Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NYR89_RS02700 Genome accession   NZ_CP103833
Coordinates   549471..549911 (+) Length   146 a.a.
NCBI ID   WP_279446232.1    Uniprot ID   -
Organism   Actinobacillus arthritidis strain CCUG24862     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 544471..554911
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYR89_RS02675 (NYR89_02675) - 545044..545817 (-) 774 WP_279446227.1 TSUP family transporter -
  NYR89_RS02680 (NYR89_02680) proB 545833..546924 (-) 1092 WP_279446228.1 glutamate 5-kinase -
  NYR89_RS02685 (NYR89_02685) folA 547009..547497 (+) 489 WP_279446229.1 type 3 dihydrofolate reductase -
  NYR89_RS02690 (NYR89_02690) - 547622..547855 (+) 234 WP_279446230.1 YgjV family protein -
  NYR89_RS02695 (NYR89_02695) radA 547900..549279 (-) 1380 WP_279446231.1 DNA repair protein RadA -
  NYR89_RS02700 (NYR89_02700) pilA 549471..549911 (+) 441 WP_279446232.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  NYR89_RS02705 (NYR89_02705) pilB 549937..551328 (+) 1392 WP_279446233.1 GspE/PulE family protein Machinery gene
  NYR89_RS02710 (NYR89_02710) pilC 551315..552517 (+) 1203 WP_279446234.1 type II secretion system F family protein Machinery gene
  NYR89_RS02715 (NYR89_02715) - 552520..553188 (+) 669 WP_279446235.1 prepilin peptidase -
  NYR89_RS02720 (NYR89_02720) coaE 553210..553847 (+) 638 Protein_525 dephospho-CoA kinase -
  NYR89_RS02725 (NYR89_02725) yacG 553832..554023 (+) 192 WP_279446236.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15887.17 Da        Isoelectric Point: 8.0875

>NTDB_id=725797 NYR89_RS02700 WP_279446232.1 549471..549911(+) (pilA) [Actinobacillus arthritidis strain CCUG24862]
MQKIRLIRPLAKAFTLIELMIVIAIIAILATIAIPSYNSYTQKAALSELLSASSSYKTDVEICVYNTGDINACNSGQNGI
KKDSDLKNSKYLKSVSVTNGEIKVVGQGSLENYSYTLKPSKNGQTITWETQCSGDDTSLFPTNFCS

Nucleotide


Download         Length: 441 bp        

>NTDB_id=725797 NYR89_RS02700 WP_279446232.1 549471..549911(+) (pilA) [Actinobacillus arthritidis strain CCUG24862]
ATGCAAAAAATCAGATTAATTAGACCGCTTGCTAAGGCTTTTACATTAATTGAATTAATGATCGTTATCGCGATTATTGC
CATTCTTGCCACCATTGCGATCCCTTCCTATAACAGTTATACCCAAAAAGCCGCACTTTCGGAATTACTTTCCGCTTCAT
CTTCTTATAAAACTGATGTTGAAATTTGTGTGTATAACACCGGAGATATTAATGCCTGTAACAGCGGACAAAATGGGATT
AAAAAGGATAGTGATCTTAAAAATTCAAAATATTTGAAATCGGTATCGGTAACAAATGGAGAAATAAAAGTGGTTGGGCA
AGGGAGCTTGGAGAATTACAGCTATACACTAAAACCGAGTAAAAATGGACAAACTATCACGTGGGAAACGCAATGTAGTG
GTGATGATACGAGTTTATTCCCGACAAACTTCTGTTCATAG

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(43-132)

Prokaryotic N-terminal methylation motif (N_methyl)

(12-33)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

68.919

100

0.699
  pilA Haemophilus influenzae 86-028NP

57.241

99.315

0.568
  pilA Haemophilus influenzae Rd KW20

55.862

99.315

0.555
  pilA Vibrio campbellii strain DS40M4

34.868

100

0.363