIKRGHTLFVATSNPTNIEALEAIRFNSKLNIEVVIVEHDKLQRTIEQNFSEESNFDFGDEELN
IDVSTSNLDEDDSQDNAPQEEEAPIVKYINKLLVDAIRIGASDLHFEPYEKYYRVRYRVDGVLRQVATPPLQLATRLASR
LKVMSQMDISEKRVPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEPEQKALFIEALEKPQ
GMLLITGPTGSGKTVSLYTGLNILNREDTNISTAEDPVEINLEGINQVNVNNKVGLSFSAALKSFLRQDPDIIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVAAFNIATSVNLVIAQRLARRLCNQCKVPAEIPRNSLLEMGFT
EDDLANPDFQVYQPVGCPECRDGYRGRVGIYEVMKVTPEISKIIMEDGNALEIAATAARLGFDNLRRSGLKKVMQGVTSL
QEINRVTSE

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=73736 ACRAD_RS01365 WP_005023073.1 293344..295053(-) (pilB) [Acinetobacter radioresistens DSM 6976 = NBRC 102413 = CIP 103788 strain NBRC 102413]
ATGACAGTTAATTTTACTCCAAAGTTTTCCGGTTTTATTCGCCGTCTGGTTGAGGATAACCACGTGTCTGCTGCTGAAAT
GCAGAATGCGGTCGATACTGCTAAAAGAAACCGGCAGGATATCGTGCCTTATCTGATCGATCATTTTAAGCTTTCACCCT
TACTGATTGCAGAATTTATCTCGATTGAATTTGGTGAAGCTTTATTCGATTTGGCTGTTTACGATACTGTCCAGATTCCA
CGAGATGTAGTTGATGAGAAACTGATACTCAAGCATCGGGTCTTGCCCCTCATTAAGCGTGGTCATACTCTGTTTGTTGC
TACCAGTAATCCCACCAATATTGAAGCTTTAGAAGCTATCCGGTTTAACAGTAAACTGAATATTGAAGTTGTTATTGTCG
AGCATGACAAACTGCAGCGTACTATTGAGCAGAATTTTTCTGAAGAAAGCAATTTTGATTTTGGTGATGAAGAACTCAAT
ATTGATGTTTCAACCAGCAATTTAGATGAGGACGACAGTCAAGATAATGCGCCTCAGGAAGAAGAAGCTCCAATTGTTAA
ATATATTAACAAGTTATTGGTAGATGCCATTCGTATTGGTGCATCAGACTTACATTTCGAGCCTTATGAAAAATATTACC
GAGTACGTTACCGGGTCGATGGCGTGTTAAGACAGGTGGCAACACCACCTTTACAGCTGGCCACCCGACTGGCTTCACGT
TTAAAGGTTATGTCACAGATGGATATTTCCGAAAAGCGCGTTCCCCAAGATGGCCGGATCAAATTAAAACTCTCTAAGAC
CAAAGCTATTGATTTCCGTGTGAACTCACTGCCTACACTATTTGGAGAAAAACTGGTTCTGCGTATTTTGGATCCGTCCA
GCGCCATGCTGGGTATTGATGCATTAGGCTATGAACCCGAGCAAAAAGCCCTGTTTATAGAAGCTTTAGAAAAGCCGCAA
GGTATGTTACTCATTACCGGGCCTACCGGTTCCGGTAAAACCGTATCGCTTTATACAGGCCTCAATATTTTAAACCGTGA
GGATACCAATATTTCAACTGCCGAAGACCCGGTAGAAATTAATCTTGAAGGTATTAACCAGGTCAATGTGAATAATAAAG
TCGGGTTAAGCTTCTCTGCCGCGCTAAAGTCATTCCTGCGTCAGGATCCAGATATTATTATGGTAGGTGAGATTCGTGAC
TTGGAAACAGCAGAGATTGCCATTAAAGCAGCTCAGACCGGTCACATGGTTATGTCTACCCTGCATACCAACAGCGCTCC
TGAAACATTAACACGCCTAAGAAATATGGGCGTAGCAGCATTTAATATTGCCACTTCAGTTAATCTGGTCATTGCACAGC
GTCTAGCTCGACGTTTATGCAACCAGTGCAAGGTACCAGCAGAGATTCCTAGAAATAGTCTGCTTGAGATGGGCTTTACC
GAAGATGACTTGGCCAACCCTGATTTTCAGGTTTATCAACCTGTCGGTTGCCCGGAATGTCGTGATGGTTATCGGGGTCG
TGTGGGTATCTATGAAGTAATGAAAGTTACACCGGAAATTTCAAAAATTATTATGGAAGACGGTAACGCCCTTGAAATAG
CAGCAACAGCAGCACGCCTGGGTTTTGATAACTTGCGTCGTTCTGGCCTAAAGAAAGTCATGCAAGGTGTAACCTCTTTA
CAAGAAATTAACCGTGTGACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(56-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A062BXA9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

81.383

99.121

0.807
  pilB Acinetobacter baylyi ADP1

78.33

98.946

0.775
  pilB Legionella pneumophila strain ERS1305867

53.676

95.606

0.513
  pilF Neisseria gonorrhoeae MS11

49.644

98.77

0.49
  pilB Vibrio parahaemolyticus RIMD 2210633

51.718

92.091

0.476
  pilB Vibrio cholerae strain A1552

50.478

91.916

0.464
  pilB Vibrio campbellii strain DS40M4

50.287

91.916

0.462
  pilB/pilB1 Synechocystis sp. PCC 6803

34.516

100

0.376
  pilF Thermus thermophilus HB27

36.525

99.121

0.362

Multiple sequence alignment