NTDB

Overview

Name	pilB	Type	Machinery gene
Locus tag	NYR63_RS04775	Genome accession	NZ_CP103834
Coordinates	1056589..1057983 (-)	Length	464 a.a.
NCBI ID	WP_279458427.1	Uniprot ID	-
Organism	Actinobacillus genomosp. 1 strain 3216
Function	type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1051589..1062983

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NYR63_RS04745 (NYR63_04740)	lptE	1052212..1052706 (+)	495	WP_279458421.1	LPS assembly lipoprotein LptE	-
NYR63_RS04750 (NYR63_04745)	holA	1052808..1053833 (+)	1026	WP_279458422.1	DNA polymerase III subunit delta	-
NYR63_RS04755 (NYR63_04750)	yacG	1053909..1054097 (-)	189	WP_279458423.1	DNA gyrase inhibitor YacG	-
NYR63_RS04760 (NYR63_04755)	coaE	1054079..1054717 (-)	639	WP_279458424.1	dephospho-CoA kinase	-
NYR63_RS04765 (NYR63_04760)	-	1054738..1055382 (-)	645	WP_347710369.1	prepilin peptidase	-
NYR63_RS04770 (NYR63_04765)	-	1055403..1056605 (-)	1203	WP_279458426.1	type II secretion system F family protein	-
NYR63_RS04775 (NYR63_04770)	pilB	1056589..1057983 (-)	1395	WP_279458427.1	GspE/PulE family protein	Machinery gene
NYR63_RS04780 (NYR63_04775)	pilA	1058001..1058447 (-)	447	WP_279458428.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	Machinery gene
NYR63_RS04785 (NYR63_04780)	radA	1058627..1060006 (+)	1380	WP_279458429.1	DNA repair protein RadA	Machinery gene
NYR63_RS04790 (NYR63_04785)	-	1060050..1060283 (-)	234	WP_115589814.1	YgjV family protein	-
NYR63_RS04795 (NYR63_04790)	folA	1060413..1060901 (-)	489	WP_279458430.1	type 3 dihydrofolate reductase	-
NYR63_RS04800 (NYR63_04795)	proB	1060988..1062079 (+)	1092	WP_279458431.1	glutamate 5-kinase	-
NYR63_RS04805 (NYR63_04800)	-	1062095..1062871 (+)	777	WP_279458432.1	TSUP family transporter	-

Sequence

Protein

Download Length: 464 a.a. Molecular weight: 52724.35 Da Isoelectric Point: 5.7445

                                >NTDB_id=725817 NYR63_RS04775 WP_279458427.1 1056589..1057983(-) (pilB) [Actinobacillus genomosp. 1 strain 3216]

MQYSVCDINSRQVLRISDERWQKNAEEGHILLRYLAVPIQEDEQILWLAIDDMNNLSACEIFAFIAHKRIEPVLVSSEEL

KYLLGQLMPEQQSVYEETELHYQSLEQTEHLDFNDPIIQVLDSLFKYSLKHNVSDIHLEPQKDNMLIRLRIDGVLHHYKS

LSLQLANRILSRIKLLAKLDISESRLPQDGQFSFKTALSETLDFRVSSLPTNWGEKVVLRLQKNKPTHFDFISLGFLAEQ

QEKLLQALRQPQGLILVTGPTGSGKSITLYSALDYLNDSKRHILTAEDPIEIELDGIIQTQVNPAINLGFSRLLRTFLRQ

DPDVIMLGEIRDQESAEIALSAAQTGHLVLSTLHTNDAPSAIERLLQLGIKEYEINNSLLLVIAQRLVRKLCTQCGGTGC

KHCYHGYNGRIGVYQMFSKNGKIFEKSTACLDFENLYQSAQQKLNAGVTSLEEINRVLGNVESI

Nucleotide

Download Length: 1395 bp

                                >NTDB_id=725817 NYR63_RS04775 WP_279458427.1 1056589..1057983(-) (pilB) [Actinobacillus genomosp. 1 strain 3216]

ATGCAGTATTCGGTATGTGACATAAACAGTAGGCAAGTATTGCGGATCTCCGATGAACGATGGCAAAAAAATGCTGAGGA

AGGTCATATTCTATTACGTTATCTTGCCGTACCGATTCAGGAAGATGAGCAAATCTTGTGGCTTGCAATCGACGATATGA

ATAATCTCAGTGCTTGCGAAATTTTTGCTTTTATTGCTCATAAGCGAATTGAACCGGTATTGGTTTCCTCCGAAGAATTG

AAATATCTACTTGGACAATTAATGCCGGAACAACAGTCCGTATATGAAGAAACCGAACTGCATTATCAGTCTTTAGAACA

AACGGAACATTTGGATTTTAATGATCCTATTATTCAAGTGTTAGATAGCCTGTTTAAATACAGTTTAAAGCACAATGTCT

CGGATATTCATTTAGAACCGCAGAAAGACAATATGCTGATTCGATTACGTATTGACGGTGTGTTACATCACTATAAATCA

CTTTCTCTTCAGCTTGCCAACCGTATTCTTTCTCGAATTAAATTATTAGCGAAACTCGATATTAGCGAATCTCGTCTTCC

GCAAGACGGGCAATTTAGTTTTAAAACCGCATTATCAGAAACATTGGATTTTCGCGTTTCAAGTTTACCGACTAATTGGG

GAGAGAAAGTAGTTTTACGTTTACAAAAAAATAAGCCTACGCATTTCGATTTTATTTCCTTAGGCTTTTTAGCGGAACAG

CAAGAAAAACTATTACAGGCACTGCGACAACCCCAAGGGCTAATACTGGTAACAGGGCCGACGGGCAGCGGTAAGAGCAT

TACGTTATACAGCGCATTAGATTATTTAAATGACAGTAAAAGGCATATTTTGACCGCGGAAGATCCTATTGAAATCGAGC

TTGATGGAATTATTCAAACGCAAGTAAATCCTGCGATAAATTTGGGTTTTAGTCGGTTATTGAGGACGTTTTTACGTCAA

GATCCGGATGTGATTATGTTAGGGGAGATTCGTGATCAAGAAAGTGCCGAAATAGCGTTGAGTGCGGCGCAAACCGGGCA

TTTGGTTCTCTCTACTTTGCACACTAATGATGCGCCTTCGGCTATTGAACGTTTATTACAACTCGGTATAAAAGAATATG

AAATCAATAATTCGTTATTACTTGTTATAGCGCAACGTTTAGTACGTAAATTATGTACTCAATGCGGCGGGACAGGATGT

AAACATTGTTATCACGGTTACAACGGGAGAATCGGCGTTTATCAGATGTTTTCAAAAAATGGCAAAATTTTTGAAAAATC

GACCGCTTGTCTGGATTTTGAAAATTTGTATCAAAGTGCGCAACAGAAACTAAACGCCGGCGTGACTTCATTGGAAGAAA

TTAACAGAGTATTAGGTAATGTTGAAAGTATATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(128-390)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(30-92)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilB	Glaesserella parasuis strain SC1401	70.087	98.707	0.692
pilB	Haemophilus influenzae Rd KW20	58.093	97.198	0.565
pilB	Haemophilus influenzae 86-028NP	57.65	97.198	0.56
pilB	Acinetobacter baumannii D1279779	40.292	100	0.416
pilB	Acinetobacter baylyi ADP1	38.153	100	0.409
pilB	Vibrio campbellii strain DS40M4	39.248	100	0.405
pilB	Vibrio parahaemolyticus RIMD 2210633	39.04	100	0.403
pilB	Vibrio cholerae strain A1552	38.877	100	0.403
pilB	Legionella pneumophila strain ERS1305867	38.542	100	0.399
pilF	Neisseria gonorrhoeae MS11	35.714	100	0.366
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	36.111	100	0.364

Detailed information NTG0725817