Detailed information NTG0734531

Bioinformatically predicted

Overview

• Name: comGA
• Type: Machinery gene
• Locus tag: P4831_RS12060
• Genome accession: NZ_CP120842
• Coordinates: 2311567..2312505 (-)
• Length: 312 a.a.
• NCBI ID: WP_031561106.1
• Uniprot ID: -
• Organism: Lactococcus lactis strain ZFM559
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2306567..2317505

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
P4831_RS12025	-	2306868..2307074 (-)	207	WP_153927607.1	helix-turn-helix transcriptional regulator	-
P4831_RS12030	-	2307234..2307635 (+)	402	WP_153927608.1	helix-turn-helix transcriptional regulator	-
P4831_RS12035	-	2307647..2308246 (+)	600	WP_277812525.1	hypothetical protein	-
P4831_RS12040	-	2308327..2308866 (+)	540	WP_251921194.1	PH domain-containing protein	-
P4831_RS12045	-	2308986..2310449 (+)	1464	WP_277812526.1	recombinase family protein	-
P4831_RS12050	-	2310446..2310586 (-)	141	WP_023164653.1	hypothetical protein	-
P4831_RS12055	comGB	2310600..2311673 (-)	1074	WP_010906319.1	competence type IV pilus assembly protein ComGB	Machinery gene
P4831_RS12060	comGA	2311567..2312505 (-)	939	WP_031561106.1	competence type IV pilus ATPase ComGA	Machinery gene

Sequence

Protein

• Length: 312 a.a.
• Molecular weight: 35707.88 Da
• Isoelectric Point: 5.9473

>NTDB_id=734531 P4831_RS12060 WP_031561106.1 2311567..2312505(-) (comGA) [Lactococcus lactis strain ZFM559]
MIPKMAKELIQRAIDRDASDIYLIASKEKYKLYFRQMTARDLVEEIGLEVGLALLTHFKFLAGMNTGERRRVQLGACWYE
IEESNAKRLRLSTVGDFEGNESLVIRLLHDQNKELDFWFDEELENYDCKRGLHLFAGPVGSGKTSLMFDLARRHFTDLQV
ITIEEPVELIDSDFIQLQVNEVIGNSYDELIKLSLRHRPDLLIVGEIRDKLTAQAVLRASLTGYTVFSTVHASSVRGVIQ
RLSELGLSHWEIKNALSSVVYQRLIAGKGVLDIAKNEFESWSSENWNKKIDQLLTNGYLTPTEAKREKIKTD

Nucleotide

• Length: 939 bp

>NTDB_id=734531 P4831_RS12060 WP_031561106.1 2311567..2312505(-) (comGA) [Lactococcus lactis strain ZFM559]
ATGATACCAAAAATGGCAAAAGAATTAATTCAAAGAGCAATTGATAGAGATGCATCAGATATTTATCTGATTGCTTCAAA
AGAAAAGTATAAACTTTATTTTCGACAAATGACAGCACGTGATCTGGTAGAAGAAATTGGGTTAGAAGTTGGCTTAGCAC
TACTTACTCACTTTAAGTTTCTAGCTGGGATGAATACAGGAGAACGCAGACGAGTTCAATTGGGAGCTTGTTGGTATGAA
ATAGAGGAAAGTAATGCAAAACGTTTGCGTCTTTCGACAGTGGGGGATTTCGAGGGTAATGAATCTTTAGTAATACGTCT
CTTGCATGATCAAAACAAAGAACTTGATTTTTGGTTTGATGAAGAGCTAGAGAATTATGATTGTAAAAGAGGATTGCATC
TCTTTGCCGGTCCAGTAGGCTCGGGAAAAACATCTTTAATGTTTGATTTAGCTCGTCGTCATTTTACAGATTTACAAGTT
ATCACTATTGAAGAACCCGTTGAGTTGATTGATTCCGATTTTATTCAGTTACAAGTCAACGAAGTCATTGGTAATAGTTA
TGATGAATTAATTAAATTATCACTGAGGCATCGCCCAGATTTGTTAATCGTTGGTGAAATTCGTGATAAATTGACCGCTC
AAGCAGTTTTACGGGCTAGTTTGACAGGTTATACAGTGTTTTCAACCGTTCATGCCTCTTCTGTTAGAGGTGTCATTCAG
AGATTATCAGAGCTGGGTTTAAGTCATTGGGAAATAAAAAATGCTTTATCAAGTGTTGTTTATCAGCGTTTAATAGCTGG
GAAAGGAGTATTAGACATTGCCAAAAACGAATTTGAGTCTTGGTCATCAGAAAATTGGAATAAAAAAATTGATCAGCTAC
TTACAAATGGATATCTCACACCTACTGAAGCCAAAAGGGAAAAAATTAAGACTGATTAA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (9-265)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGA	Lactococcus lactis subsp. cremoris KW2	76.923	100	0.769
comGA/cglA	Streptococcus sobrinus strain NIDR 6715-7	54.984	99.679	0.548
comGA/cglA/cilD	Streptococcus mitis NCTC 12261	53.376	99.679	0.532
comGA/cglA/cilD	Streptococcus pneumoniae Rx1	52.412	99.679	0.522
comGA/cglA/cilD	Streptococcus pneumoniae D39	52.412	99.679	0.522
comGA/cglA/cilD	Streptococcus pneumoniae R6	52.412	99.679	0.522
comGA/cglA/cilD	Streptococcus pneumoniae TIGR4	52.412	99.679	0.522
comGA	Streptococcus mutans UA159	52.244	100	0.522
comGA	Streptococcus mutans UA140	52.244	100	0.522
comGA	Streptococcus gordonii str. Challis substr. CH1	51.923	100	0.519