Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NYR82_RS05000 Genome accession   NZ_CP103827
Coordinates   1087288..1087725 (-) Length   145 a.a.
NCBI ID   WP_279442711.1    Uniprot ID   -
Organism   Actinobacillus equuli subsp. haemolyticus strain 3090     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1082288..1092725
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYR82_RS04975 (NYR82_04980) yacG 1083192..1083380 (-) 189 WP_115589824.1 DNA gyrase inhibitor YacG -
  NYR82_RS04980 (NYR82_04985) coaE 1083362..1084000 (-) 639 WP_279442707.1 dephospho-CoA kinase -
  NYR82_RS04985 (NYR82_04990) - 1084022..1084696 (-) 675 WP_279442708.1 prepilin peptidase -
  NYR82_RS04990 (NYR82_04995) pilC 1084696..1085889 (-) 1194 WP_279442709.1 type II secretion system F family protein Machinery gene
  NYR82_RS04995 (NYR82_05000) pilB 1085882..1087267 (-) 1386 WP_279442710.1 GspE/PulE family protein Machinery gene
  NYR82_RS05000 (NYR82_05005) pilA 1087288..1087725 (-) 438 WP_279442711.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  NYR82_RS05005 (NYR82_05010) radA 1087906..1089285 (+) 1380 WP_279442712.1 DNA repair protein RadA Machinery gene
  NYR82_RS05010 (NYR82_05015) pepB 1089351..1090638 (+) 1288 Protein_976 aminopeptidase PepB -
  NYR82_RS05015 (NYR82_05020) - 1090766..1091701 (+) 936 WP_279442713.1 KpsF/GutQ family sugar-phosphate isomerase -
  NYR82_RS05020 (NYR82_05025) greB 1091711..1092199 (+) 489 WP_005611555.1 transcription elongation factor GreB -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 15528.86 Da        Isoelectric Point: 7.5779

>NTDB_id=725671 NYR82_RS05000 WP_279442711.1 1087288..1087725(-) (pilA) [Actinobacillus equuli subsp. haemolyticus strain 3090]
MKFVFKPIQQAFTLIELMIVIAIIAILATVAIPSYNNYTKKAALSELLAASSAYKTDVEICIYNTGEVKNCSSGTNGIKN
GDDIKNSKYLASISVASGVITVKGKGSVDGYGYTLTPDYSNKTLTWETACLGEDTSLFPAAFCSR

Nucleotide


Download         Length: 438 bp        

>NTDB_id=725671 NYR82_RS05000 WP_279442711.1 1087288..1087725(-) (pilA) [Actinobacillus equuli subsp. haemolyticus strain 3090]
ATGAAATTCGTATTCAAGCCTATACAGCAGGCATTTACTCTGATTGAATTAATGATTGTAATTGCAATCATTGCTATTTT
GGCAACGGTAGCCATTCCTTCTTATAACAATTACACCAAAAAGGCGGCATTATCCGAACTGTTAGCGGCATCTTCCGCTT
ATAAAACAGATGTGGAAATTTGTATTTATAACACGGGAGAGGTAAAAAACTGTTCTAGCGGAACGAATGGTATCAAAAAT
GGTGACGATATTAAAAACTCTAAGTATTTAGCCTCTATTTCTGTAGCTTCCGGTGTAATAACTGTGAAGGGGAAAGGGAG
TGTAGATGGGTATGGATATACGCTTACACCGGATTATTCAAATAAAACGCTAACTTGGGAGACAGCTTGTTTAGGCGAGG
ATACTAGTTTATTCCCTGCCGCGTTCTGTTCGAGATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(41-128)

Prokaryotic N-terminal methylation motif (N_methyl)

(9-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

65.517

100

0.655
  pilA Haemophilus influenzae Rd KW20

56.164

100

0.566
  pilA Haemophilus influenzae 86-028NP

56.429

96.552

0.545
  pilA Vibrio campbellii strain DS40M4

37.589

97.241

0.366