Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   N5E84_RS10360 Genome accession   NZ_CP104554
Coordinates   2264625..2266313 (+) Length   562 a.a.
NCBI ID   WP_029388146.1    Uniprot ID   A0A289G7S6
Organism   Vibrio sp. J502     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2259625..2271313
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N5E84_RS10330 (N5E84_10330) - 2259978..2260751 (-) 774 WP_017045785.1 thioredoxin fold domain-containing protein -
  N5E84_RS10335 (N5E84_10335) xerD 2260765..2261673 (-) 909 WP_013857679.1 site-specific tyrosine recombinase XerD -
  N5E84_RS10340 (N5E84_10340) fldB 2261826..2262344 (+) 519 WP_013857680.1 flavodoxin FldB -
  N5E84_RS10345 (N5E84_10345) ampD 2262366..2262917 (-) 552 WP_214650766.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  N5E84_RS10350 (N5E84_10350) nadC 2263042..2263929 (+) 888 WP_019281900.1 carboxylating nicotinate-nucleotide diphosphorylase -
  N5E84_RS10355 (N5E84_10355) - 2264185..2264619 (+) 435 WP_050934217.1 type IV pilin protein -
  N5E84_RS10360 (N5E84_10360) pilB 2264625..2266313 (+) 1689 WP_029388146.1 type IV-A pilus assembly ATPase PilB Machinery gene
  N5E84_RS10365 (N5E84_10365) pilC 2266356..2267582 (+) 1227 WP_069212069.1 type II secretion system F family protein Machinery gene
  N5E84_RS10370 (N5E84_10370) pilD 2267621..2268502 (+) 882 WP_019281902.1 prepilin peptidase Machinery gene
  N5E84_RS10375 (N5E84_10375) coaE 2268493..2269101 (+) 609 WP_013857688.1 dephospho-CoA kinase -
  N5E84_RS10380 (N5E84_10380) zapD 2269134..2269874 (+) 741 WP_019281903.1 cell division protein ZapD -
  N5E84_RS10385 (N5E84_10385) yacG 2269964..2270161 (+) 198 WP_010319526.1 DNA gyrase inhibitor YacG -
  N5E84_RS10390 (N5E84_10390) rplS 2270231..2270584 (-) 354 WP_010319525.1 50S ribosomal protein L19 -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62291.43 Da        Isoelectric Point: 6.4458

>NTDB_id=730438 N5E84_RS10360 WP_029388146.1 2264625..2266313(+) (pilB) [Vibrio sp. J502]
MLTNLPAILRQAQILSPTQEHAVIEHIKASGANVPEALLALDFFHPHDLTEQLSTLFGLPDTDLTRYDYPQLCQQLGLRD
LITRYQALPIAHNGQTLTIAVSDPTHLQVEDDFRFATGLQVELVLADYKALQAAIRRLYGRTIQGSSSSGKEISQDELAN
LVKLSDDEIQSIEDLSQDDAPVSRFINQILLDAVRKGASDIHFEPYEALYRVRLRCDGILVETQRPSSNLSRRLAARLKI
LSKLDIAERRLPQDGRIKLRLNQDTAIDMRVSSLPTLWGEKIVLRLLDSSAANLDIDKLGYNDQQKQLYLAALKKPQGMI
LMTGPTGSGKTVSLYTGLRILNTAEVNISTAEDPVEINLSGINQVQVQPKIGFGFAQALRSFLRQDPDIVMVGEIRDLET
AEIAVKAAQTGHLVLSTLHTNSAAETVIRLSNMGIEAFNLASSLSLIIAQRLARRLCRHCKIAVVPSMQLQQHFQLLATE
TIYEANPHGCNECTGGYSGRVGIYEVMAFNPQLAEAIMQKASIHQIEQIAKANGMQTLQESGIEKLKNGVTSFKELQRVL
YF

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=730438 N5E84_RS10360 WP_029388146.1 2264625..2266313(+) (pilB) [Vibrio sp. J502]
ATGCTAACCAACCTCCCTGCGATTCTGCGTCAGGCTCAGATATTGAGCCCGACGCAAGAACACGCAGTTATTGAACATAT
CAAAGCGTCTGGTGCGAATGTGCCAGAAGCTTTGCTCGCTTTGGATTTTTTCCATCCTCATGATCTGACTGAGCAGTTAA
GTACCCTTTTTGGCCTACCGGACACCGACTTAACCCGCTACGACTACCCACAGCTCTGCCAACAGCTTGGTTTGCGTGAT
CTCATCACCCGTTACCAAGCACTGCCGATTGCCCATAATGGCCAAACCCTGACCATCGCGGTTTCCGACCCGACCCATTT
ACAAGTGGAAGATGATTTTCGCTTCGCGACAGGCTTACAAGTTGAACTGGTTTTAGCCGACTACAAAGCGCTGCAAGCAG
CCATCCGGCGCTTGTATGGCCGAACGATTCAAGGATCCTCTTCTTCAGGGAAAGAGATCAGCCAAGATGAACTGGCGAAC
TTAGTTAAGCTATCTGATGATGAGATTCAATCGATTGAAGACCTCAGCCAAGATGACGCCCCAGTCAGCCGTTTTATTAA
CCAAATCCTGCTTGATGCGGTGCGAAAAGGCGCATCCGATATCCATTTCGAACCGTACGAAGCGCTTTACCGAGTACGAT
TGCGCTGTGATGGTATTTTGGTGGAAACGCAGCGTCCCTCAAGCAACTTAAGTCGCCGGCTTGCCGCGCGGCTTAAAATT
TTATCCAAACTCGATATTGCCGAGCGGCGCTTACCACAAGATGGACGAATTAAACTGCGGTTGAATCAAGACACCGCCAT
CGATATGCGCGTTTCCAGCCTCCCCACTTTATGGGGTGAAAAAATCGTGCTACGTCTACTCGATAGCAGCGCTGCTAACT
TAGATATCGACAAATTGGGTTATAACGACCAGCAAAAACAGCTCTACTTAGCTGCGCTGAAAAAACCACAAGGGATGATT
TTAATGACGGGCCCGACGGGCAGCGGAAAAACCGTCTCGCTCTATACGGGGCTACGCATTTTAAATACTGCTGAAGTGAA
CATTTCCACCGCTGAAGATCCGGTTGAAATTAACTTATCCGGTATCAACCAAGTTCAAGTGCAACCAAAAATTGGCTTTG
GCTTTGCGCAAGCATTACGCTCCTTCTTACGGCAAGATCCCGACATTGTGATGGTTGGCGAAATCCGCGATTTAGAAACT
GCCGAAATCGCCGTGAAAGCGGCACAAACCGGGCATTTAGTTCTTTCTACCTTGCATACTAACTCCGCCGCCGAAACGGT
AATTCGCTTGAGTAATATGGGAATTGAAGCGTTTAACTTAGCCTCTTCACTCAGTTTAATTATTGCCCAGCGTTTGGCTC
GTCGCCTTTGTCGGCACTGCAAAATTGCCGTCGTGCCCTCAATGCAGTTGCAGCAACATTTTCAGCTGCTAGCGACAGAA
ACCATTTATGAAGCGAACCCGCATGGTTGCAACGAATGCACAGGGGGCTATTCAGGTCGAGTCGGCATTTATGAAGTGAT
GGCGTTTAACCCACAGCTGGCCGAAGCGATTATGCAAAAAGCAAGCATTCATCAAATAGAACAGATCGCGAAAGCCAACG
GAATGCAGACACTACAAGAATCAGGCATTGAAAAGCTCAAAAACGGCGTCACCAGCTTCAAAGAGCTGCAACGCGTGCTC
TATTTCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-141)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A289G7S6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

83.096

100

0.831
  pilB Vibrio campbellii strain DS40M4

74.377

100

0.744
  pilB Vibrio parahaemolyticus RIMD 2210633

72.954

100

0.73
  pilB Acinetobacter baumannii D1279779

52.115

92.527

0.482
  pilB Legionella pneumophila strain ERS1305867

51.758

91.103

0.472
  pilB Acinetobacter baylyi ADP1

50.38

93.594

0.472
  pilF Neisseria gonorrhoeae MS11

44.248

100

0.445
  pilF Thermus thermophilus HB27

37.589

100

0.377
  pilB/pilB1 Synechocystis sp. PCC 6803

35.763

100

0.375