Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NYR86_RS04830 Genome accession   NZ_CP103830
Coordinates   1098490..1099875 (-) Length   461 a.a.
NCBI ID   WP_279623645.1    Uniprot ID   -
Organism   Actinobacillus equuli subsp. equuli strain 2499     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1093490..1104875
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYR86_RS04800 (NYR86_04800) lptE 1094096..1094590 (+) 495 WP_278225775.1 LPS assembly lipoprotein LptE -
  NYR86_RS04805 (NYR86_04805) holA 1094698..1095723 (+) 1026 WP_279471321.1 DNA polymerase III subunit delta -
  NYR86_RS04810 (NYR86_04810) yacG 1095800..1095988 (-) 189 WP_005623139.1 DNA gyrase inhibitor YacG -
  NYR86_RS04815 (NYR86_04815) coaE 1095970..1096608 (-) 639 WP_279439093.1 dephospho-CoA kinase -
  NYR86_RS04820 (NYR86_04820) - 1096630..1097304 (-) 675 WP_279623643.1 prepilin peptidase -
  NYR86_RS04825 (NYR86_04825) pilC 1097304..1098497 (-) 1194 WP_279623644.1 type II secretion system F family protein Machinery gene
  NYR86_RS04830 (NYR86_04830) pilB 1098490..1099875 (-) 1386 WP_279623645.1 GspE/PulE family protein Machinery gene
  NYR86_RS04835 (NYR86_04835) pilA 1099896..1100333 (-) 438 WP_279623646.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  NYR86_RS04840 (NYR86_04840) radA 1100514..1101893 (+) 1380 WP_279443725.1 DNA repair protein RadA Machinery gene
  NYR86_RS04845 (NYR86_04845) pepB 1101959..1103245 (+) 1287 WP_279623676.1 aminopeptidase PepB -
  NYR86_RS04850 (NYR86_04850) - 1103373..1104308 (+) 936 WP_278225782.1 KpsF/GutQ family sugar-phosphate isomerase -
  NYR86_RS04855 (NYR86_04855) greB 1104318..1104806 (+) 489 WP_005611555.1 transcription elongation factor GreB -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52499.15 Da        Isoelectric Point: 5.3508

>NTDB_id=725746 NYR86_RS04830 WP_279623645.1 1098490..1099875(-) (pilB) [Actinobacillus equuli subsp. equuli strain 2499]
MQYSVCDMNSQQLFEISDSLWQKNCDEKHVLLRYLAMPIQENDDMLWLAIDDMNNLSACEIFAFIAHKTIEPVLISPDEL
KYLLNQLSPEQQSVYEETELNYQSPDNLELLNQNDPIIQILDNLFKFCLKQNASDIHFEPQKEKLQIRLRIDGVLHIYKS
LSFSLANRIITRLKLLAKLDISETRLPQDGQFNFKTVTAETLDFRVSSLPTSFGEKIVLRLQKNKPTHFDFLELGFSPEQ
KQLLLAALHQPQGLILVTGPTGSGKSITLYSALNYLNNSERHILTAEDPIEIEIEGIIQTQVNRAIQLDFSQLLRTFLRQ
DPDIIMLGEIRDQESAEIALRAAQTGHLVLSTLHTNDAPSAIERLLQLGIKEYEIKNSLQLIIAQRLLRKRCPQCGGKGC
DNCYHGYKGRTGIYQLLAKQGKFFEKSTAYLDYDSLRASAEQKLAANITTVDEVKRVLGDG

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=725746 NYR86_RS04830 WP_279623645.1 1098490..1099875(-) (pilB) [Actinobacillus equuli subsp. equuli strain 2499]
ATGCAGTATTCGGTTTGTGATATGAACAGCCAGCAGCTATTTGAAATTTCAGACTCGTTGTGGCAAAAAAATTGTGATGA
AAAACATGTTTTATTACGTTATTTGGCTATGCCGATTCAAGAAAATGACGATATGCTATGGCTTGCAATTGATGATATGA
ATAATTTGAGTGCTTGTGAAATTTTTGCATTTATTGCGCATAAAACAATTGAACCTGTTTTGATTTCCCCCGATGAATTG
AAGTATTTACTTAATCAACTTTCGCCGGAACAACAATCCGTATATGAAGAAACCGAGTTAAATTATCAATCGCCGGATAA
TTTAGAACTACTCAATCAAAATGACCCTATTATTCAGATTTTAGATAATTTATTTAAATTTTGCTTAAAGCAAAATGCTT
CGGACATTCACTTTGAACCGCAAAAAGAGAAGCTACAGATTAGATTACGTATTGATGGGGTATTGCACATCTATAAAAGT
TTATCGTTTAGTTTAGCTAATCGTATTATCACGAGGCTTAAATTGTTAGCTAAATTAGATATTAGCGAAACACGTTTACC
ACAAGACGGCCAATTTAATTTTAAGACGGTAACGGCTGAGACATTAGATTTTCGAGTGTCCAGTTTACCGACTAGCTTCG
GTGAGAAAATAGTCTTGCGCTTGCAAAAGAATAAGCCTACCCATTTTGATTTTTTGGAATTAGGTTTTTCGCCGGAACAA
AAACAATTACTACTGGCAGCACTACATCAGCCACAAGGGCTGATTCTGGTGACAGGACCTACGGGTAGTGGCAAAAGTAT
TACTTTATATAGTGCGTTAAATTATTTAAATAATAGCGAACGGCATATTCTGACGGCAGAAGACCCGATTGAAATTGAGA
TAGAAGGGATTATCCAAACTCAAGTTAATCGTGCTATTCAATTGGATTTTAGTCAATTACTCAGAACATTTTTACGTCAG
GATCCGGACATTATTATGTTAGGTGAAATCCGTGATCAGGAAAGTGCTGAAATCGCATTAAGAGCAGCGCAAACAGGACA
TCTTGTACTTTCTACTTTACATACGAATGATGCGCCGTCTGCGATTGAGCGTTTACTACAATTAGGGATTAAAGAGTATG
AGATTAAGAATTCGCTGCAACTGATTATCGCACAGCGTTTATTACGTAAACGTTGTCCGCAATGTGGCGGAAAAGGGTGT
GATAACTGTTATCACGGTTATAAAGGGCGAACAGGAATTTACCAATTGCTTGCCAAGCAAGGTAAATTTTTTGAAAAATC
AACCGCTTATTTGGATTATGACAGCTTACGAGCCAGTGCCGAACAAAAGTTAGCGGCTAACATAACAACAGTAGACGAAG
TAAAGCGGGTGTTAGGTGATGGTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(129-390)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(29-92)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

72.489

99.349

0.72
  pilB Haemophilus influenzae Rd KW20

57.206

97.831

0.56
  pilB Haemophilus influenzae 86-028NP

56.763

97.831

0.555
  pilB Legionella pneumophila strain ERS1305867

39.431

100

0.421
  pilB Vibrio campbellii strain DS40M4

40

100

0.421
  pilB Vibrio parahaemolyticus RIMD 2210633

40.125

100

0.419
  pilB Vibrio cholerae strain A1552

39.627

100

0.414
  pilB Acinetobacter baylyi ADP1

37.827

100

0.408
  pilB Acinetobacter baumannii D1279779

38.683

100

0.408