Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NYR63_RS04780 Genome accession   NZ_CP103834
Coordinates   1058001..1058447 (-) Length   148 a.a.
NCBI ID   WP_279458428.1    Uniprot ID   -
Organism   Actinobacillus genomosp. 1 strain 3216     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1053001..1063447
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYR63_RS04755 (NYR63_04750) yacG 1053909..1054097 (-) 189 WP_279458423.1 DNA gyrase inhibitor YacG -
  NYR63_RS04760 (NYR63_04755) coaE 1054079..1054717 (-) 639 WP_279458424.1 dephospho-CoA kinase -
  NYR63_RS04765 (NYR63_04760) - 1054738..1055382 (-) 645 WP_347710369.1 prepilin peptidase -
  NYR63_RS04770 (NYR63_04765) - 1055403..1056605 (-) 1203 WP_279458426.1 type II secretion system F family protein -
  NYR63_RS04775 (NYR63_04770) pilB 1056589..1057983 (-) 1395 WP_279458427.1 GspE/PulE family protein Machinery gene
  NYR63_RS04780 (NYR63_04775) pilA 1058001..1058447 (-) 447 WP_279458428.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  NYR63_RS04785 (NYR63_04780) radA 1058627..1060006 (+) 1380 WP_279458429.1 DNA repair protein RadA Machinery gene
  NYR63_RS04790 (NYR63_04785) - 1060050..1060283 (-) 234 WP_115589814.1 YgjV family protein -
  NYR63_RS04795 (NYR63_04790) folA 1060413..1060901 (-) 489 WP_279458430.1 type 3 dihydrofolate reductase -
  NYR63_RS04800 (NYR63_04795) proB 1060988..1062079 (+) 1092 WP_279458431.1 glutamate 5-kinase -
  NYR63_RS04805 (NYR63_04800) - 1062095..1062871 (+) 777 WP_279458432.1 TSUP family transporter -

Sequence

Protein


Download         Length: 148 a.a.        Molecular weight: 16022.42 Da        Isoelectric Point: 8.0852

>NTDB_id=725818 NYR63_RS04780 WP_279458428.1 1058001..1058447(-) (pilA) [Actinobacillus genomosp. 1 strain 3216]
MQKLNLIRPLTKAFTLIELMIVIAIIAILATVAIPSYNSYTQKAALSELLSASASYKTDVEICMYNTGEAKNCSSGQNGV
SKITELKRAKYLNTITVESGTITVTGKGNLQEYGYTMTPNYNGNTISWETKCNGADLSLFPANFCAAN

Nucleotide


Download         Length: 447 bp        

>NTDB_id=725818 NYR63_RS04780 WP_279458428.1 1058001..1058447(-) (pilA) [Actinobacillus genomosp. 1 strain 3216]
ATGCAAAAACTAAATCTTATTCGACCGCTAACTAAAGCATTTACTTTAATTGAATTGATGATCGTGATTGCGATTATCGC
CATTCTTGCTACGGTTGCGATTCCGTCATACAACAGTTATACCCAAAAAGCGGCACTTTCCGAATTGTTATCGGCGTCGG
CTTCCTATAAAACGGATGTTGAAATTTGTATGTATAACACGGGAGAGGCGAAAAATTGTAGTAGCGGTCAGAACGGCGTT
AGCAAAATTACGGAGCTTAAAAGAGCTAAATATCTAAATACGATTACGGTTGAAAGCGGCACGATTACAGTGACGGGGAA
GGGGAATCTACAGGAATACGGCTATACAATGACACCGAACTATAACGGGAATACGATTTCTTGGGAAACGAAATGTAACG
GGGCAGATTTGAGTTTATTTCCGGCAAATTTCTGTGCCGCAAATTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(12-33)

Pilin (bacterial filament) (Pilin)

(43-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

70.47

100

0.709
  pilA Haemophilus influenzae 86-028NP

54.422

99.324

0.541
  pilA Haemophilus influenzae Rd KW20

53.741

99.324

0.534