NTDB

Overview

Name	comEA	Type	Machinery gene
Locus tag	N4R43_RS11475	Genome accession	NZ_CP104388
Coordinates	2279942..2280589 (-)	Length	215 a.a.
NCBI ID	WP_058223210.1	Uniprot ID	A0AAP3Z0X8
Organism	Lactococcus lactis strain LJL7m20
Function	dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2274942..2285589

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
N4R43_RS11450 (N4R43_11465)	atpF	2275011..2275517 (-)	507	WP_010906128.1	F0F1 ATP synthase subunit B	-
N4R43_RS11455 (N4R43_11470)	atpB	2275532..2276245 (-)	714	WP_004255255.1	F0F1 ATP synthase subunit A	-
N4R43_RS11460 (N4R43_11475)	-	2276290..2276505 (-)	216	WP_004255250.1	F0F1 ATP synthase subunit C	-
N4R43_RS11465 (N4R43_11480)	-	2276690..2277466 (-)	777	WP_058206315.1	alpha/beta hydrolase family protein	-
N4R43_RS11470 (N4R43_11485)	comEC	2277751..2279961 (-)	2211	WP_317141310.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
N4R43_RS11475 (N4R43_11490)	comEA	2279942..2280589 (-)	648	WP_058223210.1	ComEA family DNA-binding protein	Machinery gene
N4R43_RS11480 (N4R43_11495)	-	2280649..2282010 (-)	1362	WP_021214597.1	ABC transporter permease	-
N4R43_RS11485 (N4R43_11500)	-	2282007..2282939 (-)	933	WP_004255238.1	ABC transporter ATP-binding protein	-
N4R43_RS11490 (N4R43_11505)	-	2283044..2283442 (-)	399	WP_004255236.1	hypothetical protein	-
N4R43_RS11495 (N4R43_11510)	-	2283551..2284114 (-)	564	WP_004255233.1	GNAT family N-acetyltransferase	-
N4R43_RS11500 (N4R43_11515)	-	2284287..2285465 (-)	1179	WP_012898345.1	SLC13 family permease	-

Sequence

Protein

Download Length: 215 a.a. Molecular weight: 23610.74 Da Isoelectric Point: 4.8659

                                >NTDB_id=729352 N4R43_RS11475 WP_058223210.1 2279942..2280589(-) (comEA) [Lactococcus lactis strain LJL7m20]

MDKILEKVKEYWKMIVLVVCGLIAGGIFYVLTNGQKPTTNLSVENLSSVSRQSSVSKFSEPNEKSVSKIMVDLKGAVTKP

NVYQISSDERLVDLIKEAGGFTDQADQKSINLSAKLKDEEVIYVPKIGENSSTENTDSSANSVTSQVSTTTEKININQAD

VTELQKLTGVGQKKAQDIIDFRTKNGDFKSLEDLGKVSGFGDKTLEKLKDELCFE

Nucleotide

Download Length: 648 bp

                                >NTDB_id=729352 N4R43_RS11475 WP_058223210.1 2279942..2280589(-) (comEA) [Lactococcus lactis strain LJL7m20]

ATGGATAAGATTTTAGAAAAAGTAAAAGAATATTGGAAAATGATTGTTTTAGTTGTTTGTGGGCTCATTGCTGGTGGGAT

TTTTTACGTTTTAACCAACGGTCAAAAGCCAACTACAAATCTGTCAGTAGAAAATTTAAGTTCTGTCAGCAGGCAAAGCT

CTGTCAGTAAATTTAGTGAACCTAATGAAAAATCTGTCAGTAAAATTATGGTTGATTTAAAAGGTGCGGTAACAAAGCCT

AATGTCTATCAAATTTCGTCAGATGAACGTCTAGTTGATCTAATTAAGGAAGCTGGTGGTTTTACTGACCAAGCTGACCA

GAAATCAATTAATCTGTCAGCAAAATTAAAAGATGAAGAAGTAATTTATGTCCCAAAAATTGGTGAAAATTCAAGCACAG

AAAATACTGATAGCTCTGCTAATTCGGTCACTAGTCAAGTCTCAACAACCACTGAAAAAATAAATATCAATCAAGCAGAT

GTGACTGAACTACAAAAATTAACAGGTGTTGGTCAGAAAAAAGCTCAAGACATCATCGATTTTCGGACGAAAAATGGTGA

TTTCAAATCACTTGAAGACTTGGGAAAAGTTTCTGGTTTTGGAGATAAAACACTAGAAAAATTGAAAGATGAGTTGTGTT

TTGAATAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(151-212)

SLBB domain (SLBB)

(70-125)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comEA	Lactococcus lactis subsp. cremoris KW2	75.688	100	0.767
comEA/celA/cilE	Streptococcus mitis SK321	44.444	100	0.447
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	43.119	100	0.437
comEA/celA/cilE	Streptococcus pneumoniae Rx1	41.096	100	0.419
comEA/celA/cilE	Streptococcus pneumoniae D39	41.096	100	0.419
comEA/celA/cilE	Streptococcus pneumoniae R6	41.096	100	0.419
comEA/celA/cilE	Streptococcus mitis NCTC 12261	41.284	100	0.419
comEA	Latilactobacillus sakei subsp. sakei 23K	36.564	100	0.386
comEA	Staphylococcus aureus MW2	37.559	99.07	0.372

Detailed information NTG0729352