Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   N4P55_RS02175 Genome accession   NZ_CP104408
Coordinates   513537..514865 (+) Length   442 a.a.
NCBI ID   WP_262667954.1    Uniprot ID   -
Organism   Pseudomonas fluorescens strain Ant01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 508537..519865
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N4P55_RS02155 (N4P55_02165) - 508612..511074 (-) 2463 WP_407015984.1 penicillin-binding protein 1A -
  N4P55_RS02160 (N4P55_02170) pilM 511295..511951 (+) 657 WP_064454842.1 type IV pilus assembly protein PilM -
  N4P55_RS02165 (N4P55_02175) - 511948..512499 (+) 552 WP_064450306.1 PilN domain-containing protein -
  N4P55_RS02170 (N4P55_02180) - 512496..513467 (+) 972 WP_262666819.1 pilus assembly protein PilP -
  N4P55_RS02175 (N4P55_02185) pilQ 513537..514865 (+) 1329 WP_262667954.1 type IV pilus secretin PilQ Machinery gene
  N4P55_RS02180 (N4P55_02190) aroK 514870..515388 (+) 519 WP_017138567.1 shikimate kinase AroK -
  N4P55_RS02185 (N4P55_02195) aroB 515513..516613 (+) 1101 WP_053253955.1 3-dehydroquinate synthase -
  N4P55_RS02190 (N4P55_02200) - 516624..518192 (+) 1569 WP_053253956.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 442 a.a.        Molecular weight: 48183.18 Da        Isoelectric Point: 4.8879

>NTDB_id=729666 N4P55_RS02175 WP_262667954.1 513537..514865(+) (pilQ) [Pseudomonas fluorescens strain Ant01]
MAFAVPNRLDLIQLPPPGSSAVNTSYSGDKLNLNFQNIDLRAVLQQIADVAGLNLVASDDVQGSITLRLKEVPWDQALDL
VLQAKGLDKRVKAGVLLVAPAEELAARELLTLESRKQMAELAPLRRELLQVNYAKAADLAKLFQSVTSLEGIPDERGSVA
VDDRTNNIIAYQTGERLEELRRIVAQLDVPVRQVMIEARIVEANVDYDKSLGVRWGGRLNRGKWGAGGINKPSAEGAEPP
ENPPSSPFVDMGSLTGTSGLGIAYITDNLLLDLELTAMEKTGNGEIVSQPKVVTSDKETARILKGTEIPYQESTSRGATS
VSFKEASLSLEVTPQITPDGWVVMEVKVTKDEPDYLNKLNDVPPIKKNEVNAKVLVKDGETIVIGGVFSNTQSKVVDKVP
FLGDVPYLGRLFRRDVLAERKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 1329 bp        

>NTDB_id=729666 N4P55_RS02175 WP_262667954.1 513537..514865(+) (pilQ) [Pseudomonas fluorescens strain Ant01]
ATGGCATTTGCTGTGCCCAATCGCCTGGACCTGATCCAGCTGCCACCTCCCGGCAGCTCGGCGGTGAATACCAGCTATTC
CGGTGACAAGCTGAACCTCAACTTCCAGAACATCGATTTGCGCGCTGTATTGCAGCAGATTGCGGATGTCGCCGGGCTTA
ATCTGGTGGCCAGCGACGATGTGCAAGGCTCCATCACCCTGCGGCTCAAGGAGGTGCCCTGGGATCAGGCACTGGACCTG
GTGTTGCAAGCCAAGGGGTTGGACAAGCGGGTGAAGGCCGGTGTGTTACTGGTGGCGCCGGCCGAAGAGTTGGCCGCCCG
CGAACTGCTGACCCTGGAGTCGCGCAAGCAAATGGCCGAGTTGGCGCCCTTGCGCCGGGAACTGTTGCAGGTCAATTACG
CCAAGGCGGCCGACCTGGCCAAATTGTTCCAATCGGTCACCAGCCTTGAAGGCATCCCCGATGAGCGCGGTTCGGTAGCC
GTGGATGATCGCACCAATAACATTATTGCCTACCAGACCGGCGAACGGCTCGAAGAGCTGCGGCGGATCGTGGCGCAACT
GGATGTCCCGGTGCGCCAGGTGATGATCGAGGCGCGGATCGTTGAAGCCAATGTCGATTACGACAAAAGCCTTGGCGTGC
GCTGGGGTGGGCGCCTGAACCGTGGCAAGTGGGGGGCGGGCGGTATCAACAAACCTTCGGCCGAGGGTGCTGAACCGCCG
GAGAACCCGCCCAGCTCGCCGTTTGTCGACATGGGTTCGCTCACGGGTACTTCAGGCCTGGGTATCGCCTATATCACCGA
CAACCTGTTGCTGGATCTTGAGCTGACGGCCATGGAGAAAACCGGCAACGGTGAAATCGTCTCGCAGCCCAAGGTGGTCA
CCTCCGACAAGGAAACCGCGCGCATCCTCAAGGGCACCGAGATCCCCTATCAGGAATCTACCTCGCGCGGTGCTACATCG
GTGTCGTTCAAGGAAGCCTCGCTGTCGCTGGAGGTGACGCCGCAAATCACCCCGGATGGCTGGGTGGTCATGGAGGTCAA
GGTCACCAAGGACGAGCCCGACTATCTGAACAAGCTCAATGACGTACCGCCGATCAAGAAAAACGAAGTCAACGCCAAGG
TGCTGGTCAAGGATGGCGAGACCATCGTCATCGGTGGCGTTTTCTCCAATACCCAAAGCAAAGTGGTAGATAAAGTGCCA
TTTTTGGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGCGATGTGCTGGCGGAAAGAAAATCCGAGCTGCTGGTATT
CCTGACTCCGCGTATTATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(277-433)

Bacterial type II/III secretion system short domain (Secretin_N)

(128-193)

Secretin and TonB N terminus short domain (STN)

(54-100)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

71.071

99.321

0.706
  pilQ Acinetobacter baumannii D1279779

39.879

100

0.446
  pilQ Acinetobacter baumannii strain A118

39.879

100

0.446
  comQ Acinetobacter baylyi ADP1

40.833

100

0.443
  pilQ Vibrio campbellii strain DS40M4

43.499

95.701

0.416
  pilQ Legionella pneumophila strain Lp02

43.571

95.023

0.414
  pilQ Legionella pneumophila strain ERS1305867

43.095

95.023

0.41
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

42.788

94.118

0.403
  pilQ Vibrio cholerae strain A1552

42.788

94.118

0.403