Detailed information NTG0730440

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: N5E84_RS10370
• Genome accession: NZ_CP104554
• Coordinates: 2267621..2268502 (+)
• Length: 293 a.a.
• NCBI ID: WP_019281902.1
• Uniprot ID: A0A289G9B4
• Organism: Vibrio sp. J502
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2262621..2273502

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
N5E84_RS10350 (N5E84_10350)	nadC	2263042..2263929 (+)	888	WP_019281900.1	carboxylating nicotinate-nucleotide diphosphorylase	-
N5E84_RS10355 (N5E84_10355)	-	2264185..2264619 (+)	435	WP_050934217.1	type IV pilin protein	-
N5E84_RS10360 (N5E84_10360)	pilB	2264625..2266313 (+)	1689	WP_029388146.1	type IV-A pilus assembly ATPase PilB	Machinery gene
N5E84_RS10365 (N5E84_10365)	pilC	2266356..2267582 (+)	1227	WP_069212069.1	type II secretion system F family protein	Machinery gene
N5E84_RS10370 (N5E84_10370)	pilD	2267621..2268502 (+)	882	WP_019281902.1	prepilin peptidase	Machinery gene
N5E84_RS10375 (N5E84_10375)	coaE	2268493..2269101 (+)	609	WP_013857688.1	dephospho-CoA kinase	-
N5E84_RS10380 (N5E84_10380)	zapD	2269134..2269874 (+)	741	WP_019281903.1	cell division protein ZapD	-
N5E84_RS10385 (N5E84_10385)	yacG	2269964..2270161 (+)	198	WP_010319526.1	DNA gyrase inhibitor YacG	-
N5E84_RS10390 (N5E84_10390)	rplS	2270231..2270584 (-)	354	WP_010319525.1	50S ribosomal protein L19	-
N5E84_RS10395 (N5E84_10395)	trmD	2270626..2271372 (-)	747	WP_010319524.1	tRNA (guanosine(37)-N1)-methyltransferase TrmD	-
N5E84_RS10400 (N5E84_10400)	rimM	2271400..2271954 (-)	555	WP_013857691.1	ribosome maturation factor RimM	-
N5E84_RS10405 (N5E84_10405)	rpsP	2271984..2272232 (-)	249	WP_010319522.1	30S ribosomal protein S16	-

Sequence

Protein

• Length: 293 a.a.
• Molecular weight: 32904.20 Da
• Isoelectric Point: 7.2157

>NTDB_id=730440 N5E84_RS10370 WP_019281902.1 2267621..2268502(+) (pilD) [Vibrio sp. J502]
MEVFIYYPWLFPLFAALFGLIVGSFLNVVIYRLPKIMEREWRQECADCFPEYHIAPPEGQLTLSLPRSSCPHCDTPIRII
DNIPLLSWLFLKGKCSRCQGPISARYPLIELLTASMSFVVASHFGFSYFSLALLFFTFVLIAATFIDFDTMLLPDKLTLP
LLWAGLSLSLMHISPVSLQDAVIGAIAGYLSLWSVYWLFKLVTGKEGMGYGDFKLLAALGAWLGWQYLPIIILMSSVVGI
VFGLIQLRLQKKGIDKAFPFGPYLAIAGWLTLLWGNDIMSWYLGSIMGLSSWG

Nucleotide

• Length: 882 bp

>NTDB_id=730440 N5E84_RS10370 WP_019281902.1 2267621..2268502(+) (pilD) [Vibrio sp. J502]
ATGGAAGTTTTTATTTATTACCCTTGGCTCTTTCCACTTTTTGCAGCGTTGTTTGGCTTAATCGTGGGGAGTTTTCTCAA
TGTGGTCATTTATCGTCTACCCAAAATCATGGAGCGAGAATGGCGACAAGAGTGCGCTGACTGCTTTCCCGAATATCACA
TTGCGCCACCGGAAGGGCAACTCACGCTCAGTCTCCCCCGTTCTAGCTGCCCACACTGTGATACGCCAATCCGTATTATT
GACAATATTCCGCTACTCAGTTGGCTCTTTTTAAAAGGGAAATGTTCCCGTTGCCAAGGTCCGATCAGTGCACGCTATCC
TTTGATTGAACTGCTCACCGCCAGTATGAGCTTTGTCGTGGCTAGCCACTTCGGTTTTAGTTATTTTTCACTAGCGCTAT
TGTTTTTCACTTTTGTGCTCATCGCGGCGACGTTTATCGATTTCGATACCATGCTATTACCCGACAAGCTGACACTGCCA
CTACTGTGGGCGGGCCTCAGCTTATCATTGATGCACATTAGCCCAGTCAGTTTGCAAGATGCGGTGATCGGCGCCATTGC
TGGCTATCTGAGCTTATGGTCGGTCTATTGGCTGTTTAAACTCGTCACAGGCAAAGAAGGAATGGGTTATGGCGATTTCA
AACTGCTGGCTGCTCTCGGCGCTTGGTTGGGCTGGCAATATCTGCCCATCATCATTCTCATGTCATCTGTGGTTGGCATT
GTCTTTGGCCTAATTCAATTGCGCTTGCAAAAAAAAGGGATCGACAAAGCCTTTCCTTTTGGCCCCTATCTCGCCATTGC
TGGCTGGCTAACCTTGTTGTGGGGTAACGATATTATGAGTTGGTATTTAGGTTCAATTATGGGGTTATCGTCATGGGGTT
AG

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (17-124)

• Type IV leader peptidase family (Peptidase_A24) (135-244)

3D structure

• AlphaFold DB: A0A289G9B4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	77.431	98.294	0.761
pilD	Vibrio campbellii strain DS40M4	76.125	98.635	0.751
pilD	Acinetobacter nosocomialis M2	56.458	92.491	0.522
pilD	Acinetobacter baumannii D1279779	56.089	92.491	0.519
pilD	Neisseria gonorrhoeae MS11	46.552	98.976	0.461