Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NYR87_RS04855 Genome accession   NZ_CP103831
Coordinates   1077577..1078014 (-) Length   145 a.a.
NCBI ID   WP_279481831.1    Uniprot ID   -
Organism   Actinobacillus equuli subsp. haemolyticus strain 1812     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1072577..1083014
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYR87_RS04830 (NYR87_04825) yacG 1073481..1073669 (-) 189 WP_005623139.1 DNA gyrase inhibitor YacG -
  NYR87_RS04835 (NYR87_04830) coaE 1073651..1074289 (-) 639 WP_279443720.1 dephospho-CoA kinase -
  NYR87_RS04840 (NYR87_04835) - 1074311..1074985 (-) 675 WP_279481826.1 prepilin peptidase -
  NYR87_RS04845 (NYR87_04840) pilC 1074985..1076178 (-) 1194 WP_279481827.1 type II secretion system F family protein Machinery gene
  NYR87_RS04850 (NYR87_04845) pilB 1076171..1077556 (-) 1386 WP_279481829.1 GspE/PulE family protein Machinery gene
  NYR87_RS04855 (NYR87_04850) pilA 1077577..1078014 (-) 438 WP_279481831.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  NYR87_RS04860 (NYR87_04855) radA 1078195..1079574 (+) 1380 WP_279481833.1 DNA repair protein RadA Machinery gene
  NYR87_RS04865 (NYR87_04860) pepB 1079640..1080923 (+) 1284 WP_279481950.1 aminopeptidase PepB -
  NYR87_RS04870 (NYR87_04865) - 1081051..1081986 (+) 936 WP_279481835.1 KpsF/GutQ family sugar-phosphate isomerase -
  NYR87_RS04875 (NYR87_04870) greB 1081996..1082484 (+) 489 WP_279481837.1 transcription elongation factor GreB -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 15427.77 Da        Isoelectric Point: 8.0828

>NTDB_id=725774 NYR87_RS04855 WP_279481831.1 1077577..1078014(-) (pilA) [Actinobacillus equuli subsp. haemolyticus strain 1812]
MKLMIKPVQRAFTLIELMIVIAIIAILATVAIPSYNNYTKKAALSELLAASSAYKTDVEICIYNTGETKSCSSGTNGIKN
GDDLKNSKYLASVSVASGIITVDGKGSVAGYGYTLTPDYSNKTLTWKTTCSGEDKSLFPAAFCSE

Nucleotide


Download         Length: 438 bp        

>NTDB_id=725774 NYR87_RS04855 WP_279481831.1 1077577..1078014(-) (pilA) [Actinobacillus equuli subsp. haemolyticus strain 1812]
ATGAAACTCATGATCAAGCCTGTACAACGGGCATTTACACTGATTGAATTAATGATTGTAATTGCAATCATCGCAATTTT
GGCAACAGTAGCCATTCCTTCTTATAACAATTACACCAAAAAGGCAGCATTATCTGAGCTTTTGGCGGCATCTTCGGCGT
ATAAAACGGATGTGGAAATTTGTATTTATAACACCGGCGAGACAAAAAGTTGTTCTAGTGGAACGAATGGCATTAAAAAT
GGCGATGATCTTAAAAATTCTAAGTATTTAGCTTCTGTCTCTGTAGCTTCCGGAATCATAACTGTGGATGGTAAGGGAAG
TGTAGCAGGATACGGCTATACACTTACTCCGGATTATTCAAATAAAACGTTAACTTGGAAAACGACTTGTTCAGGAGAAG
ATAAAAGTTTATTCCCAGCCGCGTTTTGTTCGGAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(8-31)

Pilin (bacterial filament) (Pilin)

(41-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

64.138

100

0.641
  pilA Haemophilus influenzae Rd KW20

58.904

100

0.593
  pilA Haemophilus influenzae 86-028NP

56.849

100

0.572
  comP Acinetobacter baylyi ADP1

40.141

97.931

0.393