Detailed information NTG0728129

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: PALA1_RS25815
• Genome accession: NZ_CP104254
• Coordinates: 5436323..5438023 (+)
• Length: 566 a.a.
• NCBI ID: WP_269972702.1
• Uniprot ID: -
• Organism: Pseudomonas aeruginosa strain PALA1
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5431323..5443023

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PALA1_RS25790 (PALA1_05090)	ampD	5431334..5431900 (-)	567	WP_003104911.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-
PALA1_RS25795 (PALA1_05091)	-	5432051..5434306 (-)	2256	WP_016264271.1	DUF1631 domain-containing protein	-
PALA1_RS25800 (PALA1_05092)	nadC	5434592..5435440 (+)	849	WP_021264134.1	carboxylating nicotinate-nucleotide diphosphorylase	-
PALA1_RS25810	pilA	5435642..5436091 (-)	450	WP_021264133.1	pilin	Machinery gene
PALA1_RS25815 (PALA1_05094)	pilB	5436323..5438023 (+)	1701	WP_269972702.1	type IV-A pilus assembly ATPase PilB	Machinery gene
PALA1_RS25820 (PALA1_05095)	pilC	5438027..5439247 (+)	1221	WP_023101565.1	type 4a pilus biogenesis protein PilC	Machinery gene
PALA1_RS25825 (PALA1_05096)	pilD	5439251..5440123 (+)	873	WP_021264129.1	type IV prepilin peptidase/methyltransferase PilD	Machinery gene
PALA1_RS25830 (PALA1_05097)	coaE	5440120..5440731 (+)	612	WP_003094654.1	dephospho-CoA kinase	-
PALA1_RS25835 (PALA1_05098)	yacG	5440728..5440928 (+)	201	WP_003094656.1	DNA gyrase inhibitor YacG	-
PALA1_RS25840 (PALA1_05099)	-	5440965..5441174 (-)	210	WP_003094660.1	hypothetical protein	-
PALA1_RS25845 (PALA1_05100)	-	5441280..5441969 (-)	690	WP_003094662.1	energy-coupling factor ABC transporter permease	-
PALA1_RS25850 (PALA1_05101)	-	5441966..5442436 (-)	471	WP_003094664.1	hypothetical protein	-
PALA1_RS25855 (PALA1_05102)	-	5442433..5442858 (-)	426	WP_021264128.1	GNAT family N-acetyltransferase	-

Sequence

Protein

• Length: 566 a.a.
• Molecular weight: 62427.28 Da
• Isoelectric Point: 5.5603

>NTDB_id=728129 PALA1_RS25815 WP_269972702.1 5436323..5438023(+) (pilB) [Pseudomonas aeruginosa strain PALA1]
MNDSIQLSGLSRQLVQANLLDEKTALQAQAQAQRNKLSLVTHLVQNKLVSGLALAELSAEQFGIAYCDLNSLDKESFPRD
AISEKLVRQHRVIPLWRRGNKLFVGISDPANHQAINDVQFSTGLTTEAILVEDDKLGLAIDKLFESATDGLAGLDDVDLE
GLDIGSADKSTQEDASAEADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKIYRVRFRTDGMLHEVAKPPIQLASRISARL
KVMAGLDISERRKPQDGRIKMRVSKTKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHEVPRETLLHEGFPE
DKIGTFKLYSPVGCDHCKNDYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQARKEGFNDLRTSGLLKAMQGITSLEEV
NRVTKD

Nucleotide

• Length: 1701 bp

>NTDB_id=728129 PALA1_RS25815 WP_269972702.1 5436323..5438023(+) (pilB) [Pseudomonas aeruginosa strain PALA1]
ATGAACGACAGCATCCAACTGAGCGGCCTGTCCCGACAGCTCGTCCAAGCCAATCTACTCGACGAAAAGACCGCCCTGCA
GGCCCAGGCGCAGGCGCAGCGCAACAAGCTGTCGCTGGTGACGCACCTGGTGCAGAACAAGCTGGTAAGCGGCCTGGCCC
TGGCCGAATTATCCGCCGAGCAGTTCGGTATCGCCTACTGCGACCTGAATAGCCTGGACAAGGAAAGCTTTCCGCGGGAC
GCCATCAGCGAAAAGCTGGTCCGCCAGCACCGGGTCATCCCTTTGTGGCGCCGCGGCAACAAGCTCTTTGTAGGCATCTC
CGATCCCGCCAACCACCAGGCCATCAATGATGTCCAGTTCAGCACCGGCCTGACGACCGAAGCGATTCTGGTCGAAGACG
ACAAGCTTGGCCTGGCCATCGACAAGCTGTTCGAAAGCGCTACCGACGGTCTCGCAGGCCTCGACGATGTCGACCTCGAG
GGACTGGATATCGGTAGCGCGGACAAGTCCACCCAAGAGGATGCCAGCGCAGAAGCGGACGACGCACCTGTAGTACGTTT
CGTCAACAAGATGCTGCTGGATGCCATCAAGGGCGGCTCTTCCGACCTGCACTTCGAGCCCTACGAGAAGATCTACCGAG
TGCGCTTCCGTACCGACGGCATGCTCCACGAAGTGGCCAAGCCGCCGATCCAGTTGGCCAGTCGTATTTCCGCGCGACTC
AAGGTGATGGCCGGCCTGGATATCTCCGAACGACGCAAGCCGCAGGACGGACGGATCAAGATGCGCGTGTCGAAGACCAA
GTCCATCGACTTCCGCGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGATCGTGATGCGGATCCTCGACTCCTCCAGCG
CACAGATGGGCATCGACGCCCTCGGCTACGAGGAGGACCAGAAGGAACTCTACCTGGCCGCACTCAAGCAGCCGCAGGGC
ATGATCCTGGTCACCGGCCCCACCGGCTCGGGCAAGACGGTATCGCTATACACCGGCCTGAACATCCTGAATACCACCGA
CATCAACATTTCCACCGCCGAAGACCCGGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAATCCGCGCCAGG
GCATGGACTTCTCCCAGGCGCTGCGCGCCTTCCTGCGCCAGGACCCGGATGTGATCATGGTCGGCGAGATCCGCGACCTG
GAGACCGCCGAGATCGCCATCAAGGCGGCGCAGACCGGGCATATGGTGATGTCCACCCTGCACACCAACAGCGCCGCCGA
GACCCTGACCCGGTTGCTGAACATGGGCGTGCCGGCGTTCAACCTGGCGACCTCGGTGAACCTGATCATCGCCCAGCGCC
TTGCGCGAAAACTCTGTTCGCACTGCAAGAAAGAGCACGAGGTGCCAAGGGAAACCCTGCTTCACGAGGGCTTCCCGGAA
GACAAGATCGGCACCTTCAAGCTTTATTCGCCGGTGGGCTGCGACCATTGCAAGAACGATTACAAGGGACGTGTCGGTAT
TTATGAAGTAGTTAAAAACACCCCGGCCCTGCAGCGGATTATCATGGAGGAAGGCAACTCCATCGAGATCGCCGAGCAAG
CCCGCAAAGAAGGCTTCAACGATCTGCGCACCTCAGGCCTGCTGAAAGCCATGCAGGGGATCACCAGCCTGGAGGAAGTC
AACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (43-147)

• Type II/IV secretion system protein (T2SSE) (190-457)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baumannii D1279779	57.343	100	0.58
pilB	Acinetobacter baylyi ADP1	57.016	99.47	0.567
pilB	Legionella pneumophila strain ERS1305867	54.947	100	0.549
pilB	Vibrio parahaemolyticus RIMD 2210633	50.709	99.647	0.505
pilF	Neisseria gonorrhoeae MS11	50.356	99.293	0.5
pilB	Vibrio cholerae strain A1552	49.823	99.647	0.496
pilB	Vibrio campbellii strain DS40M4	49.468	99.647	0.493
pilF	Thermus thermophilus HB27	40.072	97.88	0.392
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.228	93.11	0.375
pilB/pilB1	Synechocystis sp. PCC 6803	38.577	94.346	0.364