Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   M2494_RS23310 Genome accession   NZ_AP019411
Coordinates   5226393..5227610 (-) Length   405 a.a.
NCBI ID   WP_003381202.1    Uniprot ID   A0A2V0Q5P8
Organism   Pseudomonas syringae pv. actinidiae strain MAFF613020     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5221393..5232610
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M2494_RS23275 (KPSA1B_104745) - 5221912..5222313 (+) 402 Protein_4604 transporter -
  M2494_RS23280 - 5222375..5223552 (+) 1178 WP_164706461.1 IS3 family transposase -
  M2494_RS23285 - 5223605..5223811 (+) 207 Protein_4606 transporter -
  M2494_RS23290 - 5224101..5224427 (+) 327 WP_019334445.1 hypothetical protein -
  M2494_RS23295 (KPSA1B_104748) yacG 5224693..5224902 (-) 210 WP_003381207.1 DNA gyrase inhibitor YacG -
  M2494_RS23300 (KPSA1B_104749) coaE 5224899..5225522 (-) 624 WP_003381205.1 dephospho-CoA kinase -
  M2494_RS23305 (KPSA1B_104750) pilD 5225519..5226391 (-) 873 WP_003381203.1 A24 family peptidase Machinery gene
  M2494_RS23310 (KPSA1B_104751) pilC 5226393..5227610 (-) 1218 WP_003381202.1 type II secretion system F family protein Machinery gene
  M2494_RS23315 (KPSA1B_104752) pilB 5227613..5229307 (-) 1695 WP_003381201.1 type IV-A pilus assembly ATPase PilB Machinery gene
  M2494_RS23320 - 5229533..5229925 (+) 393 WP_003381200.1 pilin -
  M2494_RS23325 (KPSA1B_104753) - 5229971..5231398 (+) 1428 WP_017699050.1 O-antigen ligase family protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44238.16 Da        Isoelectric Point: 10.1599

>NTDB_id=72633 M2494_RS23310 WP_003381202.1 5226393..5227610(-) (pilC) [Pseudomonas syringae pv. actinidiae strain MAFF613020]
MASKAVKVSTYTWEGVDKKGGKLSGEVSGHNLALVKAQLRKQGINFTKVRKKPVSIFGKGKKIKPLDIAFFSRQMATMMK
AGVPLLQSFDIIAEGAENPNMRALVGSLKQEVSAGNSFATALRKKPEYFDDLFCNLVDAGEQAGALESLLDRVASYKEKT
ERLKAKIKKAMTYPTAVLIVAFIVSGILLIKVVPQFQSVFAGFNAELPAFTLMVIALSEVVQQWWLAIVALFFVSFFMFK
RAYKQSQKFRDSLDRFLLKVPIIGPLIFKSSVARYARTLATTFAAGVPLVEALDSVAGATGNVVFRNAVNQVKQDVSTGM
QLNFSMRSTGVFPSLAIQMTAIGEESGALDAMLDKVATYYEEEVDNMVDNMTSLMEPMIMAILGVIVGGLVIAMYLPIFK
LGSVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=72633 M2494_RS23310 WP_003381202.1 5226393..5227610(-) (pilC) [Pseudomonas syringae pv. actinidiae strain MAFF613020]
ATGGCCAGCAAGGCAGTTAAAGTCAGTACTTACACATGGGAAGGTGTGGACAAGAAAGGCGGCAAACTCAGTGGAGAAGT
GAGCGGGCACAACCTCGCGCTCGTGAAGGCTCAGTTACGCAAGCAAGGAATCAATTTCACCAAAGTACGTAAAAAGCCCG
TATCGATTTTTGGCAAAGGTAAGAAGATCAAGCCGCTGGACATCGCATTCTTTTCCCGGCAGATGGCTACCATGATGAAG
GCAGGAGTGCCACTGCTGCAGTCATTTGACATCATTGCCGAAGGTGCAGAAAACCCGAACATGCGTGCGCTGGTCGGCTC
TCTAAAGCAGGAGGTTTCAGCAGGGAACAGCTTTGCGACGGCTCTGCGAAAGAAACCGGAATATTTCGACGACCTGTTCT
GCAATCTGGTCGATGCCGGTGAGCAAGCGGGGGCGCTCGAAAGCCTGCTGGACCGCGTGGCGAGCTACAAGGAAAAGACG
GAAAGGCTAAAGGCCAAAATCAAGAAGGCGATGACTTATCCTACCGCCGTATTAATTGTTGCTTTTATCGTGTCAGGCAT
TTTGCTGATTAAGGTCGTGCCGCAGTTCCAGTCTGTTTTCGCTGGTTTTAACGCAGAATTACCAGCGTTCACGCTGATGG
TCATCGCCCTTTCGGAAGTCGTTCAACAGTGGTGGCTGGCAATAGTTGCGTTATTTTTTGTCAGCTTTTTCATGTTCAAG
CGGGCTTACAAACAGTCTCAAAAGTTTCGTGACAGCCTCGACCGCTTCCTTCTGAAAGTCCCCATTATCGGACCACTGAT
TTTCAAGTCGTCCGTCGCGCGCTATGCGCGGACCCTTGCGACAACCTTTGCCGCAGGTGTCCCGCTGGTGGAGGCACTTG
ACTCGGTCGCCGGCGCCACCGGTAACGTCGTATTCAGGAACGCAGTCAACCAGGTCAAACAGGATGTCTCCACTGGTATG
CAATTGAATTTCTCGATGCGTTCAACGGGTGTATTTCCAAGTCTGGCGATACAAATGACAGCAATCGGCGAAGAGTCCGG
TGCGCTTGATGCCATGCTCGATAAAGTCGCGACCTACTATGAAGAAGAAGTCGACAACATGGTCGACAATATGACCAGCC
TGATGGAACCGATGATCATGGCGATACTGGGTGTCATCGTCGGCGGCCTGGTTATTGCCATGTACCTCCCCATCTTTAAA
CTCGGAAGCGTTGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2V0Q5P8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

73.333

100

0.733
  pilC Acinetobacter baylyi ADP1

59.314

100

0.598
  pilC Acinetobacter baumannii D1279779

59.406

99.753

0.593
  pilC Legionella pneumophila strain ERS1305867

52.897

98.025

0.519
  pilG Neisseria gonorrhoeae MS11

42.68

99.506

0.425
  pilG Neisseria meningitidis 44/76-A

42.68

99.506

0.425
  pilC Vibrio campbellii strain DS40M4

41.769

100

0.42
  pilC Vibrio cholerae strain A1552

41.481

100

0.415
  pilC Thermus thermophilus HB27

37.905

99.012

0.375

Multiple sequence alignment