Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NYR87_RS04850 Genome accession   NZ_CP103831
Coordinates   1076171..1077556 (-) Length   461 a.a.
NCBI ID   WP_279481829.1    Uniprot ID   -
Organism   Actinobacillus equuli subsp. haemolyticus strain 1812     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1071171..1082556
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYR87_RS04820 (NYR87_04815) lptE 1071784..1072278 (+) 495 WP_279481823.1 LPS assembly lipoprotein LptE -
  NYR87_RS04825 (NYR87_04820) holA 1072379..1073404 (+) 1026 WP_279481824.1 DNA polymerase III subunit delta -
  NYR87_RS04830 (NYR87_04825) yacG 1073481..1073669 (-) 189 WP_005623139.1 DNA gyrase inhibitor YacG -
  NYR87_RS04835 (NYR87_04830) coaE 1073651..1074289 (-) 639 WP_279443720.1 dephospho-CoA kinase -
  NYR87_RS04840 (NYR87_04835) - 1074311..1074985 (-) 675 WP_279481826.1 prepilin peptidase -
  NYR87_RS04845 (NYR87_04840) pilC 1074985..1076178 (-) 1194 WP_279481827.1 type II secretion system F family protein Machinery gene
  NYR87_RS04850 (NYR87_04845) pilB 1076171..1077556 (-) 1386 WP_279481829.1 GspE/PulE family protein Machinery gene
  NYR87_RS04855 (NYR87_04850) pilA 1077577..1078014 (-) 438 WP_279481831.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  NYR87_RS04860 (NYR87_04855) radA 1078195..1079574 (+) 1380 WP_279481833.1 DNA repair protein RadA Machinery gene
  NYR87_RS04865 (NYR87_04860) pepB 1079640..1080923 (+) 1284 WP_279481950.1 aminopeptidase PepB -
  NYR87_RS04870 (NYR87_04865) - 1081051..1081986 (+) 936 WP_279481835.1 KpsF/GutQ family sugar-phosphate isomerase -
  NYR87_RS04875 (NYR87_04870) greB 1081996..1082484 (+) 489 WP_279481837.1 transcription elongation factor GreB -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52497.15 Da        Isoelectric Point: 5.6685

>NTDB_id=725773 NYR87_RS04850 WP_279481829.1 1076171..1077556(-) (pilB) [Actinobacillus equuli subsp. haemolyticus strain 1812]
MQYSVCDMNSQQLFEISESLWQKNCDEKHILLRYLAVPIQENDNTLWLAIDDMNNLSACEIFAFIAHKTIEPVLISSDEL
KYLLNQLSPEQQSLYEETELNYQSPDNLELLNQNDPIIQILDNLFKFCLKQNASDIHFEPQKEKLLIRLRIDGVLHIYKS
LSFSLANRIITRLKLLAKLDISETRLPQDGQFNFKTVTAETLDFRVSSLPTSFGEKIVLRLQKNKPTHFDFLELGFSPEQ
KQLLLTALHQPQGLILVTGPTGSGKSITLYSALNYLNNSERHILTAEDPIEIEIEGIIQTQVNRAIQLDFSQLLRTFLRQ
DPDIIMLGEIRDRESAEIALRAAQTGHLVLSTLHTNDAPSAIERLLQLGVKEYEIKNSLQLIIAQRLLRKRCPQCGGKGC
DNCYHGYKGRTGIYQLLAKQGKFFEKSTAYLDYDSLRASAEQKLAANITTVDEVKRVLGDG

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=725773 NYR87_RS04850 WP_279481829.1 1076171..1077556(-) (pilB) [Actinobacillus equuli subsp. haemolyticus strain 1812]
ATGCAGTATTCGGTTTGTGATATGAACAGCCAGCAGCTATTTGAAATTTCTGAATCGTTATGGCAAAAAAATTGTGATGA
AAAACATATTTTATTACGTTATTTGGCTGTGCCGATTCAAGAAAATGACAATACGCTATGGCTTGCAATTGATGATATGA
ATAATTTGAGTGCTTGTGAAATTTTTGCATTTATTGCGCATAAAACAATTGAACCTGTTTTGATTTCCTCCGATGAATTG
AAGTACTTACTTAATCAACTTTCGCCGGAACAACAATCTTTATATGAAGAAACCGAGTTAAATTATCAATCGCCGGATAA
TTTAGAGCTACTCAATCAAAATGATCCTATTATTCAGATTTTAGATAATTTATTTAAATTTTGTTTAAAACAGAATGCTT
CAGATATTCACTTTGAACCGCAAAAGGAAAAGTTGCTGATTAGATTACGTATTGATGGGGTATTGCACATCTATAAAAGT
TTATCGTTTAGTTTAGCTAATCGTATTATCACGAGGCTTAAATTGTTAGCTAAATTGGATATTAGTGAGACACGCTTACC
ACAAGACGGGCAGTTTAATTTTAAGACGGTAACAGCTGAGACATTAGATTTTCGAGTGTCCAGTTTACCGACTAGCTTCG
GTGAGAAAATAGTCTTGCGCTTGCAAAAGAATAAGCCTACCCATTTTGATTTTTTGGAATTAGGTTTTTCGCCGGAACAA
AAGCAATTATTACTTACAGCGTTACACCAGCCACAAGGGTTGATTCTGGTTACGGGACCAACAGGCAGTGGCAAAAGTAT
TACTTTATATAGTGCGTTAAATTATTTAAATAATAGCGAACGGCATATTCTGACTGCAGAAGACCCGATTGAAATTGAGA
TAGAAGGGATTATCCAAACTCAAGTAAATCGTGCAATTCAATTAGACTTTAGCCAATTACTCAGAACATTTTTACGTCAG
GATCCGGACATTATTATGTTAGGTGAAATCCGTGATCGGGAAAGTGCCGAAATTGCATTAAGAGCAGCGCAAACAGGACA
TCTTGTACTTTCTACTTTACATACGAATGATGCGCCGTCTGCGATTGAACGCTTATTACAATTGGGAGTTAAAGAGTACG
AAATTAAGAATTCTCTGCAACTGATCATTGCACAGCGTTTATTACGTAAACGTTGTCCGCAATGTGGCGGAAAAGGGTGT
GATAACTGTTATCACGGTTATAAAGGGCGAACAGGAATTTACCAATTGCTTGCCAAGCAAGGTAAATTTTTTGAAAAATC
AACCGCTTATTTGGATTATGACAGCTTACGAGCCAGTGCCGAACAAAAGTTAGCGGCTAACATAACAACAGTAGACGAAG
TAAAGCGGGTGTTAGGTGATGGTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(129-390)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(27-93)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

72.707

99.349

0.722
  pilB Haemophilus influenzae Rd KW20

56.984

97.831

0.557
  pilB Haemophilus influenzae 86-028NP

56.541

97.831

0.553
  pilB Vibrio parahaemolyticus RIMD 2210633

40.04

100

0.432
  pilB Vibrio campbellii strain DS40M4

40.412

100

0.425
  pilB Legionella pneumophila strain ERS1305867

39.634

100

0.423
  pilB Vibrio cholerae strain A1552

40.041

100

0.419
  pilB Acinetobacter baumannii D1279779

38.889

100

0.41
  pilB Acinetobacter baylyi ADP1

37.827

100

0.408