Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NYR82_RS04995 Genome accession   NZ_CP103827
Coordinates   1085882..1087267 (-) Length   461 a.a.
NCBI ID   WP_279442710.1    Uniprot ID   -
Organism   Actinobacillus equuli subsp. haemolyticus strain 3090     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1080882..1092267
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYR82_RS04960 (NYR82_04965) lptE 1081488..1081982 (+) 495 WP_279442705.1 LPS assembly lipoprotein LptE -
  NYR82_RS04965 (NYR82_04970) - 1081987..1082133 (-) 147 WP_278225056.1 hypothetical protein -
  NYR82_RS04970 (NYR82_04975) holA 1082090..1083115 (+) 1026 WP_279442706.1 DNA polymerase III subunit delta -
  NYR82_RS04975 (NYR82_04980) yacG 1083192..1083380 (-) 189 WP_115589824.1 DNA gyrase inhibitor YacG -
  NYR82_RS04980 (NYR82_04985) coaE 1083362..1084000 (-) 639 WP_279442707.1 dephospho-CoA kinase -
  NYR82_RS04985 (NYR82_04990) - 1084022..1084696 (-) 675 WP_279442708.1 prepilin peptidase -
  NYR82_RS04990 (NYR82_04995) pilC 1084696..1085889 (-) 1194 WP_279442709.1 type II secretion system F family protein Machinery gene
  NYR82_RS04995 (NYR82_05000) pilB 1085882..1087267 (-) 1386 WP_279442710.1 GspE/PulE family protein Machinery gene
  NYR82_RS05000 (NYR82_05005) pilA 1087288..1087725 (-) 438 WP_279442711.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  NYR82_RS05005 (NYR82_05010) radA 1087906..1089285 (+) 1380 WP_279442712.1 DNA repair protein RadA Machinery gene
  NYR82_RS05010 (NYR82_05015) pepB 1089351..1090638 (+) 1288 Protein_976 aminopeptidase PepB -
  NYR82_RS05015 (NYR82_05020) - 1090766..1091701 (+) 936 WP_279442713.1 KpsF/GutQ family sugar-phosphate isomerase -
  NYR82_RS05020 (NYR82_05025) greB 1091711..1092199 (+) 489 WP_005611555.1 transcription elongation factor GreB -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52513.16 Da        Isoelectric Point: 5.4993

>NTDB_id=725670 NYR82_RS04995 WP_279442710.1 1085882..1087267(-) (pilB) [Actinobacillus equuli subsp. haemolyticus strain 3090]
MQYSVCDMNSRQLFEISDVLWQKNCDEKHILLRYLAMPIQENDDTLWLAIDDMNNLSACEIFAFIAHKTIEPVLISSDEL
KYLLNQLSPEQQSVYEETELNYQSPDNLELLNQNDPIIQILDNLFKFCLKQNASDIHFEPQKEKLQIRLRIDGVLHIYKS
LSFSLANRIITRLKLLAKLDISETRLPQDGQFNFKTVTAETLDFRVSSLPTSFGEKIVLRLQKNKPTHFDFLELGFSPEQ
KQLLLAALHQPQGLILVTGPTGSGKSITLYSALNYLNNSERHILTAEDPIEIEIEGIIQTQVNRAIQLDFSQLLRTFLRQ
DPDIIMLGEIRDQESAEIALRAAQTGHLVLSTLHTNDAPSAIERLLQLGIKEYEIKNSLQLIIAQRLLRKRCPQCGGKGC
DNCYHGYKGRTGIYQLLAKQGKFFEKSTAYLDYDSLRASAEQKLAANITTVDEVKRVLGDG

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=725670 NYR82_RS04995 WP_279442710.1 1085882..1087267(-) (pilB) [Actinobacillus equuli subsp. haemolyticus strain 3090]
ATGCAGTATTCGGTTTGTGATATGAACAGCCGGCAGCTATTTGAAATTTCAGATGTATTGTGGCAGAAAAATTGTGATGA
AAAACATATTTTATTACGTTATTTGGCTATGCCGATTCAAGAAAATGACGATACGCTATGGCTTGCAATTGATGATATGA
ATAATTTGAGTGCTTGTGAAATTTTTGCATTTATTGCGCATAAAACAATTGAACCTGTTTTGATTTCCTCCGATGAATTG
AAGTATTTACTTAATCAACTTTCGCCGGAACAACAATCTGTATATGAAGAAACCGAGTTAAATTATCAATCGCCGGATAA
TTTAGAACTACTCAATCAAAATGACCCTATTATTCAGATTTTAGATAATTTATTTAAATTTTGCTTAAAGCAAAATGCTT
CGGACATTCACTTTGAACCGCAAAAAGAGAAGCTACAGATTAGATTACGTATTGATGGGGTATTACATATCTATAAAAGT
TTATCGTTTAGCTTAGCTAATCGTATTATCACGAGGCTTAAATTGTTAGCTAAATTAGATATTAGCGAAACACGTTTACC
ACAAGACGGCCAGTTTAATTTTAAGACGGTAACAGCTGAGACATTAGATTTTCGAGTGTCCAGTTTACCGACTAGCTTCG
GTGAGAAAATAGTCTTGCGCTTGCAAAAGAATAAGCCTACCCATTTTGATTTTTTGGAATTAGGTTTTTCGCCGGAACAA
AAACAATTACTACTGGCAGCACTACATCAGCCACAAGGGCTGATTCTGGTGACAGGACCTACGGGTAGTGGCAAAAGTAT
TACTTTATATAGTGCGTTAAATTATTTAAATAATAGCGAACGGCATATTCTGACGGCAGAAGACCCGATTGAAATTGAGA
TAGAAGGGATTATCCAAACTCAAGTTAATCGTGCTATTCAATTGGATTTTAGTCAATTACTCAGAACTTTTTTACGTCAG
GATCCGGACATTATTATGTTAGGTGAAATCCGTGATCAGGAAAGTGCTGAAATCGCATTAAGAGCAGCGCAAACAGGACA
TCTTGTACTTTCTACTTTACATACGAATGATGCGCCGTCTGCGATTGAGCGTTTACTACAATTAGGGATTAAAGAGTATG
AGATTAAGAATTCGCTGCAACTGATTATCGCACAGCGTTTATTACGTAAACGTTGTCCGCAATGTGGCGGAAAAGGGTGT
GATAACTGTTATCACGGTTATAAAGGGCGAACAGGAATTTACCAATTGCTTGCCAAGCAAGGTAAATTTTTTGAAAAATC
AACCGCTTATTTGGATTATGACAGCTTACGAGCCAGTGCCGAACAAAAGTTAGCGGCTAACATAACAACAGTAGACGAAG
TAAAGCGGGTGTTAGGTGATGGTTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(29-92)

Type II/IV secretion system protein (T2SSE)

(129-390)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

72.489

99.349

0.72
  pilB Haemophilus influenzae Rd KW20

57.428

97.831

0.562
  pilB Haemophilus influenzae 86-028NP

56.984

97.831

0.557
  pilB Vibrio parahaemolyticus RIMD 2210633

39.638

100

0.427
  pilB Vibrio campbellii strain DS40M4

40.206

100

0.423
  pilB Legionella pneumophila strain ERS1305867

39.431

100

0.421
  pilB Vibrio cholerae strain A1552

39.627

100

0.414
  pilB Acinetobacter baumannii D1279779

38.683

100

0.408
  pilB Acinetobacter baylyi ADP1

37.903

100

0.408