Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NBH81_RS02265 Genome accession   NZ_CP106828
Coordinates   459125..460831 (-) Length   568 a.a.
NCBI ID   WP_005336134.1    Uniprot ID   -
Organism   Aeromonas veronii strain jsyz553301     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 454125..465831
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NBH81_RS02235 (NBH81_02235) mutT 454636..455040 (+) 405 WP_019444787.1 8-oxo-dGTP diphosphatase MutT -
  NBH81_RS02240 (NBH81_02240) yacG 455118..455312 (-) 195 WP_005336142.1 DNA gyrase inhibitor YacG -
  NBH81_RS02245 (NBH81_02245) zapD 455322..456044 (-) 723 WP_005336141.1 cell division protein ZapD -
  NBH81_RS02250 (NBH81_02250) coaE 456082..456696 (-) 615 WP_201938611.1 dephospho-CoA kinase -
  NBH81_RS02255 (NBH81_02255) pilD 456709..457581 (-) 873 WP_263085266.1 A24 family peptidase Machinery gene
  NBH81_RS02260 (NBH81_02260) pilC 457643..458884 (-) 1242 WP_005336136.1 type II secretion system F family protein Machinery gene
  NBH81_RS02265 (NBH81_02265) pilB 459125..460831 (-) 1707 WP_005336134.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  NBH81_RS02270 (NBH81_02270) - 460835..461278 (-) 444 WP_263085267.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  NBH81_RS02275 (NBH81_02275) nadC 461594..462457 (-) 864 WP_263085268.1 carboxylating nicotinate-nucleotide diphosphorylase -
  NBH81_RS02280 (NBH81_02280) - 462461..462931 (-) 471 WP_040068932.1 TIGR02281 family clan AA aspartic protease -
  NBH81_RS02285 (NBH81_02285) ampD 463059..463634 (+) 576 WP_263085269.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NBH81_RS02290 (NBH81_02290) pdhR 463913..464677 (+) 765 WP_100652550.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62533.87 Da        Isoelectric Point: 5.3192

>NTDB_id=735942 NBH81_RS02265 WP_005336134.1 459125..460831(-) (pilB) [Aeromonas veronii strain jsyz553301]
MTSSPNSGLALSLAASSLISEEDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTAAIGKLLESENDALGMEDIDESEI
SDLEVSDENSRLDESVNTADDDAPIVKYINKILMDAIKRGASDLHFEPYEFKYRVRFRIDGILHEIATPPVNLSNRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
PQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EVNRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=735942 NBH81_RS02265 WP_005336134.1 459125..460831(-) (pilB) [Aeromonas veronii strain jsyz553301]
ATGACCTCTAGCCCTAATAGCGGCCTGGCGTTAAGCCTGGCCGCCTCTTCATTGATCAGTGAAGAGGACTCCCAGCGCTA
TCTGAGCCAGGCCAAGGCCCAGCGCAAACCCTTTGTGACATTTCTGATAGAGAACGAGATCCTCGACAGCAAGGCGCTGG
CCGACTTTTGTGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGATTTGGCCGAGATCCCGCAGAAGTAC
CTCAATCAGAAGCTGATTGAGAAACACCATGTGCTGCCCATCTACACCCAGGGCCACACCCTCTATATCGCCATGTCGGA
TCCGACCAATGTCTCGGCGTTGGAGGACTTTGGCTTCAGCTTCGGGCTACATACCGAAGCGTTGCTGGTGGAGGAGAACA
AACTCACCGCCGCCATCGGCAAGCTGCTGGAGAGCGAAAATGACGCCCTCGGTATGGAGGATATCGACGAGTCCGAGATT
TCGGATCTCGAGGTCTCCGACGAGAACAGCCGGCTGGACGAGAGCGTCAATACTGCCGATGACGATGCTCCCATCGTCAA
GTACATCAACAAGATCCTGATGGACGCCATCAAGCGCGGAGCCTCCGACCTGCACTTCGAGCCATATGAGTTCAAGTATC
GGGTGCGCTTTCGCATCGATGGCATTCTGCATGAAATCGCTACCCCGCCGGTCAACCTCTCCAACCGCTTCTCCGCCCGC
CTCAAGGTGATGGCGCGACTCGATATCGCCGAGCGGCGGCTGCCACAGGATGGCCGCATCAAGCTGAAGCTGTCGCGCAA
CAAGTCGATGGATATGCGGGTCAACACCCTGCCCACCATGTGGGGGGAGAAGATCGTTATCCGTCTGCTCGACTCCTCGG
CGGCACGCCTCAATATCGAGCAACTCGGCTTTGATGACCGGCAGAAGGCGCAGTACCTGCGCGCCCTCTCCAAGCCACAG
GGGATGATCCTGGTGACCGGCCCCACCGGCTCGGGCAAAACGGTCTCGCTCTATACCGGCCTAAATATTCTCAACACCAC
CGAGGTCAATATCTCTACCGCCGAAGATCCGGTGGAGATCAACCTGCCCGGCGTCAATCAGGTGCAGGTCAATCCCAAGG
CGGGGCTCACCTTTGCCAGCGCCCTGCGCTCCTTCTTGCGGCAAGACCCGGATGTGGTGATGGTGGGTGAAATTCGCGAT
CTGGAAACTGCCGAAATCGCCATCAAGGCAGCACAAACCGGCCACCTGGTGCTCTCCACCCTGCACACCAACTCCGCCGC
AGAGACCCTGACCCGGATGATGAACATGGGGGTGCCTGCTTTCAACATCGCCTCCTCGGTAACCCTGATCATGGCCCAGC
GGCTGGCCCGTAAACTATGCGACCACTGCAAAGCGCCGGAAGTGGTACCGGAAGCCGAACTGCTGGAGCTGGGTTTTACC
CCGCAGCAGTTGGCGGCGGGGCTGCGACTGTTCAAACCGGTCGGCTGCAAGGAGTGTTCCGGCGGCTACAAGGGGCGGGT
CGGCATCTACGAAATCATGCTGATGTCAGAGAACATCGCCAAATTGATTATGCAGGGGGCCAACTCCCTGCAAATCGCCG
CTATCGCCCAGAAAGAGGGGATGCGTACCTTACGTACCTCGGGACTAGAGAAGGCCCGGCTCGGGGTCACCAGTCTGGCC
GAGGTCAACCGGGTCACAACCAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.986

100

0.56
  pilB Acinetobacter baylyi ADP1

55.789

100

0.56
  pilB Legionella pneumophila strain ERS1305867

56.642

95.423

0.54
  pilB Vibrio cholerae strain A1552

54.758

98.063

0.537
  pilB Vibrio campbellii strain DS40M4

51.22

100

0.518
  pilB Vibrio parahaemolyticus RIMD 2210633

51.596

99.296

0.512
  pilF Neisseria gonorrhoeae MS11

46.809

99.296

0.465
  pilB/pilB1 Synechocystis sp. PCC 6803

39.06

97.359

0.38
  pilF Thermus thermophilus HB27

40.53

92.958

0.377