Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NYR86_RS04835 Genome accession   NZ_CP103830
Coordinates   1099896..1100333 (-) Length   145 a.a.
NCBI ID   WP_279623646.1    Uniprot ID   -
Organism   Actinobacillus equuli subsp. equuli strain 2499     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1094896..1105333
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYR86_RS04810 (NYR86_04810) yacG 1095800..1095988 (-) 189 WP_005623139.1 DNA gyrase inhibitor YacG -
  NYR86_RS04815 (NYR86_04815) coaE 1095970..1096608 (-) 639 WP_279439093.1 dephospho-CoA kinase -
  NYR86_RS04820 (NYR86_04820) - 1096630..1097304 (-) 675 WP_279623643.1 prepilin peptidase -
  NYR86_RS04825 (NYR86_04825) pilC 1097304..1098497 (-) 1194 WP_279623644.1 type II secretion system F family protein Machinery gene
  NYR86_RS04830 (NYR86_04830) pilB 1098490..1099875 (-) 1386 WP_279623645.1 GspE/PulE family protein Machinery gene
  NYR86_RS04835 (NYR86_04835) pilA 1099896..1100333 (-) 438 WP_279623646.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  NYR86_RS04840 (NYR86_04840) radA 1100514..1101893 (+) 1380 WP_279443725.1 DNA repair protein RadA Machinery gene
  NYR86_RS04845 (NYR86_04845) pepB 1101959..1103245 (+) 1287 WP_279623676.1 aminopeptidase PepB -
  NYR86_RS04850 (NYR86_04850) - 1103373..1104308 (+) 936 WP_278225782.1 KpsF/GutQ family sugar-phosphate isomerase -
  NYR86_RS04855 (NYR86_04855) greB 1104318..1104806 (+) 489 WP_005611555.1 transcription elongation factor GreB -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 15543.87 Da        Isoelectric Point: 7.5775

>NTDB_id=725747 NYR86_RS04835 WP_279623646.1 1099896..1100333(-) (pilA) [Actinobacillus equuli subsp. equuli strain 2499]
MKFVFKPIQQAFTLIELMIVIAIIAILATVAIPSYNNYTKKAALSELLAASSAYKTDVEICIYNTGEVKNCSSGTNGIKN
GNDIKNSKYLDSISVASGVITVKGKGSVDGYGYTLTPDYSNKTLTWETACLGEDTSLFPAAFCSK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=725747 NYR86_RS04835 WP_279623646.1 1099896..1100333(-) (pilA) [Actinobacillus equuli subsp. equuli strain 2499]
ATGAAATTCGTATTCAAGCCTATACAGCAGGCATTTACCCTGATTGAATTAATGATTGTAATTGCAATCATTGCTATTTT
GGCAACGGTAGCCATTCCTTCTTATAACAATTACACCAAAAAGGCGGCATTATCCGAACTGTTAGCGGCATCTTCCGCTT
ATAAAACAGATGTGGAAATTTGTATTTATAACACGGGAGAGGTAAAAAACTGTTCTAGCGGAACGAATGGCATCAAAAAT
GGTAACGATATCAAAAACTCTAAGTATTTAGATTCTATTTCTGTAGCTTCCGGTGTAATAACGGTGAAGGGGAAAGGGAG
TGTAGATGGGTATGGCTATACGCTTACACCGGATTATTCAAATAAAACGCTAACTTGGGAGACGGCTTGTTTAGGCGAGG
ATACTAGTCTATTCCCTGCCGCGTTTTGTTCGAAATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(41-128)

Prokaryotic N-terminal methylation motif (N_methyl)

(9-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

65.517

100

0.655
  pilA Haemophilus influenzae Rd KW20

55.479

100

0.559
  pilA Haemophilus influenzae 86-028NP

56.429

96.552

0.545
  comP Acinetobacter baylyi ADP1

40.152

91.034

0.366