Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   GZK95_RS01145 Genome accession   NZ_AP019654
Coordinates   249343..251013 (+) Length   556 a.a.
NCBI ID   WP_075716334.1    Uniprot ID   -
Organism   Vibrio panuliri strain JCM 19500     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 244343..256013
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GZK95_RS01125 pilM 246750..247568 (+) 819 WP_161987190.1 type IV pilus assembly protein PilM -
  GZK95_RS01130 - 247698..248270 (+) 573 WP_075716301.1 PilN domain-containing protein -
  GZK95_RS01135 - 248260..248853 (+) 594 WP_075716302.1 type 4a pilus biogenesis protein PilO -
  GZK95_RS01140 - 248843..249316 (+) 474 WP_075716303.1 pilus assembly protein PilP -
  GZK95_RS01145 pilQ 249343..251013 (+) 1671 WP_075716334.1 type IV pilus secretin PilQ Machinery gene
  GZK95_RS01150 aroK 251178..251696 (+) 519 WP_075711226.1 shikimate kinase AroK -
  GZK95_RS01155 aroB 251727..252830 (+) 1104 WP_075716304.1 3-dehydroquinate synthase -
  GZK95_RS01160 - 252852..254306 (+) 1455 WP_075716305.1 SPOR domain-containing protein -
  GZK95_RS01165 - 254462..255286 (+) 825 WP_075716306.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -

Sequence

Protein


Download         Length: 556 a.a.        Molecular weight: 61315.86 Da        Isoelectric Point: 4.9104

>NTDB_id=73074 GZK95_RS01145 WP_075716334.1 249343..251013(+) (pilQ) [Vibrio panuliri strain JCM 19500]
MFMFTISLPVSGEEPPRINRFVGVDFQLDSEKNGNIIIEMSEQQIALDISKTEQGLTLDLPKTELMPHQAKRFELLDFAT
NVQAIELFEHGSRVRIKVQISGEFDYQYHIENKQIELIVWPLKADPQTPHVLAAKKKISINFQDVPVRNVLQLIADYNGF
NLVASDSVKGNLSLRLDDVPWTDVLEVILQVKGLDKRIHGNVVLVAPLAEIELREQQRFERSRIERERVGFQSQILHINF
ANARDIAELFSGEGAMGVLSEGGSVRVDERTNSLLVRDFATNIEAISRIVASLDIPVKQVQIEARIVTVSEGSLDELGVR
WSLNSYHGDLGGSLEGNFAQRQQANMLPSGKGFGPNDDISDFLNVNLASSASNASTIAFQVAKLGSHTLLDLELSALQRE
SKAEVISSPRLITTNKQPAYIEQGTEIPYLEAASSGATSVSFKKAVLSLKVTPQITPDDRLILDLSVTQDRPGEVLKTGT
GEAVSINTQRIGTQVLVEDGETIVLGGIYQHSVTDTVDKVPLLGDIPLLGALFRRSYQQLGKSELLIFVTPKVVVH

Nucleotide


Download         Length: 1671 bp        

>NTDB_id=73074 GZK95_RS01145 WP_075716334.1 249343..251013(+) (pilQ) [Vibrio panuliri strain JCM 19500]
ATGTTTATGTTCACAATCAGCTTGCCTGTATCAGGGGAAGAACCACCGCGAATCAACCGTTTTGTTGGGGTCGATTTCCA
ATTGGATAGTGAAAAAAACGGCAACATCATTATCGAAATGAGTGAGCAACAGATTGCTCTAGATATCAGTAAGACAGAGC
AAGGTTTGACTCTAGATTTACCTAAAACTGAACTGATGCCGCATCAGGCTAAGCGTTTTGAACTGCTCGATTTTGCCACG
AATGTTCAAGCGATAGAACTGTTTGAGCATGGGAGTCGTGTGCGGATTAAAGTGCAGATCAGTGGTGAGTTTGACTATCA
GTACCATATTGAGAATAAACAAATTGAGTTGATAGTGTGGCCGTTAAAAGCAGACCCACAAACACCTCACGTGTTGGCCG
CTAAGAAAAAGATATCGATTAATTTTCAGGATGTGCCGGTGCGGAATGTCTTACAGTTAATTGCCGATTACAACGGCTTC
AATTTGGTCGCTTCAGACTCGGTTAAGGGCAACCTTTCGCTGCGTTTAGATGATGTTCCTTGGACAGATGTATTAGAGGT
AATTTTGCAGGTAAAGGGCTTGGACAAACGGATCCATGGCAATGTCGTATTGGTGGCCCCGTTAGCGGAAATTGAGCTTA
GAGAGCAGCAGCGTTTTGAGCGCTCGCGTATCGAAAGGGAGCGAGTGGGCTTTCAATCGCAAATTCTGCATATCAACTTT
GCTAATGCGCGTGACATCGCGGAACTGTTTAGTGGTGAAGGCGCAATGGGCGTGCTATCTGAAGGAGGTTCTGTTCGCGT
CGATGAGCGTACCAACTCTTTGCTGGTGAGGGATTTTGCCACCAATATTGAAGCGATTAGTCGAATTGTTGCTTCGTTAG
ATATTCCGGTCAAACAGGTGCAGATTGAAGCGCGGATTGTGACGGTCAGTGAAGGGAGTCTGGATGAGCTAGGCGTGCGC
TGGAGTCTTAACTCTTATCACGGTGACTTGGGTGGCTCGTTAGAGGGGAACTTTGCTCAACGTCAGCAAGCTAATATGCT
GCCATCGGGCAAGGGCTTTGGGCCAAACGATGATATTAGTGATTTTCTTAATGTGAATCTCGCTTCGTCTGCGTCGAACG
CATCGACAATCGCCTTTCAAGTGGCTAAGTTAGGCAGCCATACATTGCTCGATTTAGAACTATCGGCATTGCAACGTGAA
TCAAAGGCCGAAGTGATTTCGAGTCCTCGTTTAATCACAACCAATAAGCAGCCAGCCTATATCGAACAAGGTACTGAAAT
TCCATATTTGGAAGCTGCGTCGAGTGGTGCGACATCAGTTTCCTTTAAAAAAGCGGTATTAAGCCTTAAAGTAACGCCGC
AAATTACCCCAGATGACCGTCTAATCCTGGATTTAAGTGTTACTCAAGATAGACCTGGCGAAGTGTTAAAGACCGGTACT
GGAGAAGCAGTGTCGATTAATACTCAACGCATTGGTACGCAAGTGCTGGTGGAGGATGGCGAAACCATCGTTTTGGGGGG
AATATATCAACATAGTGTGACCGATACGGTAGATAAAGTGCCGTTACTCGGGGACATTCCACTGCTTGGTGCATTATTTA
GGCGCAGCTACCAGCAATTAGGCAAAAGTGAGCTGCTGATTTTCGTTACGCCCAAAGTAGTTGTGCATTAA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(396-554)

Bacterial type II/III secretion system short domain (Secretin_N)

(234-299)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae strain A1552

60.364

98.921

0.597
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

60.364

98.921

0.597
  pilQ Vibrio campbellii strain DS40M4

59.855

99.46

0.595
  pilQ Legionella pneumophila strain ERS1305867

39.815

97.122

0.387
  pilQ Legionella pneumophila strain Lp02

39.815

97.122

0.387
  pilQ Pseudomonas aeruginosa PAK

38.391

98.381

0.378
  pilQ Acinetobacter baumannii strain A118

34.848

100

0.372
  pilQ Acinetobacter baumannii D1279779

34.848

100

0.372
  comQ Acinetobacter baylyi ADP1

35.223

100

0.369

Multiple sequence alignment