Detailed information NTG0072632

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: M2494_RS23305
• Genome accession: NZ_AP019411
• Coordinates: 5225519..5226391 (-)
• Length: 290 a.a.
• NCBI ID: WP_003381203.1
• Uniprot ID: A0A2V0QGS2
• Organism: Pseudomonas syringae pv. actinidiae strain MAFF613020
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5220519..5231391

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M2494_RS23265 (KPSA1B_104743)	-	5220567..5221100 (-)	534	WP_003381215.1	MOSC domain-containing protein	-
M2494_RS23270 (KPSA1B_104744)	-	5221097..5221726 (-)	630	WP_110459543.1	DUF1780 domain-containing protein	-
M2494_RS23275 (KPSA1B_104745)	-	5221912..5222313 (+)	402	Protein_4604	transporter	-
M2494_RS23280	-	5222375..5223552 (+)	1178	WP_164706461.1	IS3 family transposase	-
M2494_RS23285	-	5223605..5223811 (+)	207	Protein_4606	transporter	-
M2494_RS23290	-	5224101..5224427 (+)	327	WP_019334445.1	hypothetical protein	-
M2494_RS23295 (KPSA1B_104748)	yacG	5224693..5224902 (-)	210	WP_003381207.1	DNA gyrase inhibitor YacG	-
M2494_RS23300 (KPSA1B_104749)	coaE	5224899..5225522 (-)	624	WP_003381205.1	dephospho-CoA kinase	-
M2494_RS23305 (KPSA1B_104750)	pilD	5225519..5226391 (-)	873	WP_003381203.1	A24 family peptidase	Machinery gene
M2494_RS23310 (KPSA1B_104751)	pilC	5226393..5227610 (-)	1218	WP_003381202.1	type II secretion system F family protein	Machinery gene
M2494_RS23315 (KPSA1B_104752)	pilB	5227613..5229307 (-)	1695	WP_003381201.1	type IV-A pilus assembly ATPase PilB	Machinery gene
M2494_RS23320	-	5229533..5229925 (+)	393	WP_003381200.1	pilin	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 32020.33 Da
• Isoelectric Point: 8.3782

>NTDB_id=72632 M2494_RS23305 WP_003381203.1 5225519..5226391(-) (pilD) [Pseudomonas syringae pv. actinidiae strain MAFF613020]
MPLLDLLASSPLAFVTTCCLLGLIVGSFLNVVVYRLPKMMERDWKAQSREMLGLPAVPDQPVFNLILPHSSCPHCAHKIR
PWENLPIISYLLLRGKCSHCKAPISKRYPLVELTCAVLSAYVAWHFGFGWQAAAMLVLSWGLLAMSLIDADHQLLPDSLV
LPLLWLGLIVNAFGLFTSLNDALWGAVAGYLTLWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLMG
AVLGVIMMRVRRVESGTPIPFGPYLAIAGWIALLWGGQITDSYMQFAGFR

Nucleotide

• Length: 873 bp

>NTDB_id=72632 M2494_RS23305 WP_003381203.1 5225519..5226391(-) (pilD) [Pseudomonas syringae pv. actinidiae strain MAFF613020]
ATGCCCCTCCTCGACCTCCTGGCAAGCTCACCCCTGGCCTTCGTCACCACCTGCTGCCTGCTCGGCCTCATCGTCGGAAG
CTTCCTCAACGTAGTCGTTTACCGCCTCCCAAAAATGATGGAGCGCGACTGGAAAGCGCAATCACGGGAAATGCTCGGCC
TCCCTGCCGTACCGGACCAACCCGTCTTCAACCTGATCCTGCCGCACTCCAGTTGCCCGCACTGCGCGCACAAGATCCGC
CCATGGGAAAATCTGCCGATCATCAGTTACCTGTTGCTGCGCGGCAAATGCTCACACTGCAAAGCACCGATCAGCAAGCG
CTACCCATTGGTAGAGCTGACCTGCGCAGTGTTATCGGCCTACGTCGCCTGGCACTTCGGCTTTGGCTGGCAGGCGGCTG
CGATGCTGGTGTTGAGCTGGGGTTTGCTGGCGATGAGTCTGATCGATGCCGATCATCAGCTGTTGCCTGACTCTCTGGTA
CTTCCACTGCTGTGGCTGGGCCTGATCGTTAACGCCTTTGGGCTGTTCACCTCGCTGAATGATGCGTTGTGGGGCGCAGT
GGCCGGTTATCTGACGCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACGGGCAAGGAAGGCATGGGCTATGGCGACT
TCAAGTTGCTGGCCATGCTTGGCGCGTGGGGTGGCTGGCAGATTCTGCCGTTGACCATTCTGTTGTCGTCGCTGATGGGT
GCGGTGCTGGGGGTGATCATGATGCGCGTGCGCAGGGTCGAAAGCGGTACGCCGATCCCCTTTGGTCCTTATCTGGCCAT
TGCGGGGTGGATCGCTTTGCTCTGGGGTGGTCAAATAACCGACTCTTACATGCAGTTTGCCGGTTTCAGATGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (136-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

3D structure

• AlphaFold DB: A0A2V0QGS2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	54.417	97.586	0.531
pilD	Vibrio campbellii strain DS40M4	51.237	97.586	0.5
pilD	Acinetobacter baumannii D1279779	51.625	95.517	0.493
pilD	Acinetobacter nosocomialis M2	51.264	95.517	0.49
pilD	Neisseria gonorrhoeae MS11	52.239	92.414	0.483