Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   N8A97_RS28600 Genome accession   NZ_CP104901
Coordinates   5945480..5946700 (+) Length   406 a.a.
NCBI ID   WP_009315898.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain DB-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5940480..5951700
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N8A97_RS28580 (N8A97_28575) nadC 5942067..5942915 (+) 849 WP_003161760.1 carboxylating nicotinate-nucleotide diphosphorylase -
  N8A97_RS28590 (N8A97_28585) pilA 5943092..5943544 (-) 453 WP_024928732.1 pilin Machinery gene
  N8A97_RS28595 (N8A97_28590) pilB 5943776..5945476 (+) 1701 WP_010793793.1 type IV-A pilus assembly ATPase PilB Machinery gene
  N8A97_RS28600 (N8A97_28595) pilC 5945480..5946700 (+) 1221 WP_009315898.1 type 4a pilus biogenesis protein PilC Machinery gene
  N8A97_RS28605 (N8A97_28600) pilD 5946704..5947576 (+) 873 WP_003125204.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  N8A97_RS28610 (N8A97_28605) coaE 5947573..5948184 (+) 612 WP_010793792.1 dephospho-CoA kinase -
  N8A97_RS28615 (N8A97_28610) yacG 5948181..5948381 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  N8A97_RS28620 (N8A97_28615) - 5948418..5948627 (-) 210 WP_003094660.1 hypothetical protein -
  N8A97_RS28625 (N8A97_28620) - 5948733..5949422 (-) 690 WP_003094662.1 energy-coupling factor ABC transporter permease -
  N8A97_RS28630 (N8A97_28625) - 5949419..5949889 (-) 471 WP_010793791.1 hypothetical protein -
  N8A97_RS28635 (N8A97_28630) - 5949886..5950311 (-) 426 WP_003103865.1 GNAT family N-acetyltransferase -
  N8A97_RS28640 (N8A97_28635) - 5950445..5951074 (+) 630 WP_003094668.1 DUF1780 domain-containing protein -
  N8A97_RS28645 (N8A97_28640) - 5951071..5951520 (+) 450 WP_003094670.1 MOSC domain-containing protein -

Sequence

Protein


Download         Length: 406 a.a.        Molecular weight: 44288.04 Da        Isoelectric Point: 9.8784

>NTDB_id=733272 N8A97_RS28600 WP_009315898.1 5945480..5946700(+) (pilC) [Pseudomonas aeruginosa strain DB-1]
MADKALKTSVFIWEGTDKKGAKVKGELTGQNPMLVKAHLRKQGINPLKVRKKGISLLGAGKKVKPMDIALFTRQMATMMG
AGVPLLQSFDIIGEGFDNPNMRKLVDEIKQEVSSGNSLANSLRKKPQYFDELYCNLVDAGEQSGALENLLDRVATYKEKT
ESLKAKIRKAMTYPIAVIIVALIVSAILLIKVVPQFQSVFQGFGAELPAFTQMVVNLSEFLQEWWLAVIVGVGAIGFTFK
ELHKRSKKFRDALDRTILKLPIFGGIVYKSAVARYARTLSTTFAAGVPLVDALDSVSGATGNIVFKNAVSKIKQDVSTGM
QLNFSMRTTSVFPNMAIQMTAIGEESGSLDEMLSKVASYYEEEVDNAVDNLTTLMEPMIMAVLGVLVGGLIVAMYLPIFQ
LGNVVG

Nucleotide


Download         Length: 1221 bp        

>NTDB_id=733272 N8A97_RS28600 WP_009315898.1 5945480..5946700(+) (pilC) [Pseudomonas aeruginosa strain DB-1]
ATGGCGGACAAAGCGTTAAAAACCAGCGTTTTCATCTGGGAGGGCACCGACAAGAAAGGCGCCAAGGTCAAGGGCGAACT
GACCGGGCAGAATCCCATGCTGGTGAAGGCCCATCTGCGCAAGCAAGGCATCAATCCGCTCAAGGTACGCAAGAAAGGTA
TCTCCCTGCTGGGCGCAGGCAAGAAAGTCAAACCCATGGACATCGCCCTGTTCACCCGGCAGATGGCGACCATGATGGGC
GCTGGCGTTCCCCTCCTGCAATCGTTCGACATCATCGGCGAAGGCTTCGACAATCCCAACATGCGCAAGTTGGTGGATGA
GATCAAGCAGGAAGTGTCCTCGGGCAACAGCCTGGCCAACTCACTGAGAAAGAAACCACAGTACTTCGACGAACTCTATT
GCAACCTGGTCGATGCCGGCGAGCAATCGGGCGCCTTGGAAAACTTGCTGGATAGAGTCGCGACCTACAAGGAAAAAACG
GAATCGTTAAAAGCAAAGATCAGGAAGGCGATGACTTATCCTATCGCCGTCATCATCGTTGCCTTGATCGTCTCCGCCAT
TCTTCTGATCAAGGTCGTTCCGCAGTTCCAATCCGTATTCCAAGGTTTTGGCGCAGAGCTGCCTGCATTCACTCAAATGG
TCGTAAATTTATCCGAGTTCCTACAGGAGTGGTGGCTTGCCGTCATCGTTGGTGTGGGGGCGATTGGCTTTACCTTCAAG
GAACTGCATAAACGTTCGAAAAAATTCCGCGACGCACTCGACAGAACGATCCTCAAACTTCCCATTTTCGGAGGCATCGT
CTACAAATCCGCGGTCGCCCGTTATGCACGGACCTTGTCCACGACCTTCGCCGCAGGTGTTCCCCTGGTCGATGCGCTCG
ACTCCGTCTCCGGAGCGACCGGCAATATCGTGTTCAAGAACGCGGTCAGCAAGATCAAGCAAGACGTTTCCACCGGCATG
CAGCTCAACTTCTCCATGCGCACCACCAGCGTCTTTCCCAACATGGCGATCCAGATGACCGCCATCGGCGAGGAGTCCGG
TTCGCTCGATGAGATGCTGAGCAAAGTCGCCAGCTACTACGAAGAGGAAGTCGACAACGCCGTGGACAACCTCACCACGC
TCATGGAACCGATGATCATGGCCGTTCTCGGCGTACTGGTTGGCGGTCTGATCGTGGCCATGTACCTTCCGATCTTCCAA
CTCGGCAACGTCGTCGGATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

77.037

99.754

0.768
  pilC Acinetobacter baumannii D1279779

60.891

99.507

0.606
  pilC Acinetobacter baylyi ADP1

60.591

100

0.606
  pilC Legionella pneumophila strain ERS1305867

54.293

97.537

0.53
  pilG Neisseria meningitidis 44/76-A

45.658

99.261

0.453
  pilG Neisseria gonorrhoeae MS11

45.409

99.261

0.451
  pilC Vibrio cholerae strain A1552

42.611

100

0.426
  pilC Vibrio campbellii strain DS40M4

41.562

97.783

0.406
  pilC Thermus thermophilus HB27

37.157

98.768

0.367