Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NYR89_RS02705 Genome accession   NZ_CP103833
Coordinates   549937..551328 (+) Length   463 a.a.
NCBI ID   WP_279446233.1    Uniprot ID   -
Organism   Actinobacillus arthritidis strain CCUG24862     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 544937..556328
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYR89_RS02675 (NYR89_02675) - 545044..545817 (-) 774 WP_279446227.1 TSUP family transporter -
  NYR89_RS02680 (NYR89_02680) proB 545833..546924 (-) 1092 WP_279446228.1 glutamate 5-kinase -
  NYR89_RS02685 (NYR89_02685) folA 547009..547497 (+) 489 WP_279446229.1 type 3 dihydrofolate reductase -
  NYR89_RS02690 (NYR89_02690) - 547622..547855 (+) 234 WP_279446230.1 YgjV family protein -
  NYR89_RS02695 (NYR89_02695) radA 547900..549279 (-) 1380 WP_279446231.1 DNA repair protein RadA -
  NYR89_RS02700 (NYR89_02700) pilA 549471..549911 (+) 441 WP_279446232.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  NYR89_RS02705 (NYR89_02705) pilB 549937..551328 (+) 1392 WP_279446233.1 GspE/PulE family protein Machinery gene
  NYR89_RS02710 (NYR89_02710) pilC 551315..552517 (+) 1203 WP_279446234.1 type II secretion system F family protein Machinery gene
  NYR89_RS02715 (NYR89_02715) - 552520..553188 (+) 669 WP_279446235.1 prepilin peptidase -
  NYR89_RS02720 (NYR89_02720) coaE 553210..553847 (+) 638 Protein_525 dephospho-CoA kinase -
  NYR89_RS02725 (NYR89_02725) yacG 553832..554023 (+) 192 WP_279446236.1 DNA gyrase inhibitor YacG -
  NYR89_RS02730 (NYR89_02730) - 554091..554921 (-) 831 WP_279446237.1 serine hydrolase -
  NYR89_RS02735 (NYR89_02735) - 555005..556042 (-) 1038 WP_279446238.1 glycosyltransferase family 9 protein -

Sequence

Protein


Download         Length: 463 a.a.        Molecular weight: 52436.25 Da        Isoelectric Point: 6.5644

>NTDB_id=725798 NYR89_RS02705 WP_279446233.1 549937..551328(+) (pilB) [Actinobacillus arthritidis strain CCUG24862]
MPYSVCDVNSQQAFEISDELWQKNSNERHILLRYLAVPVQENQHTLWLAIDDMKNLSACEIFAFIAHKQIEPVLVSSDEL
KYLLSQLMPEQHSVYEETELPYQSLVASENLDVNDPIIRSLDNLFKYSLKHNVSDIHIEPQKEKIIIRLRIDGVLYHYKS
LSRQLSERVISRIKLLAKLDISESRLPQDGQFSFKTALSETLDFRVSSLPTHWGEKIVLRLQKNKPTHFDFLTLGFLAEQ
KAKLLKALQQPQGLILVTGPTGSGKSITLYSALNYLNDSSRHIMTAEDPVEIEIEGIIQTQVNSAIGLDFSRLLRTFLRQ
DPDVIMLGEIRDRESAEIALRAAQTGHLVLSTLHTNDAPSAIERLLQLGIKEYEIRNALLLVIAQRLVRKLCCKCAGNGC
KTCYQGYKGREGIYQILAKNGEIFDKNTADLDFKTLHQSAEQKVQAGITSLVEVKRVLGDAEA

Nucleotide


Download         Length: 1392 bp        

>NTDB_id=725798 NYR89_RS02705 WP_279446233.1 549937..551328(+) (pilB) [Actinobacillus arthritidis strain CCUG24862]
ATGCCATATTCAGTTTGTGATGTTAATAGCCAACAGGCGTTTGAAATTTCCGATGAACTTTGGCAAAAGAATTCTAATGA
ACGTCATATTTTATTACGTTATCTCGCTGTACCGGTTCAGGAGAATCAACACACTTTATGGTTGGCAATAGATGATATGA
AAAATTTAAGTGCTTGTGAAATTTTTGCATTTATTGCACATAAACAAATTGAGCCGGTATTGGTTTCCTCCGATGAATTA
AAATATCTTCTAAGTCAACTAATGCCGGAACAACATTCTGTTTATGAAGAAACCGAGTTACCTTACCAATCTTTAGTCGC
ATCGGAAAATTTAGACGTGAATGATCCGATTATCCGGAGTTTAGATAATCTTTTTAAATATAGTTTAAAACATAATGTAT
CAGATATTCATATTGAACCGCAAAAAGAAAAGATCATCATTAGATTACGTATTGATGGTGTTCTTTATCATTATAAATCG
CTCTCAAGACAACTTTCTGAGCGAGTTATTTCCAGAATTAAGTTATTGGCGAAATTAGATATTAGTGAATCTCGCCTACC
ACAAGACGGGCAATTTAGCTTTAAAACAGCATTATCTGAAACACTCGATTTTCGGGTATCAAGTTTACCGACACATTGGG
GAGAGAAGATTGTTTTACGTTTACAAAAAAATAAACCGACGCATTTTGATTTTCTTACGTTAGGTTTTTTAGCGGAGCAG
AAAGCAAAGTTATTGAAAGCATTACAACAACCGCAAGGATTAATTTTAGTGACTGGACCTACTGGTAGCGGCAAAAGTAT
TACTTTATATAGTGCATTAAATTATTTAAATGACAGTTCCAGACATATTATGACTGCAGAAGATCCAGTAGAAATTGAAA
TCGAAGGGATCATTCAAACTCAAGTCAATTCGGCGATAGGGTTGGATTTTAGTCGTTTATTAAGAACTTTTTTACGCCAA
GATCCTGATGTGATTATGTTGGGCGAAATTAGGGATAGAGAAAGTGCGGAAATTGCACTACGTGCGGCACAAACCGGACA
TTTAGTGCTTTCAACGTTACATACGAATGATGCACCTTCCGCAATAGAACGTTTATTACAGCTAGGCATAAAAGAATATG
AGATACGTAATGCATTGCTATTAGTGATTGCTCAGCGTTTAGTCCGAAAATTGTGTTGTAAATGTGCCGGCAATGGTTGT
AAGACGTGTTATCAAGGCTATAAAGGGCGTGAAGGCATTTATCAAATTTTAGCCAAAAATGGTGAGATATTTGATAAAAA
CACAGCTGATTTGGACTTTAAAACGTTACATCAAAGTGCGGAGCAAAAAGTCCAAGCGGGTATTACCTCGTTAGTTGAAG
TAAAAAGAGTATTAGGCGATGCAGAAGCATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(127-390)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(13-93)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

70.678

98.704

0.698
  pilB Haemophilus influenzae Rd KW20

58

97.192

0.564
  pilB Haemophilus influenzae 86-028NP

57.589

96.76

0.557
  pilB Acinetobacter baylyi ADP1

40.082

100

0.423
  pilB Vibrio cholerae strain A1552

40.664

100

0.423
  pilB Vibrio parahaemolyticus RIMD 2210633

40.329

100

0.423
  pilB Vibrio campbellii strain DS40M4

40.125

100

0.417
  pilB Legionella pneumophila strain ERS1305867

39.388

100

0.417
  pilB Acinetobacter baumannii D1279779

38.493

100

0.408
  pilF Neisseria gonorrhoeae MS11

36.998

100

0.378
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.111

100

0.365